Search Results - "electrophoresis"

Genetic variability within accessions of the B73 maize inbred line by Olmos, Sofí­a Eugenia, Lorea, Roberto Daniel, Eyherabide, Guillermo

“…The genotyping technique used in this work allowed the separation of alleles of ± 2 bp range difference within the same electrophoresis run, whereas allele size estimations between experiments, within the laboratory, differed in ± 4 bp range difference. …”
Get full text
Get full text

Aggregation kinetics of humic acids in the presence of calcium ions by Kloster, Nanci Soledad, Brigante, Maximiliano Eduardo, Zanini, Graciela Pilar, Avena, Marcelo Javier

“…UV–VIS spectroscopy was employed to quantify the progress of the aggregation reaction, and electrophoresis was used to evaluate the zeta potential of the HA molecules. …”
Get full text
Get full text
Get full text

Embryo Culture of Lycopersicon esculentum × L. peruvianum Hybrid Genotypes Possessing Heat-stable Resistance to Meloidogyne incognita by Cap, Guillermo Bartolome, Roberts, P.A., Thomason, I.J., Murashige, T.

“…Hybridity of the F, progeny obtained from each cross was confirmed by differences in leaf and flower morphology, plant growth habits, and by acid phosphatase isozyme phenotypes using polyacrylamide gel electrophoresis. In greenhouse inoculation experiments, F1 plants were highly resistant to M. incognita in soil at 25 and 30C. …”
Get full text
Get full text
Get full text

Identification and Preliminary Characterization of a Viroid-like RNA in Atalantia citroides by Barbosa, C. J., Serra, Pedro, Pina, José A., Navarro, Luis, Daros, José A., Flores, Ricardo, Durán-Vila, Núria

“…However, sequential polyacrylamide gel electrophoresis (sPAGE) showed the presence in the A. citroides scion of a viroid-like RNA with an electrophoretic mobility between HSVd and CVd-III. …”
Get full text
Get full text

Tollarsjuka hos hund : en retrospektiv studie med journalsammanställning av 18 hundar by Veien, Kristi Anne

“…Hematological and serological examination of the blood revealed no changes of importance for the development of "tollarsjuka". Serum protein electrophoresis was performed in 12 dogs and documented a mild decrease in the concentration of albumin. …”

Characterization of bovine milk from indigenous species from Sri Lanka by Skager, Jonathan

“…A total of 15 milk samples from each cattle type were analyzed by a fluorescence method for total proteolysis and by capillary electrophoresis for identification and quantification of the protein profiles. …”
Get full text

Biopreservation effects with Wickerhamomyces anomalus after 2 months moist airtight storage of sorghum by Nybogård, Louise

“…Quantification and identification of Enterobacteriaceae, yeast, moulds and lactic acid bacteria (LAB) were performed by serial dilution on microbiological media, PCR, electrophoresis, DNA sequencing and database comparison. …”
Get full text

Identification of candidate vaccine antigens of bovine hemoparasites Theileria parva and Babesia bovis by use of helper T cell clones by Brown, W.C., Zhao, S., Logan, K.S., Grab, D.J., Rice-Ficht, A.C.

“…Proteins separated by gelfiltration revealed dour patterns of reactivity, and proteins separated by anion exchange revealed two patterns of reactivity when selected T cell clones were assayed for stimulation by antigenic fractions.Studies using a continuous-flow electrophoresis apparatus have indicated the feasibility of identifying T cell-stimulatory proteins from parasite membranes as well as from the cytosolic fraction of B. bovis merozoites. …”
Get full text

Lysosomal and non-lysosomal peptidyl hydrolases of the bloodstream forms of Trypanosoma brucei brucei by Lonsdale-Eccles, John D., Grab, D.J.

“…The cathepsin‐L‐like activity is inhibited by the thiol‐protease inhibitors, E‐64, cystatin, leupeptin and mercurial compounds.The proteolytic activity of the lysosome‐like fraction is observed as a single band of activity with an approximate molecular mass of 27 kDa when measured after electrophoresis in the fibrinogen‐containing sodium dodecyl sulphate/polyacrylamide gels. …”
Get full text

Development and validation of allele-specific SNP/indel markers for eight yield-enhancing genes using whole-genome sequencing strategy to increase yield potential of rice, Oryza sa... by Kim, Sung-Ryul, Ramos, Joie, Ashikari, Motoyuki, Virk, Parminder S., Torres, Edgar A., Nissila, Eero, Hechanova, Sherry Lou, Mauleon, Ramil, Jena, Kshirod K.

“…Automated capillary electrophoresis was tested and fluorescence-labeled SNP genotyping markers (Fluidigm SNP genotyping platform) for Gn1a, OsSPL14, Ghd7, GS5, and GS3 genes were developed for high-throughput genotyping. …”
Get full text

Rapid plant DNA and RNA extraction protocol using a bench drill by Ferreira, C.F, Gutiérrez Reynoso, Dina Lida, Kreuze, Jan, Iskra Caruana, M.L, Chabannes, M, Barbosa, A.C.O, Santos, T.A, Silva, A.G.S, Amorim, E.P, De Oliveira, S.A.S, Jesus, O.N

“…Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. …”
Get full text
Get full text

Determination of alkaloids in onion nectar by micellar electrokinetic chromatography by Soto Vargas, Verónica Carolina, Jofre, Viviana Patricia, Galmarini, Claudio Romulo, Silva, Maria Fernanda

Get full text
Get full text

Comparative study of the protein profiles of Sunki mandarin and Rangpur lime plants in response to water deficit by Oliveira, Tahise M., da Silva, Fernanda R., Bonatto, Diego, Neves, Diana M., Morillon, Raphael, Maserti, Bianca E., Coelho Filho, Mauricio A., Costa, Marcio G. C., Pirovani, Carlos P., Gesteira, Abelmon S.

“…We performed two-dimensional electrophoresis (2-DE) separation followed by LC/MS/MS to assess the proteome responses of the roots of two citrus rootstocks, Rangpur lime (Citrus limonia Osbeck) and ‘Sunki Maravilha’ (Citrus sunki) mandarin, which show contrasting tolerances to water deficits at the physiological and molecular levels. …”
Get full text

Analysis of the methanogenic and acetogenic community structure in young calves by Lenngren Hysing, Paulina

“…Fecal samples from one 6 months old and one 2 weeks old calf were chosen for generation of a library for the methanogens whereas for the acetogens, four libraries were created from a fecal sample from a 6 month old calf and feces, rumen- and cecum digesta from a 2 days old calf. Gel electrophoresis of the PCR confirmed presence of methanogens in 21 faecal samples from calves 14 days of age and older. …”

Analys av KC-like som biomarkör för sepsis hos tikar med pyometra by Klemming, Caroline

“…De utvalda CXCL-kemokinerna uttrycktes därför i Escherichia coli och analyserades med Sodium Dodecyl Sulfate-Polyacrylamid Gel Electrophoresis (SDS-PAGE) och western blot. Blodprover insamlade från tikar med pyometra med eller utan SIRS analyserades också med en Enzyme-linked Immunosorbent Assay (ELISA) med KC-antikropp hos mus. …”

Immunization studies in Rabbits using midgut membrane bound protein derived from rhipicephalus appendiculatus, rhipicephalus evertsi evertsi and amblyomma variegatum. by Kutima, H.L.

“…Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of the Gut membrane-Bound Protein (GMBP) antigens demonstrated protein bands with molecular weights ranging from 14 to 140 kDa. …”
Get full text

Purification, physicochemical and funtional characterisation of Glossina Morsitans centralis fibrinolysins. by Endege, W.O.

“…They appeared electrophoretica1ly homogeneous as judged by SDS polyacrylamide electrophoresis (PAGE). The two enzymes seem to digest fibrinogen and fibrin at different sites but within the same domains as does bovine plasmin, a classical fibrinolysin. …”
Get full text

Genetic diversity in a world germplasm collection of tall fescue by Cuyeu, Alba Romina, Rosso, Beatriz Susana, Pagano, Elba Maria, Soto, Gabriela, Fox, Ana Romina, Ayub, Nicolás Daniel

“…In this study, we explored the genetic diversity of 161 accessions of Festuca species from 29 countries, including 28 accessions of INTA (Argentina), by analyzing 15 polymorphic SSR markers by capillary electrophoresis. These molecular markers allowed us to detect a total of 214 alleles. …”
Get full text
Get full text

Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia by Ágredo-Campos, Ángela Sofía, Camussone, Cecilia, Akineden, Ömer, Fernández-Silva, Jorge A., Ramírez-Vásquez, Nicolás F.

“…The clonal relationship between the isolates was assessed using macrorestriction fragment analysis (MRFA) of chromosomal DNA by pulsed-field gel electrophoresis (PFGE). Most of the isolates showed susceptibility to the tested antibiotics; 19% exhibited resistance to tetracycline and all isolates showed beta-lactamases. …”
Get full text
Get full text
Get full text

Genetic analysis of the 3′ untranslated region of the bovine SLC11A1 gene reveals novel polymorphisms by Hasenauer, Flavia Carolina, Caffaro, María Eugenia, Czibener, Cecilia, Comerci, Diego José, Poli, Mario Andres, Rossetti, Carlos Alberto

“…The genotyping by capillary electrophoresis showed the presence of four alleles (157, 159, 161 and 163) for the first microsatellite (MS1) and six alleles (175, 177, 179, 181, 183 and 185) for the second microsatellite (MS2). …”
Get full text
Get full text
Get full text