Resultados de búsqueda - ELISAVA~

TpUB05, a Homologue of the Immunodominant Plasmodium falciparum Protein UB05, Is a Marker of Protective Immune Responses in Cattle Experimentally Vaccinated against East Coast Feve... por Dinga, Jerome Nyhalah, Wamalwa, Mark, Njimoh, Dieudonné Lemuh, Njahira, Moses N., Djikeng, Appolinaire, Skilton, Robert A., Titanji, Vincent Pryde Kehdingha, Pellé, Roger

“…Polyclonal antiserum against recombinant TpUB05 were raised in rabbits and used for further analysis by Western blotting, ELISA, immunolocalization and in vitro infection neutralization assay. …”
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Diagnostic Tools Applied by ICARDA’s Germplasm Health Unit (GHU) for Detection of Pests and Diseases Transmitted via Legume and Cereal Seeds por Kumari, Safaa, Moukahel, Abdulrahman, El Miziani, Inaam, Darwish, Iman

“…(i) visual inspection and floating test for stored insects, (ii) centrifuge washing, agar plate and freezing blotter tests for fungi, (iii) TBIA and ELISA for viruses, (iv) nematode extraction test for nematodes, and (v) agglutination test for bacteria. …”
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Resistance to African swine fever virus among African domestic pigs appears to be associated with a distinct polymorphic signature in the RelA gene and upregulation of RelA transcr... por Bisimwa, P.N., Ongus, J.R., Tonui, R., Bisimwa, E.B., Steinaa, Lucilla

“…Additionally, the inflammatory cytokine levels of IFN-α, IL-10, and TNF-α at both the protein and the mRNA transcript levels were measured using ELISA and Real-Time PCR, respectively. The resulting data was used in correlation analysis to assess the association between cytokine levels and the RelA gene expression. …”
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Estimating seroprevalence and variation to four tick-borne infections and determination of associated risk factors in cattle under traditional mixed farming system in Mbeere Distri... por Gachohi, John M., Ngumi, P.N., Kitala, P.M., Skilton, Robert A.

“…Prevalences of serum antibodies were determined using the enzyme-linked immunosorbent assay (ELISA) technique. The relationship between TBDs seroprevalence and the risk factors was assessed by multivariable analysis using standard logistic regression models and mixed models using the farm as a random effect. …”
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Exploring community-based reporting of livestock abortions for Rift Valley fever and brucellosis surveillance in Uganda: a pilot study por Walekhwa, A.W., Conlan, A.J.K., Atim, S.A., Ademun, A.R., Hasahya, Emmanuel, Wood, J.L.N., Mugisha, L.

“…Of these, 184 samples were tested via ELISA for RVF virus antibodies (IgG and IgM), while all 200 were screened for anti-Brucella (IgG) antibodies using validated commercial kits. …”
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Seroprevalences of vector-transmitted infections of small-holder dairy cattle in coastal Kenya por Maloo, S.H., Thorpe, W.R., Kiio, G., Ngumi, P., Rowlands, G.J., Perry, Brian D.

“…Sera were tested for antibodies to Theileria parva, using the schizonts-antigen indirect fluorescent-antibody (IFA) test and to antibodies for Babesia bigemina and antigens to Anaplasma marginale by enzyme-linked immunosorbent assay (ELISA). Packed-cell volume (PCV) also was measured. …”
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Sero-epidemiology of Peste des petits ruminants virus infection in Turkana County, Kenya por Kihu, S.M., Gachohi, John M., Ndungu, E.K., Gitao, G.C., Bebora, L.C., Njenga, J.M., Wairire, G.G., Maingi, N., Wahome, R.G., Ireri, R.

“…PPRV competitive enzyme-linked immuno-sorbent assay (c-ELISA) analysed the presence of antibodies in the samples. …”
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Enhancing resistance against African weevils through development of transgenic sweetpotato cultivars (Ipomoea batatas (L.) Lam.) expressing cry7Aa1, cry3Ca1 and ET33-34 genes. por Manrique Trujillo, S., Wamalwa, N.L., Díaz, D., Reano, R., Tovar, J.C., Prentice, K., Ormachea, M., Kreuze, Jan F., Machuka, J., Ghislain, M.

“…Transcriptional activity and Cry protein accumulation are being tested in leaves and storage roots by Real time PCR and DAS-ELISA respectively.…”
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A longitudinal survey of African swine fever in Uganda reveals high apparent disease incidence rates in domestic pigs, but absence of detectable persistent virus infections in bloo... por Muhangi, D., Masembe, C., Emanuelson, Ulf, Boqvist, Sofia, Mayega, L., Ademun, R.O., Bishop, Richard P., Ocaido, M., Berg, M., Ståhl, Karl

“…Three pigs tested positive for ASFV using real-time PCR, but none tested positive for ASFV specific antibodies using two different commercial ELISA tests. There was no evidence for existence of pigs that were long-term carriers for the virus based on the analysis of blood and serum as there were no seropositive pigs and the only three ASFV DNA positive pigs were acutely infected and were linked to outbreaks reported by farmers during the study. …”
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Aflatoxin exposure among young children in urban low-income areas of Nairobi and association with child growth por Kiarie, G.M., Domínguez Salas, Paula, Kang'ethe, S.K., Grace, Delia, Lindahl, Johanna F.

“…Samples of foods were taken from the household or from the retailer for analysis using competitive enzyme-linked immunosorbent assay (ELISA). Laboratory results for aflatoxin levels in the food samples collected were used to calculate the daily aflatoxin intake, according to the results from the dietary assessment. …”
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Prevalence of aflatoxin in feeds and cow milk from five counties in Kenya por Senerwa, D.M., Sirma, A.J., Mtimet, Nadhem, Kang'ethe, Erastus K., Grace, Delia, Lindahl, Johanna F.

“…Dairy feed concentrates and cattle milk were collected twice (dry season and rainy season) from 285 dairy farmers in the five counties and analysed for AFB1 and AFM1, using competitive enzyme-linked immunosorbent assay (ELISA). In the five counties, the proportion of farmers who fed cattle with dairy concentrates varied from zero to 68%. …”
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Aflatoxins in dairy cattle feed in Senegal por Marshall, Karen, Missohou, Ayao, Tebug, Stanly Fon, Kagera, I., Grace, Delia, Lindahl, Johanna F.

“…Aflatoxin levels were determined by a commercial competitive ELISA. Feeds with the highest levels of aflatoxin were purchased concentrate (with an average of 50 ppb, and a range of 0 to 305 ppb), ground-nut cake (45ppb, 0 to 187ppb) and millet bran (37ppb, 0 to186 ppb). …”
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The sero-epidemiology of Rift Valley fever in people in the Lake Victoria Basin of western Kenya por Cook, Elizabeth A.J., Grossi-Soyster, E.N., Glanville, William A. de, Thomas, Lian F., Kariuki, S., Bronsvoort, B.M. de C., Wamae, C.N., LaBeaud, A.D., Fèvre, Eric M.

“…Structured questionnaire tools were used to collect information on demographic data, health, and risk factors for zoonotic disease exposure. Indirect ELISA on serum samples determined seropositivity to RVFV. …”
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Estimation of tsetse challenge and its relationship with trypanosomosis incidence in cattle kept under pastoral production systems in Kenya por Bett, Bernard K., Irungu, P., Nyamwaro, Sospeter S., Murilla, G., Kitala, P., Gathuma, J., Randolph, Thomas F., McDermott, John J.

“…Blood meals were also collected from some of the flies and their sources identified using enzyme-linked immunosorbent assay (ELISA). Tsetse challenge was obtained as a product of tsetse density, trypanosome prevalence and the proportion of blood meals obtained from cattle. …”
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First report of the occurrence of East African cassava mosaic virusUganda (EACMVUG) in Angola por Kumar, P. Lava, Akinbade, S.A., Dixon, Alfred G.O., Mahungu, N.M., Mutunda, M.P., Kiala, D., Legg, James P.

“…Sixty-three of 65 samples obtained from these fields testedpositive in TAS-ELISA using monoclonal antibody SCR 20. PCR wasperformed using primer pairs previously described for the specificidentification of ACMV, EACMV, EACMV-UG, East African cassavamosaic Cameroon virus (EACMCV) and Indian cassava mosaic virus(ICMV) (Ogbe et al., 2003), East African cassava mosaic Malawi virus(EACMMV) (Zhou et al., 1998), East African cassava mosaic Zanzibarvirus (EACMZV) (Were et al., 2004) and South African cassava mosaicvirus (SACMV) in mixed infections. …”
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Relationship between natural occurrence of banana streak badnavirus and symptom expression, relative concentration of viral antigen, and yield characteristics of some micropropagat... por Dahal, G., Ortíz, R., Tenkouano, A., Hughes, J., Thottappilly, G., Vuylsteke, D.R., Lockhart, B.E.L.

“…The characteristics evaluated were the natural incidence of BSV based on symptoms and virus indexing, relative concentration of BSV antigens in leaf tissues determined by ELISA, and some growth and yield descriptors. Virus occurrence and symptom expression, as well as the relative concentration of BSV antigens, fluctuated greatly between seasons during the cropping cycle, being high during the rainy season and low or negligible during the hot dry season. …”
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Évaluation du potentiel prolifératif de six cultivars de bananier (cv. AAB, ABB, et AAA) par macropropagation en République Démocratique du Congo. por Bangata, B.M., Mobambo, M.K., Kasongo, M., Shungu, D., Vuvu, K., Vangu, P., Omondi, Bonaventure Aman Oduor, Staver, Charles

“…Méthodologie et résultats : Le test ELISA a été utilisé en vue de cribler les échantillons des plantes, en rapport avec la maladie de Bunchy Top (BBTD). …”
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Temporal and spatial dynamics of Tomato spotted wilt virus and its vector in a potato crop in Argentina por Salvalaggio, Andrea Eugenia, Lopez Lambertini, Paola Maria, Cendoya, Maria Gabriela, Huarte, Marcelo Atilio

“…The presence of plants with symptoms of TSWV was evaluated by visual observation and DAS-ELISA analysis to confirm the virus infection. Thrips species were collected from leaves and inflorescences and identified under stereomicroscope. …”
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Germplasm evaluation of the native cultivar “Torrontes Riojano” (Vitis vinífera L.) collected in the Northwest of Argentina por Torres, Maria Del Rocio, Gualpa, Jose Luis, Lanza Volpe, Melisa, Blanco, Maria Evangelina, Gomez Talquenca, Gonzalo

“…Virus diseases were screened by ELISA tests, and six microsatellites were used to confirm varietal identity: VVMD5, VVMD7, VVMD27, ssrVrZAG 62, ssrVrZAG 79 and VVS2. …”
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Spiraea sp. new natural host of Plum pox virus (Sharka) por Piglionico, Damián Gustavo, Ojeda, Eva Maria, Lucero, Vanesa Angelica, Farrando, Roberto Jes, Porcel, Laura Beatriz, Picca, Cecilia Nelida, Marini, Diana Beatriz

“…Symptomatic leaves were analyzed for PPV by DAS-ELISA with positive results. The presence of the virus was confirmed by conventional and qRT-PCR and the use of “GF-305” biological indicator plants inoculated by T-bud grafting with buds from the Spiraea. …”
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