Resultados de búsqueda - "electrophoresis"

Studies on genetic polymorphism of improved cowpea varieties using Simple Sequence Repeats (SSR) marker por Olasan, O.J., Omoigui, L., Oluma, H.A., Aguoru, C.U., Deo, D., Ugbaa, M.S., Ezugwu, J., Ekeruo, G., Dughduh, P., Iyorkaa, N., Simon, M., Okoh, T.

“…A total of 10 SSR primers were used in the DNA amplification process on a programmed thermal cycler followed by electrophoresis, visualization and scoring of the banding pattern. …”
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Reverse Transcriptase Loop-Mediated Isothermal Amplification Assays (RTLAMP) for the Detection of Alfalfa Mosaic Virus from Forage Crops por Dawit, Woubit, Mulatu, Fikerte, Eshete, Yesuf, Negawo, Alemayehu T., Muchugi, Alice, Jones, Christopher S.

“…Amplicons were easily visualized by means of an in-tube colour indicator (SYBR Green I dye) with no requirement to run gel electrophoresis. The sensitivity of the RT-LAMP assay was assessed by comparing the optimized AMV RT-LAMP assay with the conventional RT-PCR. …”
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Disease development and enhanced susceptibility of wounded pepper roots to phytophthora capsici por Adorada, D.L., Biles, C.L., Liddell, C.M., Fernández-Pavía, S., Waugh, K.O., Waugh, M.E.

“…Isoelectric focusing‐polyacrylamide gel electrophoresis (IEF‐PAGE) indicated increased band intensity of three acidic and one basic isozyme as wounds aged. …”
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Trypanosomatid cysteine protease activity may be enhanced by a kininogen-like moiety from host serum por Lonsdale-Eccles, John D., Mpimbaza, G.W.N., Nkhungulu, Z.R.M., Olobo, J., Grab, D.J.

“…However, two additional bands of trypanopain activity (87 kDa and 105 kDa) are observed if serum is added to the trypanopain before electrophoresis. Formation of the 87 and 105 kDa bands is frequently accompained by a reduction in the intensity of the 28 kDa activity which suggests that the extra bands are complexes of the 28 kDa trypanopain-Tb and a molecule from rat serum called rat trypanopain moledulator (rTM). …”
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Comparisons of protein profiles of beech bark disease resistant and susceptible American beech (Fagus grandifolia) por Mason, M.E., Koch, J.L., Krasowski, M., Loo, J.

“…Sixteen trees were sampled from eight geographically isolated stands, the sample consisting of 10 healthy (scale-resistant) and 6 diseased/infested (scale-susceptible) trees.Proteins were extracted from each tree and analysed in triplicate by isoelectric focusing followed by denaturing gel electrophoresis. Gels were stained and protein spots identified and intensity quantified, then a statistical model was fit to identify significant differences between trees. …”
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Analysis of the immunoproteome of Mycoplasma mycoides subsp. mycoides small colony type reveals immunogenic homologues to other known virulence traits in related Mycoplasma species por Jores, Joerg, Meens, J., Buettner, F.F.R., Linz, B., Naessens, Jan, Gerlach, G.F.

“…In this study we used two-dimensional polyacrylamide gel electrophoresis followed by immunoblot with sera from MmmSC-infected animals and MALDI-ToF mass spectrometry to identify novel immunogenic proteins as candidate molecules for improved diagnostics and vaccines. …”
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Induction of a major leaf acid phosphatase does not confer adaptation to low phosphorus availability in common bean por Yan, X, Liao, H, Trull, MC, Beebe, Stephen E., Lynch, J

“…Non-denaturing protein electrophoresis revealed that there are four visible isoforms responsible for total APA in common bean, and that the difference in APA between contrasting genotypes could be attributed to the existence of a single major isoform. …”
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Isolation and Characterization of Intestinal Escherichia coli Clones from Wild Boars in Germany por Schierack, P., Römer, A., Jores, Joerg, Kaspar, H., Guenther, S., Filter, M., Eichberg, J., Wieler, L.H.

“…Ten isolates per section were subjected to clonal determination using pulsed-field gel electrophoresis. One representative isolate per clone was further investigated for virulence traits, phylogenetic affiliation, and antimicrobial susceptibility. …”
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Evaluation of response to treatment of mixed trypanosome infection in goats using the polymerase chain reaction por Burudi, E.M.E., Peregrine, A.S., Majiwa, Phelix A.O., Murphy, N.B.

“…The sensitivity of the PCR technique for detecting Il 1180 genomic DNA, when mixed with 25 ng total genomic DNA of IL 3274 was 100 pg by gel electrophoresis and ethidium bromide staining of the PCR products. …”
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Characterization of some Goat Populations in Ethiopia by means of Blood Protein Polymorphism por Simachew, Addis

“…The red cell lysates and plasma proteins were analyzed by starch gel electrophoresis. From a total of five loci studied, two were polymorphic in, at least one population. …”
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Isolation and characterisation of a gene encoding a potential vaccine and diagnostic antigen of theileria lestoquardi. por Mjombah, N.S.S.

“…Following analysis by agarose gel electrophoresis, six recombinant colonies were selected and sequenced. …”
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Integrative taxonomy of root-knot nematodes reveals multiple independent origins of mitotic parthenogenesis por Janssen, T., Karssen, G., Topalovic, O., Coyne, Danny L., Bert, W.

“…Consequently, M. decalineata and M. oteifae are synonymized with M. africana, which is herewith redescribed based on results of light and scanning electron microscopy, ribosomal and mitochondrial DNA sequences, isozyme electrophoresis, along with bionomic and cytogenetic features. …”
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Genetic variation, Heritability estimates and GXE effects on yield traits of Mesoamerican common bean (Phaseolus vulgaris L) germplasm in Uganda por Okii, Dennis, Mukankusi, Clare Mugisha, Sebuliba, Sulaiman, Tukamuhabwe, Phineas, Tusiime, Geoffrey, Talwana, Herbert, Odong, Thomas L., Namayanja, Annet, Paparu, Pamela, Nkalubo, Stanley T.

“…Sample genomic DNA was amplified in 2011 with 22 Simple sequence repeat markers (SSRs) and alleles separated using capillary electrophoresis. Field evaluations were conducted in 2010 and 2011 at NaCRRI and 2015 at CIAT – Kawanda. …”
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How does conversion of natural tropical rainforest ecosystems affect soil bacterial and fungal communities in the Nile river watershed of Uganda? por Alele, P.O, Sheil, D., Surget-Groba, Y, Lingling, S, Cannon, Charles H.

“…Despite these differences, no effect on the diversity of dominant taxa for either bacterial or fungal communities was detected, using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Composition of fungal communities did generally appear different in converted sites, but surprisingly, we did not observe a consistent pattern among sites. …”
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Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms por Morón, J. A., Veli Rivera, Eudosio Amancio, Membrillo, A., Paredes, M. M., Gutiérrez Reynoso, Gustavo Augusto

“…DNA was obtained from hair follicles and genotyped for 15 microsatellites markers in multiplex electrophoresis runs.A total of 225 alleles were detected across the 15 loci investigated. …”
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Diversity of Bacillus thuringiensis Strains Isolated from Citrus Orchards in Spain and Evaluation of Their Insecticidal Activity Against Ceratitis capitata por Vidal-Quist, Cristian, Castanera, Pedro, González-Cabrera, Joel

“…SDS-PAGE of spore-crystal preparations revealed 39 different electrophoresis patterns. All primer pairs used in PCR tests gave positive amplifications in strains of our collection, except for primers for detection of cry3, cry19, cry39, or cry44 genes. …”
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Comparative analysis of proteome changes induced by the two spotted spider mite Tetranychus urticae and methyl jasmonate in citrus leaves por Maserti, Bianca E., Del Carratore, R., Della Croce, C. M., Podda, A., Migheli, Q., Froelicher, Yann, Luro, Francois, Morillon, Raphael, Ollitrault, Patrick, Talón, Manuel, Rossignol, M.

“…Although large cDNA microarray profiling has been generated in citrus tissues, the available protein expression data are scarce, in this study, to identify differentially expressed proteins in Citrus clementina leaves after infestation by the two-spotted spider mite Tetranychus urticae, a proteome comparison was undertaken using two-dimensional gel electrophoresis. The citrus leaf proteome profile was also compared with that of leaves treated over 0-72 h with methyl jasmonate, a compound playing a key role in the defense mechanisms of plants to insect/arthropod attack. …”
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Fast detection by loop-mediated isothermal amplification (LAMP) of the three begomovirus species infecting tomato in Panama por Herrera-Vásquez, José A., Puchades, Andrés V., Elvira-González, Laura, Jaén-Sanjur, José N., Carpino, Caterina, Rubio, Luis, Galipienso, Luis

“…Amplification products were visualized by gel electrophoresis or direct Gel-Red staining of DNA into the reaction tube. …”
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Listeria monocytogenes, a food-borne pathogen por Peiris, Inoka

“…Isolated strains were characterized by serotyping and the diversity of the strains within and between producers were determined with PFGE (Pulsed-field gel electrophoresis). The characterized fish isolates were compared with previously characterized human strains. …”
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Milk protein genes polymorphism in indigenous and crossbred cattle from a private dairy farm in Ethiopia por Tewodros, R., Meseret, Selam, Mohammed, S., Dadi, H., Nigussie, H.

“…DNA extraction and quality assessment were done salting out procedure and gel electrophoresis, respectively. Polymerase chain reaction (PCR) was performed with gene specific primers. …”
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