Search Results - Koch’s Postulates

Identification and management of Bipolaris sorokiniana in wheat and barley in Southeast Kazakhstan by Kharipzhanova, Aidana, Dutbayev, Yerlan, Erginbas Orakci, Gul, Dababat, Abdelfattah A.S., Korkulu, Ş.G., Aydın, S., Paulitz, Timothy, Özer, Göksel, Bozoglu, Tuğba, Zholdoshbekova, Sezim, Sultanova, Nadira, Kokhmetova, Alma

“…Molecular analysis of B. sorokiniana isolates via species-specific primers (COSA_F/COSA_R) from infected plant tissues confirmed their identification. Koch postulates were fulfilled for B. sorokiniana isolates Kz 48, 60, and 82 on Steklovidnaya 24 winter wheat and Symbat spring barley varieties. …”
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First report of Curvularia lunata associated with stem disease of cassava by Msikita, W., Yaninek, John S., Ahounou, M., Baimey, H., Fagbemissi, R.

“…Boedijn (confirmed also by the International My-cological Institute, Surrey, U.K.). To complete Koch's postulates, stem pieces of four cassava cultivars (Agric, Tchukunochi, TMS 30572, and Ben 86052), were disinfected in hot water (52°C for 5 min), transplanted in sterilized sand, and maintained in a greenhouse under natural light at 28 to 30°C. …”
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Ceratobasidium sp. AG-A, root pathogen of Calibrachoa hybrida by Borrelli, Nicolás Pablo, Moreno, María Virginia, Stenglein, Sebastian, Stancanelli, Santiago, Wright, Eduardo R., Rivera, Marta Carolina

“…One of the isolates was selected and identified using morphological and molecular markers, while its pathogenicity was confirmed by Koch’s postulates. The obtained results allow concluding that Ceratobasidium sp. …”
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Evidence that Xylella fastidiosa is the Causal Agent of Almond Leaf Scorch Disease in Alicante, Mainland Spain (Iberian Peninsula) by Marco-Noales, Ester, Barbé, Silvia, Monterde, Adela, Navarro-Herrero, Inmaculada, Ferrer, Amparo, Dalmau, Vicente, Aure, Cristina M., Domingo-Calap, María L., Landa, Blanca B., Roselló, Montserrat

“…With the fulfillment of Koch’s postulates, we have demonstrated that X. fastidiosa is the causal agent of the almond leaf scorch disease in the Alicante outbreak…”
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First report of Colletotrichum cliviicola causing anthracnose disease of cowpea (Vigna unguiculata L. Walp) in Nigeria by Dada, A.O., Dania, V.O., Olaniyi, O., Abberton, Michael T., Ortega Beltran, A.

“…A bi-locus phylogenetic tree revealed that the isolates belong to the species C. cliviicola (Fig. 3) when compared with existing sequences in the GenBank (Table 1). To fulfill Koch’s postulates, pathogenicity of each of the four C. cliviicola isolates was confirmed on 2-week-old cowpea plants cv. …”
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First report of Phakopsora pachyrhizi on soybean causing rust in Tanzania by Murithi, H., Beed, Fenton D., Madata, C.S., Haudenshield, J.S., Hartman, G.L.

“…Free surviving urediniospores were dislodged from 12 samples and inoculated onto susceptible soybean cultivar Williams 82, which produced sporulating SBR lesions after 2 weeks of incubation in a detached-leaf assay. Thus, Koch's postulates were completed. This is the first report of P. pachyrhizi causing rust on soybean in Tanzania. …”
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First report of leaf blight of Taro (Colocasia esculenta) caused by Phytophthora colocasiae in Ghana by Omane, E., Oduro, K.A., Cornelius, E., Opoku, I., Akrofi, Andrews, Sharma, K., Kumar, P. Lava, Bandyopadhyay, Ranajit

“…On the basis of morphological characteristics and nucleotide homology, the isolates were identified as P. colocasiae. To fulfill Koch's postulates, 30 leaf discs from 3-month-old plants were inoculated with 10 μl of a suspension of 3 × 105 zoospores per ml (2). …”
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Identification of Xanthomonas vasicola (formerly X. campestris pv. musacearum), causative organism of banana xanthomonas wilt, in Tanzania, Kenya and Burundi by Carter, B.A., Reeder, R., Mgenzi, S.R, Mwangi, M., Aritua, Valentine, Smith, J., Beed, Fenton D., Kinyua, Z.M., Nakato, V., Miller, S., Mbaka, J.N., Doyle, K., Lewis Ivey, M.L.

“…All cultureswere identified to species level at FERA by fatty acid profiling (MIDI system)and DNA analysis using X. vasicola specific primers (Aritua et al.,unpublished data) and partial sequencing of the gyrase B gene (Parkinsonet al., 2007). Koch’s postulates were fulfilled for all strains at FERA bystem inoculation of banana plants (height approximately 30 cm) with abacterial suspension (200 lL with ~107 cfu ⁄ mL) under controlled environmentalconditions (minimum temperature 27ºC). …”
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Confirmation of Bacterial Leaf Streak Caused by Xanthomonas oryzae pv. oryzicola on Rice in Madagascar by Poulin, L., Raveloson, H., Sester, M., Raboin, L.M., Silué, D., Koebnik, R., Szurek, B.

“…Symptomatic leaf tissues were ground and plated on non-selective PSA medium, resulting in colonies with typical Xanthomonas morphology that were confirmed as X. oryzae pv. oryzicola by multiplex PCR typing (3), thus fulfilling Koch's postulates. Finally, the five strains were subjected to gyrB sequencing upon PCR amplification using the universal primers XgyrB1F (5′-ACGAGTACAACCCGGACAA-3′) and XgyrB1R (5′-CCCATCARGGTGCTGAAGAT-3′). …”
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First report of mango malformation disease caused by Fusarium tupiense in Senegal by Senghor, A.L., Sharma, K., Kumar, P. Lava, Bandyopadhyay, Ranajit

“…Phylogenetic analysis inferred from the Clustalw alignment of TEF-1α sequences clustered I4 and I17 isolates with F. tupiense (3). To confirm Koch's postulates, 2-year-old healthy mango seedlings var. …”
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Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina by Bernanrdi Lima, Nelson, Conforto, Erica Cinthia, Serri, Dannae Lilia, Campilongo Mancilla, Ernesto Javier, Stenglein, Sebastian, Vargas Gil, Silvina

“…Pathogenicity testing showed these isolates were pathogenic to alfalfa and Koch's postulates were verified by re-isolating the pathogen.…”
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Phomopsis head rot caused by Diaporthe helianthi : A new disease on sunflower in Argentina by Colombo, Denis Nahuel, Figueruelo, Andrea Mariana, Paniego, Norma Beatriz, Comerio, Ricardo Mario, Corro Molas, Andres Ezequiel

“…Pathogenicity experiments confirmed the presence of similar disease symptoms in inoculated sunflower heads, and D. helianthi was consistently reisolated from these organs. Our Koch's postulates testing results on heads constitute the first confirmed report that D. helianthi is the cause of Phomopsis head rot on sunflower in Argentina.…”
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First report of Xanthomonas oryzae pv. oryzicola causing bacterial leaf streak of rice in Burundi by Afolabi, O., Milan, B., Amoussa, R., Koebnik, R., Poulin, L., Szurek, B., Habarugira, G., Bigirimana, J., Silué, D.

“…Re-isolation of bacteria from symptomatic leaves yielded colonies with the typical Xanthomonas morphology that were confirmed by multiplex PCR to be X. oryzae pv. oryzicola, thus fulfilling Koch's postulates. Bur_1 has been deposited in the Collection Française de Bactéries Phytopathogènes as strain CFBP 8170 ( http://www.angers-nantes.inra.fr/cfbp/ ). …”
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First report of crown rot caused by fusarium algeriense on wheat in Kyrgyzstan by Özer, Göksel, Erper, İsmail, Imren, Mustafa, Bozoglu, Tuğba, Ozdemir, Fatih, Dababat, Abdelfattah A.

“…The pathogen was successfully reisolated from the symptomatic tissues, confirming Koch’s postulates. To the best of our knowledge, this is the first report of crown rot caused by F. algeriense on wheat in Kyrgyzstan. …”
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First report of Dactylonectria alcacerensis, Dactylonectria macrodidyma and Ilyonectria liriodendri associated with black-foot disease of grapevine in Argentina by Longone, Maria Valeria, Escoriaza, Maria Georgina, Paolinelli, Marcos, Gramaje, David

“…All fungi were re-isolated only from necrotic lesions of root and base of inoculated canes, fulfilling Koch's postulates. No symptoms were observed on the control plants. …”
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Etiología de la enfermedad punta de cigarro en plantaciones de banana en las provincias de Salta y Jujuy by Ortiz, Claudio Manuel

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Caracterización de la bacteria Xylella fastidiosa en plantaciones de olivo de Argentina by Tolocka, Patricia

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Performance of varieties of Gboma (Solanum macrocarpon) under disease pressure with organic amendment in Southern Benin by Sikirou, R., Hoteigni, J., Godonou, I., James, B., Grehounou, G., Assogba-Komlan, F.

“…This is in Benin the first record of C. capsici as pathogen of S. macrocarpon with positive Koch postulate reaction and of the Abidjan Gboma as resistant variety of S. macrocarpon to C. capsici. …”
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First report of Xanthomonas prunicola causing bacterial leaf streaks on wheat in Argentina by Martino, Julia Andrea, Fernandez, Franco Daniel, Pozzi, Elizabeth Alicia, Alberione, Enrique Javier, Bainotti, Carlos Tomas, Marquez, Nathalie, Tolocka, Patricia, Salines, Nicolas, Gomez, Dionisio Tomas, Donaire, Guillermo Manuel, Conci, Luis Rogelio, Alemandri, Vanina Maria

“…A bacterial suspension containing 108 CFU mL−1 grown for 48 h on LB medium at 28 °C was injected into three-week-old leaves of wheat plants to fulfill Koch’s postulates. After 5 days, plants showed symptoms of chlorosis, streaks and then necrosis on the leaves. …”
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Epidemiología, caracterización y control de Stemphylium vesicarium, agente causal de mancha marrón en perales by Tudela, Marisa Andrea Aluminé

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