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Characterization of distinct strains of an aphid-transmitted ilarvirus (Fam. Bromoviridae) infecting different hosts from South America por Silvestre, R., Fuentes, S., Risco, R., Berrocal, A., Adams, I., Fox, A., Cuéllar, Wilmer Jose, Kreuze, Jan F.

“…These isolates were identified using high throughput sequencing, ELISA, host range and RT-PCR. Here we report the sequence characterization of the complete genomes of nine PYV isolates found infecting Solanum tuberosum, four complete genome isolates infecting Smallanthus sonchifolius (yacon), and in addition 15 complete RNA3 sequences from potato and partial sequences of RNA1, 2 and 3 of isolates infecting potato and yacon from Ecuador, Peru and Bolivia. …”
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Metagenomic analysis of plant viruses associated with papaya ringspot disease in Carica papaya L. in Kenya por Mumo, N.N., Mamati, G.E., Ateka, E.M., Rimberia, F.K., Asudi, G.O., Boykin, L.M., Machuka, Eunice M., Njuguna, Joyce N., Pelle, Roger, Stomeo, Francesca

“…Previous attempts to detect PRSV in papaya plants showing PRSD symptoms, using enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR) procedures with primers specific to PRSV, have not yielded conclusive results. …”
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Japanese encephalitis in small-scale pig farming in rural Cambodia: Pig seroprevalence and farmer awareness por Henriksson, E., Söderberg, R., Ström Hallenberg, G., Kroesna, K., Ly, S., Sear, B., Unger, Fred, Tum, S., Hung Nguyen-Viet, Lindahl, Johanna F.

“…The sera were analysed with ELISA for JEV antibodies. Household representatives were interviewed, and data were recorded for each sampled pig. …”
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Co-infections of respiratory pathogens and gastro-intestinal parasites in smallholder pig production systems in Uganda por Oba, Peter, Wieland, Barbara, Mwiine, F.N., Erume, J., Dione, Michel M.

“…Sera were screened against 4 pathogens using commercial ELISA tests. The Baerman’s method was used to identify parasite species in faecal samples. …”
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Aflatoxin exposure among children of age 12-59 months in Butajira District, South-Central Ethiopia: A community based cross-sectional study por Ayele, M., Haile, D., Alonso, Silvia, Sime, H., Abera, A., Balcha, K.H., Roba, K.T., Guma, G.T., Endris, B.S.

“…Aflatoxin M1 concentration in urine was measured by Enzyme-Linked Immunosorbent assay (ELISA). The data analysis was carried out using STATA. …”
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Co-infection of pigs with Taenia solium cysticercosis and gastrointestinal parasites in Eastern and Western Uganda por Ngwili, Nicholas, Thomas, Lian F., Githigia, S., Muloi, Dishon M., Marshall, Karen, Wahome, R., Roesel, Kristina

“…Taenia spp. circulating antigen levels in the sample sera was tested using a commercial enzyme-linked immunosorbent assay kit, apDia™ cysticercosis Ag ELISA. The modified McMaster technique was used to identify and quantify the GI parasites. …”
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Diagnostic challenges of brucellosis in humans and livestock in Tanzania: A thematic review por Mengele, I.J., Shirima, G.M., Bronsvoort, B.M., Hernandez-Castro, L.E., Cook, Elizabeth A.J.

“…This study suggests that there is a need for (1) training on public health education and brucellosis awareness among stakeholders in the livestock value chain; (2) providing scheduled continuing professional education with regard to brucellosis and other zoonotic diseases to health and livestock workers; (3) future brucellosis surveillance studies must focus on unrepresented regions; and (4) lastly, we recommend that the rose Bengal plate test (RBPT) and competitive enzyme-linked immunosorbent assay (cELISA) should be considered in brucellosis diagnostic schemes as a complementary tool to hasten the implementation of an ongoing national strategy for the prevention and control of brucellosis in humans and livestock in Tanzania. …”
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Mapping the risk of Rift Valley fever in Uganda using national seroprevalence data from cattle, sheep and goats por Tumusiime, Dan, Isingoma, E., Tashoroora, O.B., Ndumu, D.B., Bahati, M., Nantima, N., Mugizi, Denis, Jost, C., Bett, Bernard K.

“…Serum samples collected were screened at the National Animal Disease Diagnostics and Epidemiology Centre (NADDEC) using a competition multispecies anti-RVF IgG ELISA kit. Data obtained were analyzed using a Bayesian model that utilizes integrated nested Laplace approximation (INLA) and stochastic partial differential equation (SPDE) approaches to estimate posterior distributions of model parameters, and account for spatial autocorrelation. …”
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Diagnostic Tools Applied by ICARDA’s Germplasm Health Unit (GHU) for Detection of Pests and Diseases Transmitted via Legume and Cereal Seeds por Kumari, Safaa, Moukahel, Abdulrahman, El Miziani, Inaam, Darwish, Iman

“…(i) visual inspection and floating test for stored insects, (ii) centrifuge washing, agar plate and freezing blotter tests for fungi, (iii) TBIA and ELISA for viruses, (iv) nematode extraction test for nematodes, and (v) agglutination test for bacteria. …”
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Genome characterization of Rift Valley fever virus isolated from cattle, goats and sheep during interepidemic periods in Kenya por Onwong'a, A.A., Oyola, Samuel O., Juma, John, Konongoi, S., Nyamota, Richard, Mwangi, Reuben, Muli, Collins, Dobi, Paul, Bett, Bernard K., Ongus, J.R.

“…The competitive IgM-capture ELISA, was used to screen the samples; 205 samples (29.67%) tested positive for RVFV. …”
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A retrospective investigation of abortion storm in Abergele goats, Waghimira Zone, Amhara Region, Ethiopia por Bahiru, A., Assefa, Ayalew, Gemeda, Biruk A., Desta, Hiwot, Tibebu, A., Sahle, A., Wieland, Barbara

“…The Rose Bengal Plate Test for Brucellosis, <i>Toxoplasma gondii</i> Antibody Test Kit for <i>Toxoplasma gondii</i>, and ELISA for <i>Chlamydophila abortus</i> and <i>Coxiella burnetii</i> were used to detect antibodies. …”
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Epidemiology of peste des petits ruminants in selected districts of Borena zone, Ethiopia por Kumbe, A., Negussie, H., Getachew, Y., Alemu, Biruk, Alemayehu, Gezahegn, Girma, S., Sibhatu, D., Emiyu, K., Waktole, H., Leta, S.

“…A total of 384 serum samples were collected randomly from sheep and goats and examined for the presence of PPRV antibodies using competition enzyme-linked immune sorbent assay (c-ELISA). Additionally, a retrospective analysis of five years of outbreak data was performed to provide insight into the spatial and temporal distribution of the disease. …”
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Sero-epidemiology of Coxiella burnetii in livestock and humans in Isiolo county Kenya por Mutisya, W.M., Akoko, James M., Mwatondo, Athman, Muturi, Mathew, Nthiwa, D., Abkallo, Hussein, Nyamota, Richard, Wachira, T., Gathura, P., Bett, Bernard K.

“…Indirect enzyme-linked immunosorbent assay (ELISA) was used to test the serum samples for antibodies against <i>C. burnetii</i>. …”
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Article 6.8 | CGIAR Road from COP29 to COP30Webinar Series #1 por CGIAR Science Program on Climate Action

“…This session will be moderated by CGIAR’s Eliza Villarino from the Alliance of Bioversity and CIAT.…”
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Attempts to protect goats against challenge with Trypanosoma vivax by initiation of primary infections with large numbers of metacyclic trypanosomes por Vos, G.J., Moloo, S.K., Nelson, R.T., Gardiner, Peter R.

“…All infected goats produced lytic antibodies to live bloodstream forms, as well as antibodies to bloodstream form lysates (demonstrated by ELISA). It is suggested that the immunity that had been induced in some of the experimental animals is due to antibody responses to both metacyclic and bloodstream variable antigen types (VATs) expressed during infection.…”
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Malaria vectors in a traditional dry zone village in Sri Lanka por Amerasinghe, Priyanie H., Amerasinghe, Felix P., Konradsen, Flemming, Fonseka, K.T., Wirtz, R.A.

“…Mosquito head-thoraces were tested for the presence of Plasmodium falciparum and P. vivax, and blood-engorged abdomens for the presence of human blood by ELISAs. House surveys were done at two-day intervals to record cases of blood film-confirmed malaria among the villagers. …”
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Use of “one-pot, mix-and-read” peptide-MHC class I tetramers and predictive algorithms to improve detection of cytotoxic T lymphocyte responses in cattle por Svitek, Nicholas, Hansen, A.M., Steinaa, Lucilla, Saya, R., Awino, Elias, Nielsen, Morten, Buus, Soren, Nene, Vishvanath M.

“…The algorithm NetMHCpan predicted alternative epitope sequences for some of the T. parva CTL epitopes. Using an ELISA assay to measure peptide-BoLA monomer formation and p-MHC class I tetramers of new specificity, we demonstrate that a predicted alternative epitope Tp229-37 rather than the previously reported Tp227-37 epitope is the correct Tp2 epitope presented by BoLA-6*04101. …”
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Enhancing resistance against African weevils through development of transgenic sweetpotato cultivars (Ipomoea batatas (L.) Lam.) expressing cry7Aa1, cry3Ca1 and ET33-34 genes. por Manrique Trujillo, S., Wamalwa, N.L., Díaz, D., Reano, R., Tovar, J.C., Prentice, K., Ormachea, M., Kreuze, Jan F., Machuka, J., Ghislain, M.

“…Transcriptional activity and Cry protein accumulation are being tested in leaves and storage roots by Real time PCR and DAS-ELISA respectively.…”
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Exposure of pigs to Trichinella spp. in three districts in Central and Eastern Uganda por Roesel, Kristina, Dione, Michel M., Nöckler, K., Fries, R., Baumann, M.P.O., Clausen, Peter-Henning, Grace, Delia

“…The presentation will discuss the implications of a sensitivity analysis, potential reasons for the high number of false–positives using the commercial ELISA as well as the suitability of indirect serological diagnostic tools developed in industrialized countries for utilization in extensive production systems in tropical countries.…”
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An initial characterization of aflatoxin B1 contamination of maize sold in the principal retail markets of Kigali, Rwanda por Nishimwe, Kizito, Wanjuki, I., Karangwa, C., Darnell, Ross, Harvey, Jagger J.W.

“…Enzyme-Linked Immunosorbent Assay (ELISA) and Immuno-affinity fluorimetery were used to analyze samples. …”
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