Search Results - "electrophoresis"

Morphological and isozyme characterisation of Arachis pintoi Krap. et Greg. nom. nud. germplasm by Maass, Brigitte L., Torres González, Alba Marina, Ocampo Nahar, César H.

“…Polyacrylamide gel electrophoresis (PAGE) was applied to root-tip tissue. …”
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La enfermedad cuero de sapo en yuca (Manihot esculenta Crantz) by Pineda López, Benjamín, Jayasinghe, W. Upali, Lozano, J.C.

“…A technique to detect the disease by electrophoresis has been developed. In endemic regions cuttings from the most severely affected, vigorous plants (thickest stems) are selected. …”
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Conformationally Restricted Peptides from Rice Proteins Elicit Antibodies That Recognize the Corresponding Native Protein in ELISA Assays by Barrera, Nubia F., Melgarejo, Luz M., Cruz Gallego, Maribel, Cortés, Lina Jimena, Guzmán, Fanny, Calvo, Julio C.

“…By comparing proteomic profiles, between virus-infected and control group plants in two-dimensional electrophoresis, proteins exhibiting significant changes in abundance were found. …”
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Differentiation of citrus tristeza virus isolates by serological analysis of p25 coat protein peptide maps by Albiach-Martí, María R., Guerri, José, Cambra, Mariano, Garnsey, Stephen M., Moreno, Pedro

“…CTV particles were concentrated by centrifugation and purified by agarose gel electrophoresis. The CP was extracted from gel slices riched in virions and protein yields were about three times greater than those obtained previously and of comparable purity. …”
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Evaluation of cytotoxicity assays and a high-througput variant of the alkaline Comet assay in mouse lymphoma L5178Y cells by Gustavsson, Linda

“…The Comet assay, or Single Cell Gel Electrophoresis Assay, is a fast, easy and relatively inexpensive method to visualize and measure DNA strand breaks in individual cells. …”

Adición de proteínas del plasma seminal ovino durante la congelación del espermatozoide y efectos sobre su motilidad y viabilidad by Cardozo, Jaime Antonio, Grasa, Patricia, Muiño B., María Teresa, Cebrián P., José Álvaro

“…Comparative two-dimensional polyacrilamide gel electrophoresis analysis between fresh and frozen semen, either with or without seminal plasma proteins in the cryopreservation medium, revealed that eight protein spots were lost in frozen-thawed sperm. …”
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Påvisande av Helicobacter spp hos hund : en metodologisk studie by Nicol, Claire

“…The study shows that the Multiplex PCR works well, but that the capillary electrophoresis is not able to illustrate the results. …”

Characterisation of accessions held at the International Musa Genebank by Hribova, E., Cizkova, J., Houwe, Ines van den, Swennen, Rony L., Roux, N., Doležel, Jaroslav

“…This platform is based on 19 microsatellite markers which are scored using fluorescently labelled primers and high-throughput capillary electrophoresis separation with high resolution. In order to characterize selected ITC accessions in more detail, we analyzed their ITS sequences and studied phylogenetic relationships within the Musaceae family. …”
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Rolling circle amplification to screen yam germplasm for badnavirus infections and to amplify and characterise novel badnavirus genomes by Bömer, M., Turaki, A., Rathnayake, A., Silva, G., Kumar, P. Lava, Seal, S.

“…We proposed this to be a complementary method to denaturing gradient gel electrophoresis, which enables a rapid indication of badnavirus diversity as well as the identification of potentially integrated badnavirus sequences in the host genome (Turaki et al., 2017). …”
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Blood cellular changes associated with bacteremia and malaria co-morbidity among children in western Kenya by Ogulla, G., Mwalimu, Stephen, Muturi, M., Ouma, C.

“…The haemoglobin genotypes were analysed using the electrophoresis technique. Results: Children with haemoglobin AA and AS had elevated granulocyte counts. …”
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The major histocompatibility complex region and diversity of the local chicken populations in Niger by Hassan Ousseini, M., Machuka, Eunice M., Kyallo, Martina M., Tiambo, Christian K., Domelevo Entfellner, Jean-Baka, Pelle, Roger

“…Materials and methods: A total of 601 chickens from six local Nigerien chickens were sampled. By capillary electrophoresis using LEI0258 marker, 403 samples with different fragment sizes were randomly chosen and sequenced. …”
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The identification of circular extrachromosomal DNA in the nuclear genome of Trypanosoma brucei by Alsford, N.S., Navarro, M., Jamnadass, Ramni H., Dunbar, H., Ackroyd, M., Murphy, N.B., Gull, K., Ersfeld, K.

“…Restriction analysis of tagged NR-element DNA, unusual migration patterns during pulsed field gel electrophoresis (PFGE) and CsCl/ethidium bromide equilibrium centrifugation demonstrates that the NR-element represents circular DNA. …”
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PCR-DGGE analysis: unravelling complex mixtures of badnavirus sequences present in yam germplasm by Turaki, A.A., Bömer, M., Silva, G., Kumar, P. Lava, Seal, S.E.

“…In this study, the molecular diversity of partial reverse transcriptase (RT)-ribonuclease H (RNaseH) sequences from yam badnaviruses was analysed using PCR-dependent denaturing gradient gel electrophoresis (PCR-DGGE). This resulted in the identification of complex ‘fingerprints’ composed of multiple sequences of Dioscorea bacilliform viruses (DBVs). …”
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Proteomelevel change in the roots of Pisum sativum L. in response to salinity by Kav, N.N., Srivastava, S., Goonewardene, L., Blade, S.F.

“…Salinity-induced root proteome-level changes in pea were investigated by 2-D electrophoresis of proteins from control, 75 and 150 mM NaCl-treated plants and seedlings. …”
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A new virus on maize in Nigeria: maize mild mottle virus by Thottappilly, G., Qiu, W.P., Batten, J.S., Hughes, J.N., Scholthof, K.B.

“…The virions are composed of one single-stranded positive-sense RNA of approximately 4,000 nucleotides and, as estimated by 12.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), a capsid protein of approximately 28 kDa. …”
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Partial-Purification of a Virus-Associated with a Spanish Isolate of Citrus Ringspot by Navas-Castillo, J., Moreno, Pedro, Cambra, Mariano, Derrick, K.

“…RS-SR was partially purified by differential centrifugation, fractionation in a sucrose gradient, and agarose gel electrophoresis of selected fractions. Infectivity of concentrated extracts on C. quinoa was lost in individual fractions of the gradient, but it was recovered by combining a top and a bottom component. …”
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Lipopolysaccharides as Determinants of Serological Variability in Pseudomonas-Corrugata by Siverio, Felipe, Cambra, Mariano, Gorris, María T., Corzo, J., López, María M.

“…These results suggest that serology will not be a useful method for routine diagnosis of P. corrugata unless common antigens can be identified. Electrophoresis and immunoelectrotransfer were used to study the antigens involved. …”
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Changes in the Bark Proteins of Sour Orange Rootstock Induced by Citrus Tristeza Virus by Moreno, Pedro, Ortiz Marcide, Jesús M., Guerri, José

“…The effect of citrus tristeza virus (CTV) infection on bark proteins of several citrus rootstocks was studied by polyacrylamide gel electrophoresis (SDSPAGE). A protein band (P6) from bark of sour orange grafted with sweet orange, grapefruit or clementine scions had lower intensity in CTV-infected than in CTV-free trees. …”
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Genetic characterization of belly spot and splashed white coat color in horses by Eken, Helena

“…Genotypes were determined by PCR amplification of markers and capillary electrophoresis. Pedigree information and phenotypic information have been used to perform an association study and linkage analysis on the results. …”

Evaluation of ultrasound treatment on wine protein stability by Osorio B., Mónica S.

“…Further investigations should focus on identifying adequate bentonite concentrations to work in combination with ultrasound, determine ultrasound effect on protein concentration and conduct electrophoresis to identify wine protein structure.…”
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