Resultados de búsqueda - Knokke~
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Diagnostic kit for rice blight resistance
Publicado 2019“…Specifically, we include aSWEETpromoter database, RT–PCR primers for detectingSWEETinduction, engineered reporter rice lines to visualize SWEET protein accumulation and knock-out rice lines to identify virulence mechanisms in bacterial isolates. …”
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Journal Article -
Control de Conyza spp. con “doble golpe” de herbicidas en pulverizaciones terrestres contrastantes: boquillas hidroneumáticas versus hidráulicas
Publicado 2021“…Según Papa y Tuesca (2012) “el doble golpe aplicado al manejo de malezas comenzó a utilizarse en la década de 1960, cuando la siembra directa aún estaba en desarrollo y en Argentina se difundió ampliamente en los últimos años como consecuencia de la importancia que adquirió la rama negra (Conyza spp.) así como de la difusión que la empresa Syngenta realizó de la práctica registraba bajo la denominación de Doble Knock Down (DKD).” La técnica se ha utilizado especialmente para malezas “duras”, y estado avanzado de desarrollo, como la especie Conyza spp., conocida vulgarmente como “rama negra”.…”
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Informe técnico -
Effects of deletion of the ac109 gene of Autographa californica nucleopolyhedrovirus on interactions with mammalian cells
Publicado 2017“…We demonstrated that although a Ac109 knock out virus maintained its ability to enter into BHK-21 cells, there was a marked reduction in the expression efficiency of the nuclear transgene. …”
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Artículo -
Bridging the Policy and Investment Gap for Payment for Ecosystem Services: Learning from Costa Rican Experience and Roads Ahead
Publicado 2020“…As forests are lost so are all of the knock-on economic, environmental, and social benefits of ecosystem services provided by those forests (e.g. sequestrating carbon dioxide, regulating hydrological systems, maintaining soil quality, preventing erosion, and hosting biodiversity). …”
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Reporte técnico -
A molecular analysis of the interaction between the biocontrol fungus Clonostachys rosea and the cereal pathogen F. graminearum
Publicado 2021“…Growth rate analysis of prz1 knock-out mutants showed decreased mycelial growth on agar plates supplemented with CaCl2 or SDS. …”
H2 -
Rice Chalky Grain 5 regulates natural variation for grain quality under heat stress
Publicado 2022“…These findings are supported by increased chalkiness of OsCG5 knock-out (KO) mutants relative to wildtype (WT) under heat stress. …”
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Journal Article -
The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far?
Publicado 2020“…Therefore, the ALS gene has become a model sequence target to improve our technological skills for more precise CRISPR/Cas nucleotide base substitution in plants, which is essential for modulation/modification of gene function as opposed to the more general gene knock out obtained by indels in conventional genome editing studies. …”
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Artículo -
Self-(In)compatibility Systems: Target Traits for Crop-Production, Plant Breeding, and Biotechnology
Publicado 2020“…Molecular level studies have enabled SI to SC transitions (and vice versa) to be intentionally manipulated using marker assisted breeding and targeted approaches based on transgene integration, silencing, and more recently CRISPR knock-out of SI-related factors. These scientific advances have, in turn, provided a solid basis to implement new crop production and plant breeding practices. …”
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Artículo -
Effect of herbicides and Sesbaniaco-culture on weed management and grain yield of direct seeded rice variety Khumal-4 at Khumaltar condition, Nepal
Publicado 2016“…Sesbania seed were sown at 30 kg/ha as co-culture with rice on the same day as rice was sown by broadcasting. The Sesbania was knocked down at 30 DAS using 2, 4-D.The yield of rice in 2013 was less than that of 2012 which could be due to the less rainfall and uneven rain distribution during the rice season. …”
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Journal Article -
Development of a naked eye CRISPR-Cas12a and -Cas13a multiplex point-of-care detection of genetically modified swine
Publicado 2023“…As a proof-of-concept, reliable multiplex RAVI-CRISPR detection of genome-edited pigs was demonstrated, exhibiting 100% sensitivity and specificity for the analysis of CD163 knockout, lactoferrin (LF) knock-in, and wild-type pig samples. This portable naked-eye multiplex RAVI-CRISPR detection platform can provide accurate point-of-care screening of genetically modified animals and infectious diseases in resource-limited settings.…”
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Journal Article -
Monitoring the agri-food system in Myanmar: Agricultural crop traders – April 2023 survey
Publicado 2023“…Policies introduced to track exports more closely and bolster foreign currency reserves may add frictions to international crop trade that have knock-on effects through crop value chains.…”
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Brief -
CRISPR-Cas9 and CRISPR-Cpf1 mediated targeting of a stomatal developmental gene EPFL9 in rice
Publicado 2017“…The present study utilized CRISPR/Cas9 and CRISPR-Cpf1 technology to knock out an early developmental gene EPFL9(Epidermal Patterning Factor like-9, a positive regulator of stomatal development inArabidopsis) orthologue in rice. …”
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Journal Article -
Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea
Publicado 2019“…Inoculation of tomato leaves with B. cinerea increases SlPLC2 transcript levels. We knocked‐down the expression of SlPLC2 by virus‐induced gene silencing and plant defense responses were analyzed upon B. cinerea inoculation. …”
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Artículo -
From climate risk to resilience: Unpacking the economic impacts of climate change in Mozambique
Publicado 2023“…Damages to Mozambique’s transport sector are likely to have knock-on effects to other sectors and can have significant regional implications, as the country serves as a conduit for landlocked neighboring countries. …”
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Informe técnico -
From climate risk to resilience: Unpacking the economic impacts of climate change in Zambia
Publicado 2023“…In road infrastructure, the projected higher occurrence of flooding, especially in Lusaka Province, could have a knock-on effect for the rest of the economy, particularly if it damages key international corridors passing through this region and/or affects domestic supply chains. …”
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Informe técnico -
Identification of novel high molecular weight glutenin subunit mutants in bread wheat (Triticum aestivum L.)
Publicado 2018“…This type of mutation generates null alleles, therefore they are easily detectable by a low cost protocol like SDS-PAGE. The potential use of knock-out (null alleles) and SDS-PAGE size altered mutants for HMW-GS in wheat quality and nutrition is discussed.…”
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Artículo -
Efecto del distanciamiento entre surcos sobre las enfermedades de la colza
Publicado 2024Enlace del recurso
Informe técnico -
The role of mast cells and mast cell mediators in the development of atopic dermatitis in a mouse model
Publicado 2014“…To further investigate the role of MCs and its mediators in the progression of AD, a low-calcemic vitamin D3 analog (MC903) was used to induce AD-like symptoms locally on the ears of two different knock-out (KO) mouse strains. The first strain was Wsh-/- mice deficient in MCs. …”
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Second cycle, A2E -
Monitoring Aedes populations for arboviruses, Wolbachia, insecticide resistance and its mechanisms in various agroecosystems in Benin
Publicado 2024“…Insecticide resistance was assessed phenotypically, by permethrin (0.75%) exposure of Adults (F0) using World Health Organization (WHO) bioassay protocols, and at the molecular level, using TaqMan (RT)-qPCR assays for assessing knock-down resistance (kdr) mutations (F1534C, V1016G/I, and S989P) and the expression levels of eight detoxification genes (P450s from the CYP9 and CYP6 families, carboxylesterases and glutathione-S-transferases). …”
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Journal Article