Resultados de búsqueda - "electrophoresis"

A polymorphism in the bovine gamma-S-crystallin gene revealed by allele-specific amplification por Kemp, Stephen J., Maillard, J.C., Teale, A.J.

“…The two allele‐specific primers differ in length by 20 nucleotides so that the allelic products may be distinguished by simple agarose gel electrophoresis following a single PCR reaction. This provides a simple and rapid assay for this polymorphism.…”
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Genetic diversity of 10 indigenous chicken ecotypes from Southern Highlands of Tanzania based on Major Histocompatibility Complex-linked microsatellite LEI0258 marker typing por Mwambene, Pius L., Kyallo, Martina M., Machuka, Eunice M., Githae, Dedan, Pelle, Roger

“…A total of 400 DNA samples, 40 per ecotype, were genotyped by capillary electrophoresis. Thirty different alleles with sizes ranging from 197 to 569 bp were determined. …”
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Isozyme analysis and its application in plant breeding por Asiedu, Robert

“…Plant breeders face the difficult task of having to select for traits that are often under complex genetic control and subject to edapho-climatic influences. Electrophoresis of protein extracts from plant tissue, using different kinds ofsupport media and buffer systems, allows separation of the multiple forms ofenzymes (isozymes) on the basis of charge and/or molecular size. …”
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Restriction mapping of the genome of the protozoan parasite Theileria parva por Morzaria, S.P., Young, J.R.

“…We have used a modified linking clone strategy and pulsed-field gel electrophoresis to derive a map of the 29 Sfi I and 4 Not I sites in the 10 million base pair genome of the protozoan parasite Theileria parva. …”
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Biogeographical and molecular observations on Phaseolus glabellus (Fabaceae, Phaseolinae) and its taxonomic status por Schmit, Véronique, Debouck, Daniel G., Baudoin, Jean-Pierre

“…Biogeographic considerations as well as experimental hybridization, seed storage protein electrophoresis and cpDNA polymorphism point at its isolated taxonomic position and distinctness from P. coccineus. …”
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Golgi-associated phosphohydrolases in Trypanosoma brucei brucei por Grab, D.J., Webster, P., Verjee, Y., Lonsdale-Eccles, J.

“…Using the powerful technique preparative free-flow electrophoresis [29] which has been used to T. brucei [13] it is showed that Golgi-associated tartrate-sensitive acid phosphatase is located in a sub-component of the Golgi fraction of T. brucei lysates. …”
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Electroejaculation Increases Low Molecular Weight Proteins in Seminal Plasma Modifying Sperm Quality in Corriedale Rams por Ledesma, Alba, Manes, Jorgelina, Cesari, Andreina, Alberio, Ricardo, Hozbor, Federico Andres

“…Volume, pH, osmolarity, protein concentration, total protein content and protein profile using sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS‐PAGE) and 2‐D polyacrylamide electrophoresis of seminal plasma collected with artificial vagina and electroejaculation were also analysed. …”
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Metoder för påvisande av MRSA hos häst por Corin, Antonia

“…Pulsed-field gel electrophoresis (PFGE) har i flera år varit den mest använda metoden vid typning av isolat men har mer och mer börjat ersättas av bland annat spa-typning och multi-locus sequence typing (MLST). …”

Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone por Wambugu, J., Kanguha, E., Hanotte, Olivier H., Davis, S., Taylor, J., Skow, L., Brunner, R., Schwerin, M., Teale, A.J., Iraqi, F.A.

“…The PCR product length was polymorphic as shown by denaturing polyacrylamide gel electrophoresis. The microsatellites were designated as ILFI-TNF-I and II for (GT)9 and (TA)10(TTA)5, respectively.…”
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Genetics of two colonies of Glossina pallidipes originating from allopatric populations in Kenya por Gooding, R.H., Moloo, S.K.

“…Abstract Two large colonies, originating from allopatric populations of Glossina pallidipes Austen, in the Shimba Hills and Nguruman, Kenya, which differ biologically and with respect to vectorial competence, were compared at fourteen enzyme loci using polyacrylamide gel electrophoresis. The colonies had similar levels of genetic diversity with approximately half of the loci being polymorphic, an average of 1.6‐1.7 alleles per locus, and a mean heterozygosity per locus of approximately 18.4%. …”
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Application of septenary multiplex PCR detection for some animal components in sheep and goat meat products por Feng, H., Han, J.

“…The results showed that routine agarose gel electrophoresis was sufficient to differentiate the multiplex PCR products ranging from 175 to 517 bp and differing by at least 41 bp each other, indicating that this septenary multiplex PCR method could be used for rapid and accurate identification of the seven species and for three species (cow, yak and goat) at sensitivity of 2.5 ng DNA. …”
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Polymorphisme de la transferrine chez les bovins trypanosensibles et trypanotolerants de l'Afrique de l'Ouest. Repartition et frequence de leurs alleles por Queval, R.

“…E.C.2.4.2.1) controlled by the alleles NP.H and NP.L was determined on erythrocyte lysates from zebu, Baoule and N'Dama/Baoule cross cattle. Electrophoresis was performed on cellulose acetate and NP revealed. …”
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Comparative Levels of Waxy Gene Product of Endosperm Starch Granules of Different Rice Ecotypes por Villareal, C.P., Juliano, Bienvenido O.

“…Starches of 40 rice varieties from different ecosystems and 2 amylose extender mutants were analyzed for their predominant waxy gene allele (Wxa or Wxb) through the amount of Wx gene product (60‐kdal polypeptide) by SDS‐polyacrylamide gel electrophoresis. Nonwaxy rices carrying the Wxa and Wxb alleles were clearly differentiated by two distinct regression equations correlating AC and the amount of Wx gene product. …”
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A comparison of Glossina morsitans centralis originating from Tanzania and Zambia, with respect to vectorial competence for pathogenic Trypanosoma species, genetic variation and in... por Moloo, S.K., Gooding, R.H.

“…Nevertheless, these 2 laboratory strains of G. m. centralis showed levels of susceptibility to the 3 pathogenic Trypanosoma species which were much greater than previously observed in laboratory colonies of other Glossina species. Electrophoresis of 15 enzymes revealed that the 2 colonies differ significantly in allele frequencies at only 3 loci that are relatively close together on one of the autosomes. …”
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Viroids as Companions of a Professional Career por Durán-Vila, Núria

“…Sucrose density gradient centrifugation and polyacrylamide gel electrophoresis (PAGE) played a critical role in the pioneering studies of PSTVd and citrus exocortis viroid (CEVd). …”
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Proteolysis in UHT milk due to Staphylococcus aureus por Crnadak, Ninoslava

“…Samples were stored at -200C until analysis for protein separation by capillary electrophoresis (CE). CE was run for two strains (333 and 485) and electropherograms were obtained. …”

Antimicrobial and insecticidal protein isolated from seeds of Clitoria ternatea (L.), a tropical forage legume por Kelemu, Segenet, Cardona, C., Segura, G.

“…A single protein band was obtained on both sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and IEF gels. The protein, designated ‘finotin’, has broad and potent inhibitory effect on the growth of various important fungal pathogens of plants, namely Rhizoctonia solani, Fusarium solani, Colletotrichum lindemuthianum, Lasiodiplodia theobromae, Pyricularia grisea, Bipolaris oryzae and Colletotrichum gloeosporioides. …”
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Genetic variation in Glossina brevipalpis, Glossina longipennis and Glossina pallidipes and the phenetic relationships of Glossina species por Gooding, R.H., Moloo, S.K., Rolseth, B.M.

“…Glossina brevipalpis Newstead, G.longipennis Corti, and G.pallidipes Austen maintained at ILRAD, Nairobi, Kenya, were examined for genetic variation of fourteen enzyme loci, using poly‐acrylamide gel electrophoresis. G.brevipalpis had six polymorphic loci, an average of 1.46 effective alleles per locus and a mean heterozygosity per locus of 20.0±7.1%. …”
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Distinction of African trypanosome species using nucleic acid hybridization por Massamba, N.N., Williams, R.O.

“…We have performed restriction endonuclease digestion of nuclear DNAs, combined with gel electrophoresis and molecular hybridization to characterize different Trypanosoma brucei brucei stocks and to identify trypanosome species. …”
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One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins por Machuka, J., Okeola, O.

“…One- and two-dimensional polyacrylamide gel electrophoresis was then used to analyze the albumin fraction, galactose-specific lectins purified on immobilized galactose−Sepharose 4B, and abundant non-lectin seed proteins left over following affinity chromatography. …”
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