Resultados de búsqueda - "genome sequencing"

Design of an in-silico workflow to trace the spread of zoonotic bacteria using NGS data por Janssens, Adrien

“…In this work, we will discuss how bioinformatic tools, and in particular SNV calling and tree-building softwares, can be used to create a single-command, easy to use, fast-processing workflow using genomic sequencing data to analyze trace of bacteria from different locations and times; and how these results can be interpreted and exploited. …”
Enlace del recurso

Salmonella identified in pigs in Kenya and Malawi reveals the potential for zoonotic transmission in emerging pork markets por Wilson, C.N., Pulford, C.V., Akoko, James M., Sepulveda, B.P., Predeus, A.V., Bevington, J., Duncan, P., Hall, N., Wigley, P., Feasey, N., Pinchbeck, G., Hinton, J.C.D., Gordon, M.A., Fèvre, Eric M.

“…Selective culture, antisera testing and whole genome sequencing were performed on samples from 647 pigs; the prevalence of NTS carriage was 12.7% in Busia, 9.1% in Nairobi and 24.6% in Chikwawa. …”
Enlace del recurso

Genetic structure of Arabian Peninsula dromedary camels revealed three geographic groups por Almathen, F., Bahbahani, H., Elbir, H., Alfattah, M., Sheikh, A., Hanotte, Olivier H.

“…The results obtained here need to be further validated using whole genome sequencing data.…”
Enlace del recurso

Genome-wide association analysis uncovers rice blast resistance alleles of Ptr and Pia por Greenwood, Julian R., Lacorte-Apostol, Vanica, Kroj, Thomas, Padilla, Jonas, Telebanco-Yanoria, Mary Jeanie, Glaus, Anna N., Roulin, Anne, Padilla, André, Zhou, Bo, Keller, Beat, Krattinger, Simon G.

“…Our work shows that GWAS in combination with whole-genome sequencing is a powerful tool for gene cloning and to facilitate selection of specific resistance alleles for plant breeding.…”
Enlace del recurso

Status and prospects of biotechnology applications to conservation, propagation and genetic improvement of yam por Balogun, M., Gueye, B.

“…Marker-assisted selection, embryo culture, genetic transformation and genome sequencing have been initiated to support conventional genetic improvement, but investigations into the presence of transposable elements, site targeted mutagenesis, somatic embryogenesis and haploid plant production will be necessary to fast track the genetic improvement. …”
Enlace del recurso

Functional Genomics in Fruit Trees: From 'Omics to Sustainable Biotechnologies por Licciardello, Concetta, Perrone, Irene, Gambino, Giorgio, Talón, Manuel, Velasco, Riccardo

“…Moreover, the Research Topic illustrates a technological application path from whole-genome sequencing (Wu et al.) to the resequencing (Tanaka et al.), to achieve the ultimate objective of modulating genes using the New Breeding Techniques (Salonia et al.).…”
Enlace del recurso
Enlace del recurso

The effect of different antimicrobial treatment regimens on the faecal shedding of ESBL-producing Escherichia coli in horses por Schoster, A., Spijk, J.N. van, Damborg, P., Moodley, Arshnee, Kirchgaessner, C., Hartnack, S., Schmitt, S.

“…On a subset of ESBL-EC isolates, antimicrobial susceptibility testing (n = 45) and whole-genome sequencing followed by SNP-analysis (n = 46) were performed. …”
Enlace del recurso

Genome Wide Identification of the MLO Gene Family Associated with Powdery Mildew Resistance in Rubber Trees (Hevea brasiliensis) por Liyanage, K.K., Khan, S., Herath, V., Brooks, S., Mortimer, P.E., Nadir, S., Hyde, Kevin D., Xu, J.C.

“…In this study, we describe the whole-genome sequencing of a PM resistant rubber clone, RRIC 52, as well as a PM susceptible clone, PB 235, using massively parallel paired-end sequencing. …”
Enlace del recurso

Large genomic introgression blocks of Phaseolus parvifolius Freytag bean into the common bean enhance the crossability between tepary and common beans por Barrera, Santos, Berny Mier y Teran, Jorge Carlos, Lobaton, Juan David, Escobar Pérez, Roosevelt Humberto, Gepts, Paul L., Beebe, Stephen E., Urrea, Carlos A.

“…Crossing these three interspecific lines with tepary beans resulted in 12-fold more hybrid plants than crossing traditional common beans with tepary beans. Whole-genome sequencing analysis of these three interspecific lines shows large introgressions of genomic regions corresponding to P. parvifolius on chromosomes that presumably contribute to reproductive barriers between both species. …”
Enlace del recurso

Absolute Quantification of the Host-To-Parasite DNA Ratio in Theileria parva-Infected Lymphocyte Cell Lines por Gotia, Hanzel T., Munro, James B., Knowles, Donald P., Daubenberger, Claudia A., Bishop, Richard P., Silva, Joana C.

“…Knowledge of the proportion of target DNA in a sample, as enabled by this method, is essential for efficient high-throughput genome sequencing applications for a variety of intracellular pathogens. …”
Enlace del recurso

Integrated community-based reporting and field diagnostics for improved rabies surveillance in rural Laikipia, Kenya por Odinga, Christian O., Thomas, Lian F., Wambugu, E., Ferguson, A.W., Fèvre, Eric M., Gibson, A., Hassell, James M., Muloi, Dishon M., Murray, S., Surmat, A., Mwai, P.M., Woodroffe, R., Ngatia, D., Gathura, P.M., Waitumbi, J., Worsley-Tonks, Katherine E.L.

“…Two positive samples processed successfully for whole genome sequencing indicated that the rabies variant circulating in central Kenya during the study period belonged to the Africa 1b subclade, which is similar to variants identified in eastern Kenya and Tanzania, suggesting regional transmission. …”
Enlace del recurso

Utilizing PacBio Iso-Seq for novel transcript and gene discovery of abiotic stress responses in Oryza sativa L. por Schaarschmidt, Stephanie, Fischer, Axel, Lawas, Lovely Mae F., Alam, Rejbana, Septiningsih, Endang M., Bailey-Serres, Julia, Jagadish, Krishna S.V., Huettel, Bruno, Hincha, Dirk K., Zuther, Ellen

“…In addition, our approach provides a cost-effective alternative to genome sequencing for identification of candidate genes in highly stress tolerant genotypes.…”
Enlace del recurso

An xa5 resistance gene-breaking indian strain of the rice bacterial blight pathogen Xanthomonas oryzae pv. oryzae is nearly identical to a Thai strain por Carpenter, Sara C. D., Mishra, Prashant, Ghoshal, Chandrika, Dash, Prasanta K., Wang, Li, Midha, Samriti, Laha, Gouri S., Lore, Jagjeet S., Kositratana, Wichai, Singh, Nagendra K., Singh, Kuldeep, Patil, Prabhu B., Oliva, Ricardo, Patarapuwadol, Sujin, Bogdanove, Adam J., Rai, Rhitu

“…The findings suggest international migration of the xa5-compatible pathotype and highlight the utility of whole genome sequencing and TALE analysis for understanding and responding to breakdown of resistance.…”
Enlace del recurso

Diverse genetic mechanisms underlie worldwide convergent rice feralization por Qiu, Jie, Jia, Lei, Wu, Dongya, Weng, Xifang, Chen, Lijuan, Sun, Jian, Chen, Meihong, Mao, Lingfeng, Jiang, Bowen, Ye, Chuyu, Turra, Guilherme Menegol, Guo, Longbiao, Ye, Guoyou, Zhu, Qian-Hao, Imaizumi, Toshiyuki, Song, Beng-Kah, Scarabel, Laura, Merotto, Aldo, Olsen, Kenneth M., Fan, Longjiang

“…Results We use whole-genome sequencing to examine the origin and adaptation of 524 global weedy rice samples representing all major regions of rice cultivation. …”
Enlace del recurso

Exploiting genetic and genomic resources to enhance productivity and abiotic stress adaptation of underutilized pulses por Dwivedi, S.L., Chapman, M.A., Abberton, M., Akpojotor, U.L., Ortiz, R.

“…This review article considers 13 underutilized pulses and focuses on their germplasm holdings, diversity, crop-wild-crop gene flow, genome sequencing, syntenic relationships, the potential for breeding and transgenic manipulation, and the genetics of agronomic and stress tolerance traits. …”
Enlace del recurso

Circulation of Salmonella spp. between humans, animals and the environment in animal-owning households in Malawi por Wilson, C.N., Musicha, P., Beale, M.A., Diness, Y., Kanjerwa, O., Salifu, C., Katuah, Z., Duncan, P., Nyangu, J., Mungu, A., Deleza, M., Banda, L., Makhaza, L., Elviss, N., Jewell, C.P., Pinchbeck, G., Feasey, N.A., Fèvre, Eric M., Thomson, N.R.

“…Between November 2018 and December 2019, we isolated <i>Salmonella</i> spp. from thirty households in urban and rural locations in Malawi, sampling at three time points from the stool of humans, animals, and their household environment. Using whole genome sequencing and fine-resolution bioinformatic and phylogenetic analyses we found evidence of sharing of <i>Salmonella</i> species and strains between humans, animals and the environment, both within and between households. …”
Enlace del recurso

perspectives on the application of next generation sequencing to the improvement of Africa's staple food crops por Gedil, Melaku A, Ferguson, Morag E., Girma Tessema, G., Gisel, A., Stavolone, L., Rabbi, Ismail Y.

“…NGS enables whole-genome sequencing (WGS) and re-sequencing, transcriptome sequencing, metagenomics, as well as high-throughput genotyping, which can be applied for genome selection (GS). …”
Enlace del recurso

Deep assessment of genomic diversity in cassava for herbicide tolerance and starch biosynthesis por Duitama, Jorge, Kafuri, Lina, Tello, Daniel, Leiva Sandoval, Ana Maria, Hofinger, Bernhard, Datta, Sneha, Lentini, Zaida, Aranzales Rondón, Ericson, Till, Bradley, Ceballos, H.

“…Targeted resequencing, either by pooled samples or by similar approaches such as Ecotilling or capture, emerges as a cost effective alternative to whole genome sequencing to identify interesting alleles of genes related to relevant traits within large germplasm collections.…”
Enlace del recurso

Resequencing of common bean identifies regions of inter- gene pool introgression and provides comprehensive resources for molecular breeding por Lobaton, Juan David, Miller, Tamara, Gil, Juanita, Ariza, Daniel, Hoz, Juan Fernando de la, Soler Garzón, Alvaro, Beebe, Stephen E., Duitama, Jorge, Gepts, Paul L., Raatz, Bodo

“…Gray), and Phaseolus coccineus L. were sequenced by whole-genome sequencing, identifying more than 40 million genomic variants. …”
Enlace del recurso

Data from: Resequencing of common bean identifies regions of inter-gene pool introgression and provides comprehensive resources for molecular breeding por Lobaton, Juan David, Miller, Tamara, Gil, Juanita, Ariza, Daniel, Hoz, Juan Fernando de la, Soler Garzón, Alvaro, Beebe, Stephen E., Duitama, Jorge, Gepts, Paul L., Raatz, Bodo

“…For this, 37 varieties belonging to P. vulgaris, P. acutifolius, and P. coccineus were sequenced by whole-genome sequencing (WGS), identifying more than 40 million genomic variants. …”
Enlace del recurso