Resultados de búsqueda - "cloning"

Selection of parents in a population hybrid breeding scheme for sweetpotato in Uganda por Swanckaert, J., Ssali, R.T., Mwanga, Robert O.M., Andrade, M.I., Boeck, B. de, Eyzaguirre, R., Campos, Hugo, Grüneberg, W.J.

“…Parents and offspring clones were evaluated in unreplicated trials across five environments during 2018/2019, together with two commercial checks (Ejumula and NASPOT 8). …”
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Pilot study to screen alleles under selection related to drought tolerance in banana por Breton, Catherine, Cenci, Alberto, Roux, Nicolas, Rouard, Mathieu, Carpentier, Sebastien

“…Variability in subgenome contribution to triploid clones makes pertinent to investigate the differential gene expression of Musa cultivars, in particular since genotypes with B subgenomes can be more tolerant to abiotic stresses than those containing only A subgenomes. …”
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Growth and nutrient uptake response of cocoa seedlings (Theobroma cacao) to different growth media at the nursery por Mornyuie, A.O.

“…Seeds of cocoa hybrid clone C69 were sowed into the treatment pots. Cocoa seed germination was recorded at 14 and 17 days after sowing (DAS). …”
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The usefulness of known genes/Qtls for grain quality traits in an indica population of diverse breeding lines tested using association analysis por Zhao, Xiangqian, Zhou, Lijie, Ponce, Kimberley, Ye, Guoyou

“…For these finely mapped QTLs or cloned genes to make an impact in practical breeding, it is necessary to test their effects in different genetic backgrounds. …”
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Understanding Farmer Preferences through Citizen Science: Insights on Potato Varieties in Nigeria por Sharma, K., Atieno, E., Sousa, K. de, Etten, Jacob van, Nyawade, S.

“…Farmer preferences were analyzed using the Plackett-Luce model, a probabilistic model that estimates the relative ‘worth’ (also referred to as log-worth) of each genotype based on observed rankings across multiple comparisons. CIP clones CIP392797.22 and CIP393371.157 emerged as top-ranked, with log-worth values of 0.620 and 0.309, respectively, indicating stronger preference relative to the check. …”
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Developing Molecular Markers Linked to Corky Ringspot Resistance in Solanum tuberosum por Hu, S., Graebner, R., Quick, R., Anglin, Noelle L., Grimm, K.S., Hofferbert, H., Sathuvalli, V.

“…Castle Russet potato variety has shown immunity to TRV and was used in two segregating biparental mapping populations to identify genomic regions linked to CRS resistance. Using 48 clones from the family POR15V001 (Castle Russet x POR08BD1-3), a single marker association analysis for CRS identified a major peak on chromosome 9. …”
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Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction por Masake, R.A., Majiwa, Phelix A.O., Moloo, S.K., Makau, J.M., Njuguna, J., Maina, M., Kabata, J.M., Ole-MoiYoi, O.K., Nantulya, V.M.

“…The antigen is recognized by monoclonal antibody Tv27 currently employed in antigen detection ELISA (Ag-ELISA). A genomic clone which contained a tetramer of the 832-bp cDNA sequence was isolated and shown to be more sensitive than the monomer. …”
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In vitro cultivation of Trypanosoma congolense bloodstream forms in the absence of feeder cell layers por Hirumi, H., Hirumi, K.

“…Bloodstream forms ofTrypanosoma congolense(2 clones: ILNat3·1 and IL3000, and 4 stocks: IL2079, IL2466, IL3266 and CP-81) were continuously cultivatedin vitroat 34–36 °C in the absence of feeder cell layers, using HMI-93 medium which was modified from Iscove's modified Dulbecco's MEM (Flow Laboratories, Irvine, Scotland). …”
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Characterization of Theileria parva which infects water buck (Kobus defassa) por Stagg, D.A., Bishop, Richard P., Morzaria, S.P., Shaw, M.K., Wesonga, D., Orinda, G.O., Grootenhuis, J.G., Molyneux, D.H., Young, A.S.

“…Species-specific synthetic oligonucleotide probes, restriction fragment length polymorphism (RFLP) analysis with cloned T. parva DNA probes, and DNA sequence analysis of the p67 sporozoite antigen gene confirmed that the waterbuck-passaged parasite was T. parva. …”
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Polymorphism in the microsatellite located in the promoters of the Tnfa and Tnfb genes of different mouse strains por Iraqi, F.A., Teale, A.J.

“…Tnfa has also been shown to play an important role in inflammatory immunoregulatory proliferative and antiviral responses and to be important during infection with various protozoan parasites (Beulter and Cerami 1989). Recently we cloned and sequenced the Tnfa genes of three inbred mouse strains. …”
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Evaluation of response to treatment of mixed trypanosome infection in goats using the polymerase chain reaction por Burudi, E.M.E., Peregrine, A.S., Majiwa, Phelix A.O., Murphy, N.B.

“…Three groups of 5 goats each were infected intravenously with either IL 1180 (group A), IL 3274 (group B) or both clones simultaneously (group C), and treated with diminazene aceturate at a does of 7.0 mg/kg b.wt following Development of parasitaemia. …”
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Identification and characterization of iron-related genes in Andean potato cultivars por Hua, X., Boluarte Medina, T., Bonierbale, Merideth W., Williams, M.A., Veilleux, R.E.

“…Additionally, the function of six metal transporter genes cloned from Andean cv. Señora Warmi (CIP 701243), with comparatively greater Fe and Zn content than other Andean cultivars, was investigated by a yeast complementation assay. …”
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The pgip family in soybean and three other legume species: evidence for a birth-and-death model of evolution por Kalunke, R.M., Cenci, A., Volpi, C., O'Sullivan, D.M., Sella, L., Favaron, F., Cervone, F., Lorenzo, G. de, D'Ovidio, R.

“…Merr.) and the characterization of the genomic region surrounding the pgip family in four legume species. Results: BAC clone and genome sequence analyses showed that the soybean genome contains two pgip loci. …”
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Genetic diversity in white- and orange-fleshed sweetpotato farmer varieties from East Africa evaluated by simple sequence repeat markers por Tumwegamire, Silver, Rubaihayo, P.R., LaBonte, D.R., Díaz, F., Kapinga, R., Mwanga, Robert O.M., Grüneberg, W.J.

“…Eighty‐five East African farmer varieties (29 OFSPs and 56 WFSPs) and seven varieties of non‐African origin as check clones were analyzed for diversity using 26 simple sequence repeat (SSR) markers. …”
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Agro-ecological niche of bacterial wilt (Xanthomonas campestris pv. musacearum) of enset (Ensete ventricosum (Welw.) Cheessman) in Gamo highlands of Ethiopia por Sabura, S., Swennen, Rony L., Deckers, J., Aerts, R., Weldeyes, F., Abebe, G., Hailemichael, A., Weldesenbet, F., Blomme, Guy, Vancampenhout, Karen

“…Diverse high yielding clones of different ages are available all year round and can withstand dry spells, making Enset the most important food security crop in these densely populated highlands. …”
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Complete genome sequence of bean leaf crumple virus, a novel begomovirus infecting common bean in Colombia por Carvajal-Yepes, Mónica, Zambrano, Leidy, Bueno, Juan Miguel, Raatz, Bodo, Cuéllar, Wilmer Jose

“…A copy of the complete genome of a novel bipartite begomovirus infecting common bean (Phaseolus vulgaris L.) in Colombia was obtained by rolling-circle amplification (RCA), cloned, and sequenced. The virus is associated with leaf crumple symptoms and significant yield losses in Andean and Mesoamerican beans. …”
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AFLP and AFLPderived SCAR markers associated with Striga gesnerioides resistance in cowpea por Boukar, O., Kong, L., Singh, B.B., Murdock, L., Ohm, H.W.

“…The AFLP fragment from marker combination E-ACT/M-CAC, which is linked in coupling with Rsg1 was cloned, sequenced, and converted into a sequence characterized amplified region (SCAR) marker named SEACTMCAC83/85, which is codominant and useful in breeding programs.…”
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Economic losses experienced by smallscale farmers in Malawi due to cassava brown streak virus disease por Gondwe, F.M.T., Mahungu, N.M., Hillocks, R.J., Raya, M.D., Moyo, C.C., Soko, M.M., Chipungu, Felistus P., Benesi, I.R.M.

“…The paper makes the following recommendations: - the cassava research organisations should mount urgent awareness campaigns of the disease and its management for both extension agents and farmers - the cassava research organisations should establish effective collaboration with the extension system on matters of proper cassava husbandry to effectively and efficiently control the disease - they should additionally carry out local collection exercises for cultivars that show CBSD disease resistance in the high disease pressure areas - there is an urgent need for cassava research and extension organisations to multiply cultivars and promising clones that have shown multiple disease resistance and that are widely accepted by farmers for distribution in the heavily affected areas - in collaboration with entrepreneurs, these organisations need to develop and strengthen sustainable seed multiplication and distribution systems as a way of assuring the provision of clean planting material.…”
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Adapting sweetpotato production to changing climate in Mozambique por Makunde, G.S., Andrade, M.I., Menomussanga, J., Grüneberg, W.J.

“…The objectives of the study were to (i) measure sprouting ability of diverse germplasm of sweetpotato - farmer varieties, improved clones and released varieties and (ii) estimate their ability to provide planting material for the next crop in southern Mozambique. …”
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Variant antigen repertoires in Trypanosoma congolense populations and experimental infections can be profiled from deep sequence data using universal protein motifs por Silva Pereira, S., Casas-Sánchez, A., Haines, L.R., Ogugo, Moses W., Kihara, Absolomon, Sanders, M., Kemp, Stephen J., Acosta-Serrano, Á., Noyes, H., Berriman, M., Jackson, A.P.

“…Periodic switching of the variant surface glycoprotein (VSG) coat covering their cell surface allows sequential expansion of serologically distinct parasite clones. Trypanosome genomes contain many hundreds ofVSGgenes, subject to rapid changes in nucleotide sequence, copy number, and chromosomal position. …”
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