Resultados de búsqueda - "nucleic acid"

  1. A New Indexing Method for Cachexia por Durán-Vila, Núria, Pina, José A., Molins, M., Navarro, Luis

    Publicado 2022
    “…The utilization of nucleic acid extraction and polyacrylamide gel electrophoresis as an alternative to the Parson's Special bioassay for routine indexing of cachexia was evaluated. …”
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  2. Isolation and partial characterization of a geminivirus causing bean dwarf mosaic por Morales, F, Niessen, A, Ramírez, B., Castaño, M.

    Publicado 1990
    “…Dissociated infectious nucleoprotein yielded a single protein species of molecular mass 27,500 daltons and a single nucleic acid band of approximately 2.6 kb. Purified nucleic acid was resistant to RNase but sensitive to DNase 1 and nuclease S1, indicating the presence of single-stranded DNA. …”
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    Journal Article
  3. A novel RT-PCR approach for detection and characterization of citrus viroids por Bernad, Lucia, Durán-Vila, Núria

    Publicado 2017
    “…The Suitability of the technique has been demonstrated using several nucleic acid extraction procedures and different viroid infected host species. …”
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  4. Host effect on the molecular and biological properties of a Citrus exocortis viroid isolate from Vicia faba por Gandía, Mónica, Bernad, Lucia, Rubio, Luis, Durán-Vila, Núria

    Publicado 2017
    “…However, when nucleic acid extracts from this tomato isolate were back inoculated to new broad bean plants, this isolate did not revert to the original population, because it showed low nucleotide diversity (0.001) and induced symptoms in the broad bean plants. …”
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  5. Methods for the detection of plant viruse diseases por Naidu, R., Hughes, J.

    Publicado 2003
    “…Viral nucleic acid-based techniques like dot-blot hybridization assays and polymerase chain reaction are more sensitive than other methods. …”
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    Conference Paper
  6. Guidelines for coconut germplasm movement amended por Technical Centre for Agricultural and Rural Cooperation

    Publicado 1998
    “…As part of research into the damaging disease in coconuts caused by the cadang cadang viroid, it was noticed that Pacific coconuts had viroid-like nucleic acid sequences present in their tissues, although this appeared to be unassociated with...…”
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    News Item
  7. A closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis por Trangoni, Marcos David, Gioffre, Andrea, Cravero, Silvio Lorenzo Pedro

    Publicado 2020
    “…Despite the conceptual complexity underlying the mechanistic basis for the nucleic acid amplification, the technique is simple to use and the amplification and detection can be carried out in just one step. …”
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  8. Biological and Molecular Characterization of Two Isolates of Citrus Viroids Recovered from Cuban Plantations por Velázquez, Karelia, Soto, Marianella, Perez, Rosa, Rodríguez, D., Durán-Vila, Núria

    Publicado 2021
    “…The nucleic acid extracts were subjected to retrotranscription, PCR amplification, cloning, SSCP analysis and sequencing. …”
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  9. Are molecular tools solving the challenges posed by detection of plant pathogenic bacteria and viruses? por López, María M., Llop, Pablo, Olmos, Antonio, Marco-Noales, Ester, Cambra, Mariano, Bertolini, Edson

    Publicado 2017
    “…Nucleic acid extraction is still necessary in many cases and in practice inhibition problems are decreasing the theoretical sensitivity of molecular detection. …”
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  10. Incidence, distribution and first identification of citrus tristeza virus by RTPCR in citrus orchards in southWestern Nigeria por Adediji, A.O., Atiri, G.I., Kumar, P. Lava

    Publicado 2015
    “…Symptoms observed included stem pitting, stunting, vein clearing and leaf curling. Nucleic acid extracts from leaves were used to detect CTV by RT-PCR using primers PIN1 and PIN2 directed to the conserved 3’ untranslated region. …”
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    Journal Article
  11. Identification of okra mosaic virus from Indigofera spicata in Nigeria por Thottappilly, G.

    Publicado 1996
    “…Its identity was established on the basis of particle morphology, analysis of viral coat protein and nucleic acid and Indigofera spicata. In reciprocal agar gel diffusion tests, the virus isolate from I. spicata and an OMV isolate from okra in Ibadan (OMV-Ibadan isolate) were found to be serologically identical. …”
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    Journal Article
  12. Application of genetic markers to forest tree species: Draft report to IPGRI of the project Developing Decision-making Strategies on priorities for conservation and use of forest g... por Namkoong, G., Koshy, M.P.

    Publicado 2001
    “…The application of genetic markers such as allozymes and DNA (Deoxyribose Nucleic Acid) markers has shown to be useful in studying genetic diversity in humans, animals and plants (Cruzan, 1998). …”
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    Otro
  13. Modeling the Accuracy of Three Detection Methods of Grapevine leafroll-associated virus 3 During the Dormant Period Using a Bayesian Approach por Olmos, Antonio, Bertolini, Edson, Ruiz-García, Ana B., Martínez, M. Carmen, Peiró, Rosa, Vidal, Eduardo

    Publicado 2020
    “…Although different serological and nucleic acid-based methods have been developed for the detection of GLRaV-3, diagnostic parameters have not been established, and there is no gold standard method. …”
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