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Effect of trypanosomosis on the resumption of postpartum ovarian function and oestrus activity in Zebu cows by Tibbo, Markos, Mukasa-Mugerwa, E., Abebe, G., Mulatu, W.

“…This was done through the determination of milk progesterone levels using the enzyme linked immunosorbent assay (ELISA), packed cell volume (PCV), and darkground/phase contrast buffy coat examination to estimate the parasitic burden. …”
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The Emergence of Theileria parva in Jonglei State, South Sudan: Confirmation using molecular and serological diagnostic tools by Marcellino, Wani L., Salih, Diaeldin A., Njahira, Moses N., Ndiwa, Nicholas N., Araba, A., El Hussein, A.M., Seitzer, U., Ahmed, J.S., Bishop, Richard P., Skilton, Robert A.

“…Overall, the T. parva infection rate in R. appendiculatus was 25% as shown by nested PCR. ELISA was used to assess antibodies to T. parva, and the overall seroprevalence was 22.8%. …”
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Herd-level risk factors associated with Leptospira Hardjo infection in dairy herds in the southern Tohoku, Japan by Miyama, T., Watanabe, E., Ogata, Y., Urushiyama, Y., Kawahara, N., Makita, K.

“…Hardjo using a commercial ELISA kit, which detects both L. interrogans serovar Hardjo and L. borgpetersenii serovar Hardjo. …”
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Status of aflatoxin contamination in cow milk produced in smallholder dairy farms in urban and peri-urban areas of Nairobi County: A case study of Kasarani sub county, Kenya by Kagera, Irene, Kahenya, Peter, Mutua, Florence K., Anyango, Gladys, Kyallo, Florence, Grace, Delia, Lindahl, Johanna F.

“…A total of 84 milk samples were collected from small-holder dairy farms and analyzed for AFM1 using Enzyme-Linked Immunosorbent Assay (ELISA). Results and Discussion: Ninety nine percent of the samples (83/84) analysed were contaminated with AFM1. …”
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Real-time assay for quantitative detection of non-persistently transmitted Plum pox virus RNA targets in single aphids by Olmos, Antonio, Bertolini, Edson, Gil, Maite, Cambra, Mariano

“…The number of viruliferous aphids detected by real-time RT-PCR and nested RT-PCR in a single closed tube was similar in parallel assays, nevertheless the sensitivity provided by real-time RT-PCR was 100 times higher than nested RT-PCR and 1000 times higher than DASI-ELISA and conventional RT-PCR. The quantities of PPV-RNA targets detected in a single aphid ranged from 40 to more than 2 x 10(3) units. …”
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Post-transcriptional gene silencing of the p23 silencing suppressor of Citrus tristeza virus confers resistance to the virus in transgenic Mexican lime by Fagoaga, Carmen, Lopez, C., Hermoso-De-Mendoza, Alfonso, Moreno, Pedro, Navarro, Luis, Flores, R., Pena, Leandro

“…When graft- or aphid-inoculated with CTV, some propagations of these silenced lines were immune: they neither expressed symptoms nor accumulated virions and viral RNA as estimated by DAS-ELISA and Northern blot hybridization, respectively. …”
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Prevalencia serológica de pseudorabia (enfermedad de aujeszky) en Colombia. by Torres Ruíz, Myriam L., González Garzón, Guillermo

“…La comprobación de anticuerpos específicos se hizo por la técnica ELISA. Los resultados permitieron establecer una prevalencia puntual de 4.0 por ciento, determinándose que el mayor porcentaje de reactores serológicos (14.3 por ciento) se encontraba en la costa norte del país…”
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Epidemiology and characterization of newcastle disease in smallholder poultry in Mozambique by Perttula, Linda

“…The study included two different parts; a serology study of the prevalence of Newcastle disease by enzyme linked immunosorbent assay (ELISA) and a small survey about management by a questionnaire. …”

Immunoassays for detection of serum thymidine kinase 1 in dog lymphomas and carcinomas by Jagarlamudi, Kiran Kumar

“…These results are the first step in the development of an ELISA, which may provide clinical information about prognosis, risk of recurrence and effectiveness of antitumor therapy in veterinary medicine.…”

Comparison of productive performance in dairy goats with and without serological evidence of Mycobacterium avium subsp. paratuberculosis infection by Fiorentino, María Andrea, Zedde, A., Morsella, Claudia Graciela, Mendez, Luis Alberto, Antonacci, Liliana, Gagliostro, Gerardo Antonio, Paolicchi, Fernando

“…They were grouped on the basis of ELISAi, ZN and Map culture results in: Negative group (n=8) and Positive group (n=10). …”
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Desarrollo, optimización e implementación de un método de diagnóstico molecular para la detección del virus del sindrome reproductivo y respiratorio porcino (PRRSV) en planteles po...

“…Por lo tanto, en la actualidad se cuenta con las herramientas diagnósticas (ELISA y RT-PCR) que permiten realizar un diagnóstico certero, herramientas que son complementarias en el caso de utilizar suero como muestra. …”
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Ficha Iniciativa FIA : Desarrollo y evaluación de un sistema para el monitoreo a gran escala de las enfermedades de la vaca lechera

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Coxiella seroprevalence and risk factors in large ruminants in Bihar and Assam, India by Shome, R., Deka, Ram Pratim, Milesh, L., Sahay, S., Grace, Delia, Lindahl, Johanna F.

“…In this cross-sectional survey in the two states of Assam and Bihar, 520 households were interviewed for risk factors, and serum samples from 744 dairy animals were analyzed using ELISA as well as PCR. Out of the farms, 17.4% had at least one seropositive animal, with significantly higher seroprevalence in Bihar (27.1%) than Assam (5.8%); and significantly more sero-positive farms in urban areas (23.1%) than rural (12.2%). …”
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Persistence and recovery of introduced Rhizobium ten years after inoculation on Leucaena leucocephala grown on an Alfisol in southwetern Nigeria by Sanginga, N., Danso, S.K.A., Mulongoy, K., Ojeifo, A.A.

“…Serological typing of the nodules using the Enzyme-Linked-Immunosorbent Assay (ELISA) and intrinsic resistance to the streptomycin test revealed that most of the nodules (96%) formed on L. leucocephala in 1992 were by Rhizobium strains IRc 1045 and IRc 1050, which were inoculated in 1982. …”
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Detection of Toxoplasma gondii infection in semen of rams used for natural mating in commercial sheep farms in Tunisia by Rouatbi, M., Amairia, S., Lahmer, M., Lassoued, N., Rekik, Mourad, Wieland, Barbara, Mwacharo, Joram M., Gharbi, M.

“…Here, to estimate the prevalence of natural infection by Toxoplasma gondii, we used PCR to amplify and detect T. gondii DNA in semen from 92 rams of three breeds from four regions in Tunisia and seroprevalence was determined using a commercial ELISA kit. From the PCR amplifications, 51.09 ± 10.21% of the rams were tested positive for T. gondii with an overall seroprevalence of 39.13 ± 9.97%. …”
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Zoonotic pathogen seroprevalence in cattle in a wildlife–livestock interface, Kenya by Nthiwa, D., Alonso, Silvia, Odongo, D., Kenya, E., Bett, Bernard K.

“…Sera samples were collected from 1170 cattle belonging to 390 herds in all the zones and tested for antibodies against Brucella abortus and Leptospira interrogans serovar hardjo using ELISA kits. Data on putative risk factors for seropositivity of these pathogens in cattle were collected using a questionnaire. …”
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Occurrence, genetic diversity, and recombination of maize lethal necrosis disease-causing viruses in Kenya by Mwatuni, F.M., Nyende, A.B., Njuguna, Joyce N., Xiong, Z., Machuka, Eunice M., Stomeo, Francesca

“…The causative viruses of MLN were determined by RT-PCR using virus-specific primers and DAS-ELISA. Next-generation sequencing (NGS) data was generated, viral sequences identified, genetic diversity of MLN viruses was determined, and recombination was evaluated. …”
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Assessment of fungal contamination in fish feed from the Lake Victoria Basin, Uganda by Namulawa, V.T., Mutiga, Samuel K., Musimbi, F., Akello, S., Ng'ang'a, Fredrick M., Kago, Leah, Kyallo, Martina M., Harvey, Jagger J.W., Ghimire, Sita R.

“…Aflatoxin B1 (AFB1) and total fumonisin (combinations of B1, B2 and B3; hereinafter named fumonisin) levels in feed samples were determined by enzyme-linked immunosorbent assay (ELISA). A wide range of fungi, including toxigenic Aspergillus flavus and Fusarium verticillioides, were isolated from the fish feed samples. …”
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Dogs as sentinels for flavivirus exposure in urban, peri-urban and rural Hanoi, Vietnam by Long Pham-Thanh, Thang Nguyen-Tien, Magnusson, Ulf, Vuong Bui Nghia, Anh Bui-Ngoc, Duy Le-Thanh, Lundkvist, Å., Minh Can-Xuan, Thuy Nguyen-Thi Thu, Hau Vu-Thi Bich, Hu Suk Lee, Hung Nguyen-Viet, Lindahl, Johanna F.

“…A total of 475 dogs serum samples from 221 households in six districts of Hanoi were analyzed by a competitive enzyme-linked immunosorbent assay (cELISA) for antibodies to the pr-E protein of West Nile virus and other flaviviruses due to cross-reactivity. …”
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Seroprevalence and risk factors of Brucella infection in dairy animals in urban and rural areas of Bihar and Assam, India by Deka, Ram Pratim, Shome, R., Dohoo, I., Magnusson, Ulf, Grace, Delia, Lindahl, Johanna F.

“…Serological testing was conducted by indirect enzyme-linked immunosorbent assay (iELISA). Animal-level Brucella seropositivity was found to be 15.9% in Assam and 0.3% in Bihar. …”
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