Resultados de búsqueda - "engineer"

  1. Diseño de un sistema de anclaje para un tractor con malacate. por Rincón C., Carlos J.

    Publicado 2019
    “…This project deals with the design of a winch sprag for the Spider tractor developed at tha National College of Agricultural Engineering in an attempt to provide a cheap source of mechanical power for small farmers of developing countries. …”
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  2. From the smallest to the largest subcellular plant pathogen: Citrus tristeza virus and its unique p23 protein por Moreno, Pedro, Lopez, Carmelo, Ruiz-Ruiz, Susana, Pena, Leandro, Guerri, José

    Publicado 2022
    “…Professor Ricardo Flores substantially contributed to these advances in topics like: I) improvement of virus purification to obtain biologically active virions, II) sequencing mild CTV isolates for genetic comparisons with sequences of moderate or severe isolates and genetic engineering, III) analysis of genetic variation of both CTV genomic RNA ends and features of the highly variable 5′ end that allow accommodating this variation within a conserved secondary structure, IV) studies on the structure, subcellular localization and biological functions of the CTV-unique p23 protein, and v) potential use of p23 and other 3′ -proximal regions of the CTV genome to develop transgenic citrus resistant to the virus. …”
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  3. Plum pox virus silencing of C5 transgenic plums is stable under challenge inoculation with heterologous viruses por Zagrai, Luminita Antonela, Capote, Nieves, Ravelonandro, M., Cambra, Mariano, Zagrai, L., Scorza, Ralph

    Publicado 2017
    “…Virus infections were evaluated by visual monitoring of symptom and by serological and molecular assays. The engineered resistance to PPV in C5 transgenic plums was stable and was not suppressed by the presence of the challenging heterologous viruses over a three-year experimental period across all trials.…”
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  4. Construction of Agrobacterium tumefaciens-mediated tomato black ring virus infectious cDNA clones por Zarzyńska-Nowak, Aleksandra, Ferriol, Inmaculada, Falk, Bryce W., Borodynko-Filas, Natasza, Hasiów-Jaroszewska, Beata

    Publicado 2020
    “…Here, a successful construction of infectious full-length cDNA clones of the TBRV genomic RNAs (RNA1 and RNA2) is reported for the first time. The engineered constructs consisting of PCR-amplified DNAs were cloned into binary vector pJL89 immediately downstream of a double cauliflower mosaic virus (CaMV) 35S promoter, and upstream of the hepatitis delta virus (HDV) ribozyme and nopaline synthase terminator (NOS). …”
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  5. Microevolution rather than large genome divergence determines the effectiveness of legume–rhizobia symbiotic interaction under field conditions por Jozefkowicz, Cintia, Brambilla, Silvina Maricel, Frare, Romina Alejandra, Stritzler, Margarita, Puente, Mariana Laura, Piccinetti, Carlos Fabian, Soto, Gabriela Cinthia, Ayub, Nicolás Daniel

    Publicado 2017
    “…Therefore, in addition to the traditional screening of natural nitrogen-fixing isolates, the genome engineering of model strains could be an attractive strategy to improve nitrogen fixation in legume crops.…”
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    info:eu-repo/semantics/article
  6. Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism por Bermudez Salazar, Luisa, de Godoy, Fabiana, Baldet, Pierre, Demarco, Diego, Osorio, Sonia, Quadrana, Leandro Daniel, Almeida de Souza, Juliana Beatriz, Asis, Ramón, Gibon, Yves, Fernie, Alisdair R., Rossi, Maria Magdalena, Carrari, Fernando

    Publicado 2019
    “…Limitations in our understanding about the mechanisms that underlie source-sink assimilate partitioning are increasingly becoming a major hurdle for crop yield enhancement via metabolic engineering. By means of a comprehensive approach, this work reports the functional characterization of a DnaJ chaperone related-protein (named as SPA; sugar partition-affecting) that is involved in assimilate partitioning in tomato plants. …”
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    info:ar-repo/semantics/artículo

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