Resultados de búsqueda - ELISAVA~

High-throughput characterization and phenotyping of resistance and tolerance to virus infection in sweetpotato por Kreuze, Jan F., Ramírez, D., Fuentes, S., Loayza, H., Ninanya, J., Rinza, J., David, M., Gamboa, S., Boeck, B. de, Díaz, F., Pérez, A., Silva, L., Campos, Hugo

“…Virus loads were assessed using ELISA, (RT-)qPCR, and loop-mediated isothermal amplification (LAMP) methods, and also through multispectral reflectance and canopy temperature collected using an unmanned aerial vehicle. …”
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Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Bean yellow mosaic virus (BYMV) in legumes applied by ICARDA’s GHU por Kumari, Safaa, Moukahel, Abdulrahman

“…This hybrid diagnostic technique is more sensitive than ELISA and RT-PCR alone. In addition, Immunocapture of virus particles step provides a simple method to isolate virus particles from plant tissue, particularly when inhibitory substances are present, and thus enables subsequent use of RT-PCR amplification. …”
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Seroprevalence of brucellosis, knowledge, and risky practices in dairy cattle owners and workers in Maekel and Debub regions, Eritrea por Efrem, G.H., Mihreteab, B., Ghebremariam, M.K., Tesfai, B., Mor, Siobhan M., Mamo, G.

“…Blood samples were tested using Rose Ben gal Plate Test, and positive samples were confirmed using competitive ELISA. Variation in knowledge scores by sociode mographic factors and practices were explored statistically. …”
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A retrospective investigation of abortion storm in Abergele goats, Waghimira Zone, Amhara Region, Ethiopia por Bahiru, A., Assefa, Ayalew, Gemeda, Biruk A., Desta, Hiwot, Tibebu, A., Sahle, A., Wieland, Barbara

“…The Rose Bengal Plate Test for Brucellosis, <i>Toxoplasma gondii</i> Antibody Test Kit for <i>Toxoplasma gondii</i>, and ELISA for <i>Chlamydophila abortus</i> and <i>Coxiella burnetii</i> were used to detect antibodies. …”
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Epidemiology of peste des petits ruminants in selected districts of Borena zone, Ethiopia por Kumbe, A., Negussie, H., Getachew, Y., Alemu, Biruk, Alemayehu, Gezahegn, Girma, S., Sibhatu, D., Emiyu, K., Waktole, H., Leta, S.

“…A total of 384 serum samples were collected randomly from sheep and goats and examined for the presence of PPRV antibodies using competition enzyme-linked immune sorbent assay (c-ELISA). Additionally, a retrospective analysis of five years of outbreak data was performed to provide insight into the spatial and temporal distribution of the disease. …”
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Detection of Four Legume Viruses (AMV, CMV, BYMV, PSbMV) in One Multiplex RT-PCR Test Applied at ICARDA’s Seed Health and Virology Laboratory por Kumari, Safaa, Moukahel, Abdulrahman

“…Currently, virus detection methods rely primarily on serological tests such as ELISA and TBIA to detect viral infections. However, screening each sample for all viruses via individual virus-specific tests is both cost-prohibitive and labor-intensive. …”
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Bacillus thuringiensis isolates from the Philippines: habitat distribution, delta-endotoxin diversity, and toxicity to rice stem borers (Lepidoptera: Pyralidae) por Theunis, W., Aguda, R.M., Cruz, W.T., Decock, C., Peferoen, M., Lambert, B., Bottrell, D.G., Gould, F.L., Litsinger, J.A., Cohen, M.B.

“…Polyclonal antibodies to six δ-endotoxin groups (Cry1A, Cry1B, Cry1C, Cry1D, Cry1E, and Cry3A) were used in enzyme-linked immunosorbent assays (ELISA) to characterize the toxins produced by each isolate. …”
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African swine fever virus antibody rapid detection assays evaluation: Evaluation of rapid lateral flow assays for the detection of antibodies against African swine fever virus for... por Lacasta, Anna, Oba, Peter, Dione, Michel M.

“…They include enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody test and immunoblotting. …”
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Optimization of multiplex PCR assay for the simultaneous detection of alfalfa mosaic virus (AMV) and southern bean mosaic virus (SBMV) por Dawit, Woubit, Mulatu, Fikerte, Hirko, Mignote, Negawo, Alemayehu T.

“…Conventional diagnostic methods, including Enzyme-Linked Immunosorbent Assay (ELISA) and single-target Reverse Transcription Polymerase Chain Reaction (RT-PCR), are limited by their inability to detect multiple viruses simultaneously, which is particularly challenging in crops prone to mixed infections. …”
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Development of a rapid, user-friendly, diagnostic test for Taenia solium cysticercosis por Doble, L.F., Fèvre, Eric M., Bronsvoort, B.M. de C., Harrison, L.J.S., Parkhouse, R.M.E., Toye, Philip G.

“…Several antibody and antigen ELISA assays have been developed, but the time, equipment and skills required to perform them makes them unsuitable for many of the situations where diagnostics are required. …”
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Concentrations of isometamidium in the sera of cattle challenged with drug-resistant Trypanosoma congolense por Eisler, M.C., Gault, E.A., Moloo, S.K., Holmes, P.H., Peregrine, A.S.

“…The relationship between serum concentrations of the prophylactic trypanocidal drug isometamidium chloride and protection against tsetse challenge with two populations of Trypanosoma congolense was investigated in Boran (Bos indicus) cattle, using an isometamidium-ELISA. Isometamidium chloride (Samorin) was administered to cattle at a dose rate of 1.0 mg/kg body weight by deep intramuscular injection. …”
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Comparison of African swine fever virus prevalence and risk in two contrasting pig-farming systems in South-west and Central Kenya por Okoth, Edward A., Gallardo, C., Macharia, Joseph M., Omore, Amos O., Pelayo, V., Bulimo, D.W., Arias, Marisa, Kitala, P., Baboon, K., Lekolol, I., Mijele, D., Bishop, Richard P.

“…No animals were sero-positive in either study site using the OIE indirect-ELISA and none of the animals sampled exhibited clinical symptoms of ASF. …”
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A survey of bovine cysticercosis/human taeniosis in Northern Turkana District, Kenya por Asaava, L.L., Kitala, P.M., Gathura, P.B., Nanyingi, M.O., Muchemi, G., Schelling, E.

“…The seroprevalence of cysticercosis in cattle was estimated at 16.7% (95% CI 13–20.9%) using a secretory–excretory antigen detection ELISA. There was poor agreement between meat inspection and serology (k = 0.025; p = 0.2797). …”
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The known and the unknowns: A multipathogen survey to identify diseases in cattle herds in Tanzania por Alonso, Silvia, Toye, Philip G., Jores, Joerg, Wakhungu, Judi W., Msalya, G., Grace, Delia, Unger, Fred

“…Sick cattle (n=400) were actively searched among cattle herds and blood samples collected along with information on farming practices. ELISA diagnostic kits were used to detect antibodies against the following cattle pathogens: contagious bovine pleuropneumonia (CBPP), anaplasmosis, theileriosis (x2), babesiosis, brucellosis, Rift Valley fever, Q fever, neospora, infectious bovine rhinotracheitis, bovine viral diarrhea (BVR) and bovine respiratory syncytial virus (BRSV). …”
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Application of PCR in heartwater epidemiology por Peter, T.F., O'Callaghan, C.J., Perry, Brian D., Medley, G.F., Mahan, S.

“…The few antigen detection tests described, brain biopsy, ELISA, and xenodiagnosis with blood or ticks, cannot reliably detect infection past the clinical stage of disease, and are too cumbersome and lengthy for large scale use in field and experimental epidemiologic studies. …”
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Antibiotic residues and heavy metals in pork at wet markets in Vietnam por Tran Thi Tuyet Hanh, Sinh Dang Xuan, Hung Nguyen-Viet, Unger, Fred, Chu Van Tuat, Ngan Tran Thi, Phuc Pham Duc

“…Tetracycline, fluoroquinolones, sulfonamide, chloramphenicol groups were firstly screened by ELISA and positive samples were quantified by liquid chromatography–mass spectrometry/mass spectrometry (LCMS/MS). …”
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Serological survey of porcine cysticercosis and associated risk factors in pigs slaughtered at Ndumbuini abattoir in Nairobi, Kenya por Akoko, James M., Macleod, E., Kang'ethe, Erastus K., Muinde, P., Alarcón, Pablo, Muloi, Dishon M., Gachoya, J., Fèvre, Eric M.

“…Blood samples were obtained from 700 pigs selected by systematic random sampling. Antigen ELISA was used to detect T. solium secretory antigen, while structured questionnaires, focus group discussions and observational tools were used to assess the risk factors. …”
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The association between exposure to aflatoxin, mutation in TP53,infection with hepatitis B virus, and occurrence of liver disease in a selected population in Hyderabad, India por Anitha, S., Raghunadharao, D., Waliyar, Farid, Sudini, H., Parveen, M., Rao, R., Kumar, P. Lava

“…This study examined mutations in TP53 by PCR-RFLP analysis and bymeasurement of an aflatoxin-albumin adduct as a biomarker for human exposure of aflatoxin B1 byindirect-competitive ELISA, in samples collected from healthy controls as well as patients with hepatitisin Hyderabad, Andhra Pradesh, India. …”
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Epidemiology of village chicken diseases: A longitudinal study on the magnitude and determinants of morbidity and mortality—The case of Newcastle and infectious bursal disease por Jarso, D.

“…Serology using HAI and I-ELISA test were conducted to determine the seroprevalence of NCD and IBD, respectively. …”
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Risk factors for leptospirosis seropositivity in slaughterhouse workers in western Kenya por Cook, Elizabeth A.J., Glanville, William A. de, Thomas, Lian F., Kariuki, S., Bronsvoort, B.M. de C., Fèvre, Eric M.

“…Questionnaires were used to collect information from workers on demographic data, health and hygiene practices in the slaughterhouse. A commercial ELISA detected antibodies to Leptospira spp. in serum samples and multilevel logistic regression analysis identified factors associated with leptospirosis seropositivity. …”
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