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Seroprevalence of brucellosis and risk factors associated with its seropositivity in cattle, goats and humans in Iganga District, Uganda por Nguna, J., Dione, Michel M., Apamaku, M., Majalija, S., Mugizi, R.D., Odoch, T., Kato, C.D., Tumwine, G., Kabaasa, J.D., Curtis, K., Graham, M., Ejobi, F., Graham, T.

“…Human, cattle, and goat blood samples were collected and tested serologically using commercial indirect-ELISA kits manufactured by USDA, USA. Results: of 451 human blood samples, 20 (4.4%) were positive. …”
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Zoonotic pathogen seroprevalence in cattle in a wildlife–livestock interface, Kenya por Nthiwa, D., Alonso, Silvia, Odongo, D., Kenya, E., Bett, Bernard K.

“…Sera samples were collected from 1170 cattle belonging to 390 herds in all the zones and tested for antibodies against Brucella abortus and Leptospira interrogans serovar hardjo using ELISA kits. Data on putative risk factors for seropositivity of these pathogens in cattle were collected using a questionnaire. …”
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Local ancestry to identify selection in response to trypanosome infection in Baoulé x Zebu crossbred cattle in Burkina Faso por Yougbaré, B., Ouédraogo, D., Tapsoba, A.S.R., Soudré, A., Zoma, B.L., Orozco-terWengel, P., Moumouni, S., Ouédraogo-Koné, S., Wurzinger, Maria, Tamboura, H.H., Traoré, A., Okeyo Mwai, Ally, Sölkner, J., Khayatzadeh, N., Mészáros, Gábor, Burger, P.A.

“…Using a medium-density genomic marker panel from the Illumina Bovine SNP50 BeadChip, we estimated individual admixture proportions for Baoulé x Zebu crossbred cattle in Burkina Faso, which were tested for trypanosome infection by direct ELISA from blood samples. Furthermore, we calculated local ancestry deviation from average for each SNP across 29 autosomes to identify potential regions under selection in the trypanotolerant Baoulé cattle and their crossbreds. …”
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Seropositivity rates of zoonotic pathogens in small ruminants and associated public health risks at informal urban markets in Zambia por Lysholm, S., Fischer, K., Lindahl, Johanna F., Munyeme, M., Wensman, Jonas Johansson

“…In April, May and September 2018, serum samples (n = 237) were collected and analysed for antibodies for the zoonotic pathogens Brucella spp., Coxiella (C.) burnetii and Rift Valley fever virus (RVFV), using commercially available enzyme linked immunosorbent assays (ELISA). In addition, slaughterhouse activities were observed and semi-structured interviews and focus group discussions held with slaughterhouse workers and small ruminant traders, focusing on the handling of animals and meat, and the perceptions of zoonotic disease risks at slaughter and consumption. …”
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Seroprevalence and associated factors of trichinellosis in indigenous pigs and rural communities in Northern Vietnam por Sinh Dang-Xuan, Nga Vu-Thi, Dung Do-Trung, Trang Le-Thi-Huyen, Ngoc Pham Thi, Unger, Fred

“…A total of 1,000 serum samples (500 indigenous pigs and 500 community participants) was collected and tested using a commercial ELISA test kit (Priocheck, ThermoFisher). Structure questionnaires were also used to interview selected pig owners and sampled community members regarding pig raising practice, eating behavior and disease knowledge and prevention. …”
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Early-stage phenotyping of sweet potato virus disease caused by sweet potato chlorotic stunt virus and sweet potato virus C to support breeding por David, M., Kante, M., Fuentes, S., Eyzaguirre, R., Diaz, F., Boeck, B. de, Mwanga, Robert O.M., Kreuze, Jan F., Grüneberg, W.J.

“…The first part of the protocol was based on ELISA results for sweet potato chlorotic stunt virus (SPCSV) and sweet potato virus C (SPVC) with adjustments to a negative control (uninfected clone 'Tanzania') and was performed on a pre-breeding population (VZ08) comprising 455 clones and 27 check clones graft-inoculated under screenhouse conditions. …”
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High-throughput characterization and phenotyping of resistance and tolerance to virus infection in sweetpotato por Kreuze, Jan F., Ramírez, D., Fuentes, S., Loayza, H., Ninanya, J., Rinza, J., David, M., Gamboa, S., Boeck, B. de, Díaz, F., Pérez, A., Silva, L., Campos, Hugo

“…Virus loads were assessed using ELISA, (RT-)qPCR, and loop-mediated isothermal amplification (LAMP) methods, and also through multispectral reflectance and canopy temperature collected using an unmanned aerial vehicle. …”
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Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Bean yellow mosaic virus (BYMV) in legumes applied by ICARDA’s GHU por Kumari, Safaa, Moukahel, Abdulrahman

“…This hybrid diagnostic technique is more sensitive than ELISA and RT-PCR alone. In addition, Immunocapture of virus particles step provides a simple method to isolate virus particles from plant tissue, particularly when inhibitory substances are present, and thus enables subsequent use of RT-PCR amplification. …”
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African swine fever virus rapid detection assays evaluation por Lacasta, Anna, Oba, Peter, Dione, Michel M.

“…They include Enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody (IFA) test and immunoblotting. …”
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Seroprevalence of brucellosis, knowledge, and risky practices in dairy cattle owners and workers in Maekel and Debub regions, Eritrea por Efrem, G.H., Mihreteab, B., Ghebremariam, M.K., Tesfai, B., Mor, Siobhan M., Mamo, G.

“…Blood samples were tested using Rose Ben gal Plate Test, and positive samples were confirmed using competitive ELISA. Variation in knowledge scores by sociode mographic factors and practices were explored statistically. …”
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African swine fever virus antibody rapid detection assays evaluation: Evaluation of rapid lateral flow assays for the detection of antibodies against African swine fever virus for... por Lacasta, Anna, Oba, Peter, Dione, Michel M.

“…They include enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody test and immunoblotting. …”
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Comparison of African swine fever virus prevalence and risk in two contrasting pig-farming systems in South-west and Central Kenya por Okoth, Edward A., Gallardo, C., Macharia, Joseph M., Omore, Amos O., Pelayo, V., Bulimo, D.W., Arias, Marisa, Kitala, P., Baboon, K., Lekolol, I., Mijele, D., Bishop, Richard P.

“…No animals were sero-positive in either study site using the OIE indirect-ELISA and none of the animals sampled exhibited clinical symptoms of ASF. …”
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African swine fever viruses with two different genotypes, both of which occur in domestic pigs, are associated with ticks and adult warthogs, respectively, at a single geographical... por Gallardo, C., Okoth, S., Pelayo, V., Anchuelo, R., Martin, E., Simon, A., Llorente, A., Nieto, R., Soler Garzón, Alvaro, Martin, R., Arias, Marisa, Bishop, Richard P.

“…All of the warthog sera were positive for anti-ASFV antibodies, investigated by using ELISA, but none of the domestic pig sera were positive. …”
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A survey of bovine cysticercosis/human taeniosis in Northern Turkana District, Kenya por Asaava, L.L., Kitala, P.M., Gathura, P.B., Nanyingi, M.O., Muchemi, G., Schelling, E.

“…The seroprevalence of cysticercosis in cattle was estimated at 16.7% (95% CI 13–20.9%) using a secretory–excretory antigen detection ELISA. There was poor agreement between meat inspection and serology (k = 0.025; p = 0.2797). …”
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Effect of trypanosomosis on the resumption of postpartum ovarian function and oestrus activity in Zebu cows por Tibbo, Markos, Mukasa-Mugerwa, E., Abebe, G., Mulatu, W.

“…This was done through the determination of milk progesterone levels using the enzyme linked immunosorbent assay (ELISA), packed cell volume (PCV), and darkground/phase contrast buffy coat examination to estimate the parasitic burden. …”
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Exposure to aflatoxin B1 in utero is associated with DNA methylation in white blood cells of infants in The Gambia por Hernández Vargas, H., Castelino, J., Silver, M.J., Domínguez Salas, Paula, Cros, M.P., Durand, G., Calvez-Kelm, F. Le, Prentice, A.M., Wild, C.P., Moore, S.E., Hennig, B.J., Herceg, Z., Yun Yun Gong, Routledge, M.N.

“…Aflatoxin exposure in women was assessed using an ELISA method to measure aflatoxin albumin (AF-alb) adducts in plasma taken at 1–16 weeks of pregnancy. …”
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Prevalence of aflatoxin contamination in cereals from Nandi County, Kenya por Sirma, A.J., Ouko, E.O., Murithi, G., Mburugu, C., Mapenay, I., Ombui, J.N., Kang'ethe, Erastus K., Korhonen, H.

“…The samples were tested for total aflatoxin contamination using cELISA. Households in the study sub-locations depended on homegrown grains than market sourced. …”
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Antibiotic residues and heavy metals in pork at wet markets in Vietnam por Tran Thi Tuyet Hanh, Sinh Dang Xuan, Hung Nguyen-Viet, Unger, Fred, Chu Van Tuat, Ngan Tran Thi, Phuc Pham Duc

“…Tetracycline, fluoroquinolones, sulfonamide, chloramphenicol groups were firstly screened by ELISA and positive samples were quantified by liquid chromatography–mass spectrometry/mass spectrometry (LCMS/MS). …”
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The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes por Bishop, Richard P., Hemmink, W.I., Morrison, J.D., Weir, W., Toye, Philip G., Sitt, Tatjana, Spooner, P.R., Musoke, A.J., Skilton, Robert A., Odongo, David O.

“…(buffalo), which is genetically very closely related to T. parva, according to 18s rDNA sequence, has a conserved orthologue of the polymorphic immunodominant molecule (PIM) that forms the basis of the diagnostic ELISA used for T. parva serological detection. Closely related orthologues of several CD8 T cell target antigen genes are also shared with T. parva. …”
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Incidence and diversity of mixed viruses lower in yam tubers and tuber sprouts compared with field leaf samples: Implications for virusfree planting material control strategy por Eni, A., Hughes, J., Asiedu, Robert, Rey, M.

“…Leaf samples from the tuber sprouts were tested by ELISA and/or IC-PCR/IC-RT-PCR to determine the incidence of Yam mosaic virus, Yam mild mosaic virus, cucumber mosaic virus, and Dioscorea Bacilliform viruses. …”
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