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A survey of aflatoxin B1 in maize and awareness of aflatoxins in Vietnam by Hu Suk Lee, Hung Nguyen-Viet, Lindahl, Johanna F., Ha Minh Thanh, Tran Ngoc Khanh, Le Thi Thu Hien, Grace, Delia

“…A total of 2,370 samples were collected from six provinces and analysed using ELISA. Among collected samples, 799 samples (33.71%, 95% confidence interval (CI): 31.81-35.66%) and 687 samples (28.98%, 95%CI: 27.17-30.86%) had levels above 2 µg/kg and 5 µg/kg, respectively [range from below limit of detection (LOD) to 34.8 µg/kg; of the samples above LOD, the mean was 13.1 µg/kg and median was 11.2 µg/kg]. …”
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Xanthomonas campestris pv. musacearum: a major constraint to banana, plantain and enset production in central and east Africa over the past decade by Nakato, V., Mahuku, George S., Coutinho, Teresa A.

“…Xcm can also be identified using direct antigen coating enzyme-linked immunosorbent assay (DAC-ELISA), species-specific polymerase chain reaction (PCR) using GspDm primers and lateral flow devices that detect latent infections. …”
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Distinct strains of the re‐emergent Cassava common mosaic virus (Genus: Potexvirus) infecting cassava in Argentina by Zanini, A.A, Cuéllar, Wilmer Jose, Celli, M.G., Luque, V.A., Medina, R., Conci, V.C., Di Feo, L.V. del

“…In this work we successfully purified the virus and developed a double-antibody sandwich (DAS) ELISA test from an Argentinean field-isolate of CsCMV, to extend the diagnostics of the disease. …”
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Metagenomic analysis of viruses associated with maize lethal necrosis in Kenya by Wamaitha, M. Jane, Nigam, Deepti, Maina, Solomon, Stomeo, Francesca, Wangai, Anne, Njuguna, Joyce N., Holton, Timothy A., Wanjala, Bramwel W., Wamalwa, Mark, Lucas, Tanui, Djikeng, Appolinaire, García Ruiz, H.

“…Both groups of plants often tested negative for SCMV by ELISA. We used next-generation sequencing to identify viruses associated to maize lethal necrosis in Kenya through a metagenomics analysis. …”
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Occurrence of aflatoxin in agricultural produce from local markets in Burundi and Eastern Democratic Republic of Congo by Udomkun, Patchimaporn, Mutegi, C., Assfaw Wossen, Tesfamicheal, Atehnkeng, J., Nabahungu, N.L., Njukwe, E., Vanlauwe, Bernard, Bandyopadhyay, Ranajit

“…In order to investigate the levels of aflatoxin, crop samples were analyzed using a single step lateral flow immunochromatographic assay (Reveal Q+), while enzyme‐linked immune‐sorbent assay (ELISA) was used to analyze aflatoxin‐M1 in milk, yogurt, and cheese samples. …”
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Association between Rift Valley fever virus seroprevalences in livestock and humans and their respective intra-cluster correlation coefficients, Tana River County, Kenya by Bett, Bernard K., Lindahl, Johanna F., Sang, R., Wainaina, M., Kairu-Wanyoike, S., Bukachi, S., Njeru, I., Karanja, J., Ontiri, E., Njenga, M.K., Wright, D., Warimwe, G.M., Grace, Delia

“…Serum samples were collected and screened for anti-RVFv immunoglobulin G (IgG) antibodies using inhibition IgG enzyme-linked immunosorbent assay (ELISA). Data collected were analysed using generalised linear mixed effects models, with herd/household and village being fitted as random variables. …”
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Pre-harvest management is a critical practice for minimizing aflatoxin contamination of maize by Mahuku, George S., Nzioki, H.S., Mutegi, C., Kanampiu, F.K., Narrod, C., Makumbi, Dan

“…To understand sources of aflatoxin contamination for home grown maize, we collected 789 maize samples from smallholder farmers’ fields in Eastern and South Western, two regions in Kenya representing high and low aflatoxin risk areas, respectively, and determined aflatoxin B1 (AFB1) using ELISA with specific polyclonal antibodies. AFB1 was detected in 274 of the 416 samples from Eastern Kenya at levels between 0.01 and 9091.8 μg kg−1 (mean 67.8 μg kg−1). …”
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Detection of circulating antigens for Taenia spp. in pigs slaughtered for consumption in Nairobi and surroundings, Kenya by Akoko, James M., Macleod, Ewan, Thomas, Lian F., Alarcón, Pablo, Kang'ethe, Erastus K., Kivali, Velma, Muloi, Dishon M., Muinde, Patrick, Murungi, Maurice K., Gachoya, Julius M., Fèvre, Eric M.

“…The samples were tested using an antigen ELISA to determine the prevalence of infection with Taenia spp. …”
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Accuracy of traditional and novel serology tests for predicting cross-protection in foot-and-mouth disease vaccinated cattle by Brito, Barbara Patricia, Perez, Andrés Maximiliano, Capozzo, Alejandra Victoria

“…Foot-and-mouth disease virus (FMDV) antigenic-match between vaccine and field viruses has traditionally been estimated in vitro by computing the r1 value using virus neutralization test (VNT) or ELISA titers. In this study we compared the accuracy in predicting cross-protection between the r1 value estimated by VNT and two recently developed tests that measure IgG subtypes and avidity. …”
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Susceptibility of Prunus rootstocks to natural infection of Plum pox virus and effect of mineral oil treatments by Vidal, Eduardo, Moreno, A., Bertolini, Edson, Pérez-Panadés, J., Carbonell, Emilio A., Cambra, Mariano

“…Samples from both plots were analysed by double-antibody sandwich indirect enzyme-linked immunosorbent assay (DASI-ELISA) and spot real-time RT-PCR. Under high inoculum pressure, the assayed rootstocks exhibited significant differences in their susceptibility to natural infection. …”
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Evaluation of the Susceptibility of Different Prunus Rootstocks to Natural Infection of Plum Pox Virus-T by Caglayan, Kadriye, Serce, Cigdem Uluba, Gazel, Mona, Kaya, Kamuran, Cengiz, F. C., Vidal, Eduardo, Cambra, Mariano

“…Aphids collected from different rootstocks were tested individually by squash real-time RT-PCR and all rootstocks were regularly tested by DASI-ELISA. The largest aphid populations were observed at the end of May and the most abundant aphid species as averages over the two years were Myzus persicae (20.15%), Hyalopterus pruni (18.64%), Aphis craccivora (9.04%) and Aphis gossypii (8.36%). …”
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Potential vectors of Plum pox virus in the Eastern Mediterranean Region of Turkey by Kaya, Kamuran, Gazel, Mona, Serce, Cigdem Uluba, Elci, Eminur, Cengiz, Feza Can, Cambra, Mariano, Caglayan, Kadriye

“…Regularly collected plant samples and aphids were individually tested by DASI-ELISA and squash real-time RT-PCR, respectively. …”
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An Erwinia amylovora strain without plasmid pEA29 isolated in a nursery from hawthorn by Llop, Pablo, Donat, Victoria, López, María M., Cabrefiga, Jordi, Ruz, Lidia, Montesinos, Emilio, Palomo, José L., Rodriguez, Margarita

“…The strain was positive by ELISA using specific monoclonal antibodies, but not by PCR using primers designed from sequences of the plasmid pEA29. …”
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A New Graft Transmissible Disease Found in Nagami Kumquat by Navarro, Luis, Pina, José A., Ballester-Olmos, José F., Moreno, Pedro, Cambra, Mariano

“…Diseased kumquats gave negative reactions by ELISA using four different tristeza antisera.…”
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Turrialba; Vol. 33, no. 2

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Presence of recombinant Potato virus Y genotypes in Sweden by Youssef, Asmaa

“…To study the present genetic diversity of PVY in Swedish potato, 50 symptomatic potato samples from different parts of Sweden were selected for characterization of the infecting PVY genotypes using serology, multiplex-PCR, sequence analyses and inoculation tests. In DAS-ELISA, one sample was positive only for serotype O, 31 samples for only serotype N and 15 samples for both serotypes. …”

Characterisation of virus isolates associated with leaf yellowing in Swedish sugar-beet plants by Uddin, Mohammad Nasir

“…To test the presence of Beet mosaic virus (BtMV) several detection methods were applied, such as: mechanical inoculation/transmission test with sugar beet (Beta vulgaris), red beet (Beta vulgaris) cv Rubia, spinach (Spinacia oleracea) cv Long Standing Bloomsdale and lettuce (Lactuca sativa) cv Sonette; DAS-Enzyme-linked Immunosorbent Assay (DAS-ELISA) with polyclonal antibody; RT-PCR amplification with Potyviridae-specific universal primers Sprimer/M4 (Chen et al., 2001), and BtMV-specific primer pairs BM1/BM2 and BM1/BM3 (Glasa et al., 2003). …”
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Outbreaks of African swine fever in domestic pigs in Gulu district, Uganda by Tejler, Emma

“…Out of the six blood samples from pigs that survived outbreaks, three were ELISA-positive. The main transmission routes were trade of live pigs and pig products, especially at onset of an outbreak (introduction into a village). …”

Caracterización morfol+ogica de dieciocho accesiones del banco de germoplasma del genero Xanthosoma en condiciones del CENIAB-INTA, Managua, 2008 by Centeno Guevara, Bosco Rafael, Orozco Cisneros, Carlos Manuel

“…Las accesiones silvestres fueron precoces o tardías, las cultivadas de ciclo intermedio. 100% de las accesiones estaban infectadas con DsMV según prueba ELISA (180 dds). Los síntomas no fueron visibles en las accesiones silvestres. …”
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Determinar la situación de la Seroprevalencia de Rinotraquitis infecciosa bovina, en bovinos mayores de 8 meses de edad, en el Centro Experimental “Las Mercedes” Managua, durante e... by Flores Trejos, Julio Cesar, Siles Melgara, Massielidieth

“…El diagnostico utilizado para este trabajo fue la prueba de ELISA el único ente autorizado es el IPSA-Nicaragua basándose en el estudio de la enfermedad a través de la detección de anticuerpos en el suero sanguíneo. …”
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