Search Results - "electrophoresis"

Analysis of MHC class II and monogenic eye diseases in the Scandinavian wolf population by Marby, Davida

“…The analysis of eye diseases was performed with either real time PCR (TaqMan) or capillary electrophoresis (ABIPrism 3100). To gain further understanding about the ge-netic complexity and degree of genetic variation in the Scandinavian wolf population, the wolves were also sampled and sequenced for their genetic variation in the MHC class II loci (DLA-DRB1, DLA,DQA1 and DLA-DQB1). …”

First isolation of Salmonella enterica serovar Soerenga from pregnant sows in Argentina = Primer aislamiento de Salmonella enterica serovar Soerenga a partir de cerdas gestantes... by Joaquim, Patricia Estefania, Herrera, Mariana, Moroni, Mirian, Chacana, Pablo

“…Moreover, resistance to 17 antimicrobials and presence of 10 virulence genes and pulsed field gel electrophoresis analysis was performed to characterize the isolate, which was resistant to erythromycin, tiamulin and tylosin, and carried the virulence genes sopB, ssaQ, mgtC, avrA and bcfC. …”
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Peanut seed cultivars with contrasting resistance to Aspergillus parasiticus colonization display differential temporal response of protease inhibitors by Muller, Virginia, Bonacci, Gustavo Roberto, Batthyany, Carlos, Amé, María Valeria, Carrari, Fernando, Gieco, Jorge Omar, Asis, Ramón

“…With chromatographic methods and 2D-electrophoresis-mass spectrometry we have isolated and identified four variants of Bowman-Birk trypsin inhibitor (BBTI) and a novel Kunitz-type protease inhibitor (KPI) produced in response to A. parasiticus colonization. …”
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Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage by Perotti, Valeria Elisa, del Vecchio, Hernán A., Sansevich, Alina, Meier, Guillermo Enrique, Bello, Fernando, Cocco, Mariángeles, Garran, Sergio Mario, Anderson, Catalina Margarita, Vazquez, Daniel Eduardo, Podesta, Florencio Esteban

“…Differentially expressed proteins were detected by two-dimensional differential gel electrophoresis and identified by matrix-assisted laser desorption/ionization MS. …”
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Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure by Perotti, Valeria Elisa, Moreno, Alejandra Soledad, Tripodi, Karina Eva Josefina, Meier, Guillermo Enrique, Bello, Fernando, Cocco, Mariángeles, Vazquez, Daniel Eduardo, Anderson, Catalina Margarita, Podesta, Florencio Esteban

“…Using two‐dimensional differential gel electrophoresis to analyze exposed and non‐exposed fruit, 27 differential protein spots were detected in juice sacs and flavedo, among all comparisons made. …”
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Genetic and biochemical biomarkers in the macrophyte Bidens laevis L. Exposed to a commercial formulation of endosulfan by Pérez, Débora Jesabel, Lukaszewicz, Germán, Menone, Mirta Lujan, Amé, María Valeria, Camadro, Elsa Lucila

“…Previous studies in the wetland macrophyte Bidens laevis L have demonstrated that the insecticide endosulfan induces a high frequency of somatic chromosome aberrations in anaphase–telophase (CAAT) but no DNA changes as determined by the single cell gel electrophoresis (Comet) assay. Thus, cytogenetic biomarkers appear to be more sensitive to the toxic effects of the insecticide than the DNA molecule in the studied species. …”
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Comparación de metodologías para la purificación de proteínas de membrana externa de Brucella abortus by Forero de Lleras, Claudia, Rueda de Clavijo, Esperanza, Deleón, Ligia S. de, Mariño, Olga C., Montes de Gómez, Virginia

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Listeria monocytogenes i vakuumförpackad lax by Mandorf, Elisabeth

“…Isolates from the positive samples were cleaved with restriction enzymes (REA) and analysed with Pulsed-Field Gel Electrophoresis (PFGE). All of the clonal types found in the samples have previously been isolated from humans suffering from listeriosis. …”

Bio-ecology and systematics of the scale insect Stictococcus vayssierei Richard, 1971 (Hemiptera: Stictococcidae), a major pest of root and tuber crops in the Nigero-Congolian fore... by Doumtsop, F.A.R.

“…Denaturing gradient gel electrophoresis and diagnostic PCR performed on the 16S rRNA bacterial gene using total DNA indicated that ARTS’populations were infected with a primary endosymbiont close to Uzinura diaspidicola of armored scale insects, three secondary endosymbionts including Rickettsia, Serratia and Wolbachia, and an uncultured gamma-proteobacterium. …”
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First report of Xanthomonas oryzae pv. oryzicola causing bacterial leaf streak of rice in Burundi by Afolabi, O., Milan, B., Amoussa, R., Koebnik, R., Poulin, L., Szurek, B., Habarugira, G., Bigirimana, J., Silué, D.

“…Three DNA fragments (331, 691, and 945 bp) corresponding to X. oryzae pv. oryzicola were observed after agarose gel electrophoresis. Single bacterial colonies were then isolated from surface-sterilized, infected leaves after grinding in sterile water and plating of 10-fold dilutions of the cell suspension on semi-selective PSA medium (4). …”
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Nitrogen–sulfur fertilisation effects on gluten composition and industrial quality in Argentinean bread wheat cultivars differing in apparent sulfur recovery by Arata, Agustin Francisco, Rogers, Willian, Tranquilli, Gabriela, Arrigoni, Adriana C., Rondanini, Deborah Paola

“…Gluten composition was analysed by electrophoresis and densitometry, and grain quality by N/S ratio, protein content, sedimentation test, and alveograms. …”
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Association of an IRF3 putative functional uORF variant with resistance to Brucella infection: A candidate gene based analysis of InDel polymorphisms in goats by Rossi, Ursula, Hasenauer, Flavia Carolina, Caffaro, María Eugenia, Raschia, Maria Agustina, Maurizio, Estefania, Cortez, Hector Sergio, Neumann, Roberto Daniel, Poli, Mario Andres, Rossetti, Carlos Alberto

“…InDels (IRF3-540: rs660531540, FKBP5-294: rs448529294, TIRAP-561: rs657494561, PTPRT-588: rs667380588, KALRN-989: rs667660989 and RAB5a-016: rs661537016) were resolved by PCR-capillary electrophoresis in samples from 64 cases and 64 controls for brucellosis. …”
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Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel by Capella, Virginia, Rivero, Rebeca E., Liaudat, Ana Cecilia, Ibarra, Luis Exequiel, Roma, Dardo Andrés, Alustiza, Fabrisio Eduardo, Mañas, Fernando, Barbero, César A., Bosch, Pablo, Rivarola, Claudia R., Rodríguez, Nancy

“…Genotoxicity was evaluated by the single-cell gel electrophoresis assay, where DNA damages were not detected. [3H]-thymidine staining allowed to corroborate that cell proliferation had progressed correctly. …”
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Genotypic Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Beef Abattoirs of Argentina by Masana, Marcelo Oscar, D’Astek, Beatriz A., Palladino, Pablo Martin, Galli, Lucia, Del Castillo, Lourdes, Carbonari, Claudia Carolina, Leotta, Gerardo A., Vilacoba, E., Irino, K., Rivas, Marta

“…XbaI pulsed-field gel electrophoresis pattern analysis showed 234 different patterns, with 76 strains grouped in 30 clusters. …”
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Ingen påvisad förekomst av cetacean morbillivirus hos svenska tumlare by Axelsson, Elin

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Genotyping ethylene production genes Md-ACS1 and Md-ACO1 for markerassisted selection in apple by Lundmark, Johan

“…In this study, capillary electrophoresis was used to screen 255 cultivars from Nordic and Baltic germplasm collections for alleles at the Md-ACS1 and Md-ACO1 loci. …”
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A colorimetric, sensitive, rapid, and simple diagnostic kit for the HLB putative causal agent detection by Stolowicz, Fabiana, Larocca, Luciana, Werbajh, Santiago, Parma, Yanil, Carrillo, Carolina, Ogas, Lorena, Agostini, Juan Pedro, Redes, Jonathan, Welin, Bjorn, Castagnaro, Atilio, Vojnov, Adrian

“…Similar sensitivity to visualize a positive reaction was observed between HNB loop-mediated isothermal amplification and agarose gel electrophoresis analysis. The detection of C.Las-infected plants was up to 8 ng of total infected plant genomic DNA, similar to quantitative PCR. …”
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Development of a ptp2-LAMP assay for the specific and sensitive detection of Nosema apis and its comparison with ptp3-LAMP for the detection of Nosema ceranae, in a region endemi... by Lannutti, Lucas, Gisder, Sebastian, Florin-Christensen, Monica, Genersch, Elke, Schnittger, Leonhard

“…Amplification was verified by agarose gel electrophoresis (conventional format), and by a change from pink to yellow color after addition of a suitable dye (colorimetric format). …”
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In vitro and in vivo screening of native lactic acid bacteria toward their selection as a probiotic in broiler chickens by Blajman, Jesica E., Gaziano, Cristian, Zbrun, María Virginia, Soto, Lorena Paola, Astesana, Diego Martín, Berisvil, Ayelén Patricia, Romero Scharpen, Analía, Signorini, Marcelo, Frizzo, Laureano Sebastian

“…L. salivarius DSPV 001P administered to broilers and recovered from GIT was compared by pulsed-field gel electrophoresis (PFGE) to ensure that the same genotype was persistently identified. …”
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Combined epidermal growth factor and hyaluronic acid supplementation of in vitro maturation medium and its impact on bovine oocyte proteome and competence by Rios, Glenda Laura, Buschiazzo, Jorgelina, Mucci, Nicolas Crescencio, Kaiser, German Gustavo, Cesari, Andreina, Alberio, Ricardo

“…In addition, the impact of IVM conditions on the proteomic profile of metaphase II bovine oocytes was analyzed by two-dimensional electrophoresis. Cumulus–oocyte complexes were matured in two media: (1) 10 ng/mL EGF, 15 μg/mL HA, and 100-μM cysteamine and (2) 0.01 UI/mL rh-FSH and 10% FBS. …”
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