Search Results - "electrophoresis"

Characterization of the seed proteins of velvet bean (Mucuna pruriens) from Nigeria by Machuka, J.

“…This paper reports the preliminary characterization of seed protein fractions from seven varieties of velvet beans (Mucuna pruriens) grown in Nigeria, using sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE). Three of the most abundant polypeptides, with approximate Mr of 23, 26 and 30 kDa, respectively, were further separated by preparative native-PAGE. …”
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Detection of Double-Stranded-Rna by Elisa and Dot Immunobinding Assay using an Antiserum to Synthetic Polynucleotides by Aramburu, Jose, Navas-Castillo, J., Moreno, Pedro, Cambra, Mariano

“…These techniques showed similar or higher sensitivity for detection of dsRNA than separation by polyacrylamide gel electrophoresis and silver staining.…”
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Microsatellite DNA fingerprinting of Coffea sp. germplasm conserved in Costa Rica through singleplex and multiplex PCR by Sánchez, Elodia, Solano, William, Gatica-Arias, Andrés, Chavarría, Max, Araya-Valverde, Emanuel

“…In this study, microsatellite DNA fingerprinting of coffee through singleplex and multiplex PCR approaches coupled with capillary electrophoresis are described. To validate both methods, germplasm of Coffea spp. …”
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Identification of proteins expressed by the root-rot fungus Heterobasidion annosum when growing on lignocellulose by Wang, Xiaoyu

“…Extracellular proteins from one culture with spruce as carbon source were detected in electrophoresis after separation by cation and anion exchange chromatography. …”

A reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting the pathogen of maize rough dwarf disease in China by Du, Linlin, Shi, Wenjuan, Li, Xuejuan, Lan, Ying, Sun, Feng, Fan, Yongjian, Zhou, Tong, Zhou, Yijun

“…RT-LAMP products were analyzed by electrophoresis in agarose gels or visual inspection of color change after staining with calcein. …”
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Sexual hybridization among azolla species by Cat, Do Van, Watanabe, Iwao, Zimmerman, W.J., Lumpkin, T.A., Baillonville, T. de Waha

“…Hybrids from A. microphylla (female) and A. filiculoides (male) were studied in detail. Electrophoresis of enzymes, shikimate dehydrogenase, phosphoglucomutase, and 6-phosphogluconate dehydrogenase, showed that enzyme loci of hybrids shared those of one or both parents. …”
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Development of Ssr Markers from Citrus Clementina (Rutaceae) Bac End Sequences and Interspecific Transferability in Citrus by Ollitrault, Frederique, Terol, Javier, Pina, José A., Navarro, Luis, Talón, Manuel, Ollitrault, Patrick

“…A preliminary diversity study in Citrus was conducted with 18 of them, in C. reticulata, C. maxima, C. medica, C. sinensis, C. aurantium, C. paradisi, C. lemon, C. aurantifolia, and some papedas (wild citrus), using a capillary electrophoresis fragment analyzer. Intra- and interspecific polymorphism was observed, and heterozygous markers were identified for the different genotypes to be used for genetic mapping. …”
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Genetic Diversity in Cultivated Common Bean: II. Marker-Based Analysis of Morphological and Agronomic Traits by Singh, Shree, Gutiérrez, J.A., Molina, A., Urrea, C.

“…In addition, these same 306 landraces were characterized by electrophoresis for phaseolin seed protein and nine allozymes. …”
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Isozyme analysis of a tropical forest tree, Pterocarpus macrocarpus Kurz by Liengsiri, C., Yeh, F.C.H., Boyle, T.J.B.

“…Genetic variation within and among 11 natural populations of Pterocarpus macrocarpus Kurz. from different forest habitats in Thailand was examined by starch gel electrophoresis of 18 loci coding for 11 enzymes. The species possesses a high level of isozyme variation. …”
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Genetically discrete populations of Trypanosoma congolense from livestock on the Kenyan coast by Knowles, G., Betschart, K., Kukla, B.A., Scott, J.R., Majiwa, Phelix A.O.

“…Twenty-seven stocks of Nannomonas trypanosomes isolated from livestock in 1982 on a ranch at Kilifi on the Kenyan coast were characterized by isoenzyme electrophoresis and by the abilities of the parasite's DNA to hybridize to two repetitive sequence DNA probes. …”
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Etiología del "amachamiento" del frijol común en Costa Rica by Morales, Francisco José, Araya Fernández, CM, Hernández, J.C., Arroyave, José A., Cuervo Ibáñez, Maritza, Velasco, Ana Cecilia, Castaño J., Mauricio

“…The isometric virus was characterized by electron microscopy, serology, electrophoresis and nucleic acid analyses, as a strain, adapted to bean, of the cowpea clorotic mottle bromovirus (CCMV). …”
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Plant viroids: isolation, characterization/detection, and analysis. by Owens, Robert A., Sano, Teruo, Durán-Vila, Núria

“…Polyacrylamide gel electrophoresis (PAGE) was the first molecular technique to be used for the rapid (2-3 days) identification of viroid-infected plants. …”
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Growth and Morphogenesis of Citrus Tissue-Cultures Infected with Psorosis, Vein Enation, and Cachexia by Durán-Vila, Núria, Medina, Vicente, Pina, José A., Ortega, Carmen, Molins, M., Navarro, Luis

“…The citrus cachexia viroid was detected from infected callus by nucleic acid extraction and sequential polyacrylamide gel electrophoresis. Electron microscopy studies revealed alterations at the cell level on psorosis-infected callus.…”
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Exclusion and/or Uneven Distribution of Viroids in Four Citrus Hosts by Durán-Vila, Núria, Pina, José A., Navarro, Luis

“…The inoculated plants were biologically indexed on Etrog citron indicators, and were also analyzed by sequential polyacrylamide gel electrophoresis (sPAGE) of nucleic acid extracts. Citrons inoculated with infected Verna lemon and Nules clementine showed the severe reaction characteristic of the original isolate whereas Frost satsuma and Navelina sweet orange induced a milder reaction. …”
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Proteomics studies of Alzheimer’s Aβ-oligomers to identify interactions with other proteins in human serum and cerebrospinal fluid. by Rahman, Mahafuzur

“…The captured proteins have then been separated by gel electrophoresis and characterized by mass spectrometry. …”

Differential protein sysnthesis during the life cycle of the protozoan parasite Trypanosoma brucei by Shapiro, S.Z., Kimmel, A.E.

“…Two‐dimensional polyacrylamide gel electrophoresis has been used to analyze changes in protein content and protein synthesis in three stages of the life cycle of the protozoan parasite Trypanosoma brucei. …”
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Characterization of the in vitro core surface proteome of Mycoplasma mycoides subsp. mycoides, the causative agent of contagious bovine pleuropneumonia by Krasteva, I., Liljander, Anne M., Fischer, A., Smith, D.G.E., Inglis, N.F., Scacchia, M., Pini, A., Jores, Joerg, Sacchini, F.

“…Polyacrylamide gel electrophoresis and mass spectrometry were used to characterize the Triton X-114 soluble proteome of nine Mmm strains isolated from Europe or Africa. …”
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Host preference of tsetse: An important tool to appraise the Nagana risk of cattle in the cotton zone of Mali by Hoppenheit, A., Steuber, S., Bauer, B., Ouma, E.M., Diall, O., Zessin, K.H., Clausen, Peter-Henning

“…The subsequent use of cattle-specific primers yielded 52 visible amplicons in the gel electrophoresis. Sequencing and BLASTN® analysis of the remaining samples revealed 19 blood meals matching with existing sequences of the human genome in Genbank®. …”
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Genetics of esterase and glutamate oxaloacetate transaminase isozymes in cassava (Manihot esculenta Crantz) by Sarria, Rodrigo A., Ocampo Nahar, César H., Ramírez, H., Hershey, Clair H., Roca, W.

“…Genetic studies of two isozyme loci have been carried out in root tips of eight cassava F1 progenies using polyacrylamide gel electrophoresis. Two loci, esterase-1 (East-1), the most distant cationic region, and glutamate oxaloacetate transaminase-1 (Got-1), the most proximal anionic region, were evaluated among the segregating populations. …”
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Trypanosoma infection rates in Glossina species in Mtito Andei Division, Makueni County, Kenya by Nthiwa, D.M., Odongo, David O., Ochanda, H., Khamadi, S., Gichimu, B.M.

“…The DNA was then subjected to a nested PCR assay using internal transcribed spacer primers and individual trypanosome species were identified following agarose gel electrophoresis. Out of the 117 flies trapped in the area 39 (33.3%) were teneral while 78 (67%) were nonteneral. …”
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