Search Results - Épila~

Survey on cowpea virus incidence and distribution in Nigeria by Shoyinka, S.A., Thottappilly, G., Adebayo, G.G., Anno-Nyako, F.O.

“…Enzyme-linked immunosorbent assay (ELISA) was used to detectviruses in649 cowpea leafsamples collectedfrom115 fields during a 3-year survey carried out from 1991 to 1993 throughout all agroecological zones in Nigeria. …”
Get full text

Incidence of Garlic common latent virus in Argentina, and phylogenetic and recombination analyses of isolates = Incidência de Garlic common latent virus na Argentina, e análise fi... by Torrico Ramallo, Ada Karina, Celli, Marcos Giovani, Conci, Luis Rogelio, Conci, Vilma Cecilia

“…Virus infection was evaluated with DAS‑Elisa test using specific antiserum, and the phylogenetic and recombination analyses were done with capsid protein (CP) nucleotide sequence of seven GarCLV isolates from the provinces. …”
Get full text
Get full text
Get full text

Advances in the etiology of sweet potato (Ipomoea batatas (L.) Lam) yellow curling disease in Argentina by Flamarique, Sofía Solange, Vilanova Perez, Antonella, Luque, Andres Vicente, Rodriguez Pardina, Patricia, Di Feo, Liliana Del Valle

“…The presence of each virus was detected by serological (DAS, NCM and TAS-ELISA) and molecular (PCR) tests. It is concluded that the interaction of SPFMV, SPVG, SPCSV and SPLCV is needed for the development of YC symptoms.…”
Get full text
Get full text

Humoral immune response of pregnant goats to two Corynebacterium pseudotuberculosis bacterin formulations by Auad, Jordana, Cerutti, Julieta, Cooper, Laura Gabriela, Camussone, Cecilia, Lozano, Natalia A., Crespo, Francisco M., Lozano, Alejandro

“…Antibodies (IgG) against this antigen were measured by ELISA. A robust humoral immune response was detected in vaccinated animals, with production of specific IgG. …”
Get full text
Get full text
Get full text

Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies by Bellido, Demian, Chacana, Pablo, Mozgovoj, Marina Valeria, Gonzalez, Diego, Goldbaum, Fernando Alberto, Wigdorovitz, Andres, Dus Santos, Maria Jose

“…Hens inoculated with BLSVP8d developed higher antibody titers (evaluated by ELISA and viral neutralization test) than hens immunized either with VP8d alone or the mixture of VP8d and BLS. …”
Get full text
Get full text
Get full text

The first caprine rotavirus detected in Argentina displays genomic features resembling virus strains infecting members of the Bovidae and Camelidae by Louge Uriarte, Enrique Leopoldo, Badaracco, Alejandra, Matthijnssens, Jelle, Zeller, Mark, Heylen, Elizabeth, Manazza, Jorge Alberto, Miño, Samuel, Ranst, Marc Van, Odeon, Anselmo Carlos, Parreño, Gladys Viviana

“…Four out of twenty fecal samples from diarrheic and non-diarrheic goat kids were positive for RVA by ELISA. A representative sample was selected for further genome analyses. …”
Get full text
Get full text
Get full text

Immune response to Neospora caninum live tachyzoites in prepubertal female calves by Hecker, Yanina, Regidor-Cerrillo, Javier, Fiorani, Franco, Horcajo, Pilar, Soria, Ivana, Gual, Ignacio, Torioni, Susana Marta, Campero, Lucia, Echaide, Ignacio Eduardo, Alvarez-García, Gema, Ortega-Mora, Luis, Zamorano, Patricia Ines, Venturini, María Clara, Odeon, Anselmo Carlos, Canton, German Jose, Moore, Prando Dadin

“…Although chronic N. caninum infection was developed in 50% of calves of group A after inoculation, according to the presence of antibodies against rNc-SAG4, antigen characteristic of bradyzoites, N. caninum antibodies dropped below the cut-off of ELISA from day 210 post-inoculation onwards. Future trials using the same group of inoculated animals will allow the characterization of the evolution of the immune response during pregnancy and to determine whether the immunization with the local isolate is able to prevent congenital transmission and to protect against heterologous challenges.…”
Get full text
Get full text
Get full text

Spiraea sp. new natural host of Plum pox virus (Sharka) by Piglionico, Damián Gustavo, Ojeda, Eva Maria, Lucero, Vanesa Angelica, Farrando, Roberto Jes, Porcel, Laura Beatriz, Picca, Cecilia Nelida, Marini, Diana Beatriz

“…Symptomatic leaves were analyzed for PPV by DAS-ELISA with positive results. The presence of the virus was confirmed by conventional and qRT-PCR and the use of “GF-305” biological indicator plants inoculated by T-bud grafting with buds from the Spiraea. …”
Get full text
Get full text
Get full text

Production of a monoclonal antibody specific to the El Amar strain of plum pox virus by Myrta, A., Potere, O., Boscia, D., Candresse, Thierry, Cambra, Mariano, Savino, V.

“…After a preliminary characterization by double-antibody sandwich enzyme-linked immunosorbent assay(DAS-ELISA), 1 of 13 selected MAbs proved to be EA strain-specific. …”
Get full text

Real-time assay for quantitative detection of non-persistently transmitted Plum pox virus RNA targets in single aphids by Olmos, Antonio, Bertolini, Edson, Gil, Maite, Cambra, Mariano

“…The number of viruliferous aphids detected by real-time RT-PCR and nested RT-PCR in a single closed tube was similar in parallel assays, nevertheless the sensitivity provided by real-time RT-PCR was 100 times higher than nested RT-PCR and 1000 times higher than DASI-ELISA and conventional RT-PCR. The quantities of PPV-RNA targets detected in a single aphid ranged from 40 to more than 2 x 10(3) units. …”
Get full text

Efficient production of transgenic citrus plants expressing the coat protein gene of citrus tristeza virus by Domínguez, Alfons, Guerri, José, Cambra, Mariano, Navarro, Luis, Moreno, Pedro, Pena, Leandro

“…Expression of the CTV-CP transgene was demonstrated in 38 out of 42 plants by western analysis and DASI-ELISA. No correlation was found between coat protein expression and transgene copy number or integration pattern.…”
Get full text

Differentiation of citrus tristeza virus isolates by serological analysis of p25 coat protein peptide maps by Albiach-Martí, María R., Guerri, José, Cambra, Mariano, Garnsey, Stephen M., Moreno, Pedro

“…Some of these isolates had been indistinguishable based on their reactivity in DASI-ELISA, dsRNA pattern and biological characterization. …”
Get full text

A New Graft Transmissible Disease Found in Nagami Kumquat by Navarro, Luis, Pina, José A., Ballester-Olmos, José F., Moreno, Pedro, Cambra, Mariano

“…Diseased kumquats gave negative reactions by ELISA using four different tristeza antisera.…”
Get full text
Get full text

Osteoarthritis : the inflammatory reaction and medical treatment by Andersson, Anna-Maria

“…In this pilot study of five horses, an ELISA was used to measure TNFα in synovial fluid from fetlock joints. …”

Prevalens av Sarcoptes scabiei hos thailändska gatuhundar by Fant, Daniel

“…Blood samples were collected to determine the seroprevalences of IgG-antibodies against S. scabiei with ELISA. From 57 of the 62 dogs, two skin scrapings each were collected. …”
Get full text

Prevalens av subkliniskt smittade katter med vingelsjuka i Göteborg jämfört med Uppsala by Eriksson, Jonas

“…Blood samples were collected in Gothenburg and Uppsala to study the prevalence of antibodies against BDV in healthy, outdoor cats that were more than one year of age. Realtime-PCR and ELISA-studies were performed. More studies are needed to make the diagnostics to work and to be able to study the prevalence of BDV in cats outside the region of Mälardalen.…”
Get full text

Infection of rotavirus in dairy calves in south Vietnam by Kyle, Helena

“…The samples were analysed for presence of rotavirus A by ELISA. 18 samples were positive for rotavirus, 2 of these samples were co-infections with C. parvum. 17 of the positive samples were found in state farms. …”
Get full text

Neospora caninum hos köttdjur i Sverige by Westin, Lena

“…caninum specific antibodies by ELISA. Thirtyfive of 986 (3.6%) animals had antibodies to the parasite. …”
Get full text

To change everything without changing everything : applying Martí Franch’s differentiated management methods to Nytorps gärde by Richold, Jack

“…Metoden använder först och främst enkla skötselmetoder som klippning och hamling för att skapa attraktiva, tillgängliga landskap som är billiga att implementera och utveckla. …”

Detection of adenoviruses in cattle by Mamadatokhonova, Guldasta

“…In conclusion, the TaqMan real-time PCR assay presented here is expected to be a good alternative tool to the traditional diagnostic methods like virus isolation, ELISA or conventional PCR in case of sporadic disease outbreak in which rapid, sensitive and specific diagnosis is required and when appropriate measures should be taken.…”
Get full text