Resultados de búsqueda - "cloning"

Respuesta fotosintética de la yuca (Manihot esculenta Crantz) en dos ambientes por Mejia de Tafur, MS, El-Sharkawy, Mabrouk A., Cantillo, S.H. de

“…Se encontraron diferencias significativas entre clones para cada localidad en las variables de fotosíntesis, conductancia y transpiración. …”
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High-throughput transformation pipeline for a Brazilian japonica rice with bar gene selection por Dedicova, Beata, Bermudez, C., Prias, Monica A., Zuñiga, E, Brondani, C.

“…The following genes of interest: AtNCED3, Lsi1, GLU2, LEW2, PLD-alpha, DA1, TOR, AVP1, and Rubisco were cloned into the binary vector p7i2x-Ubi and were transferred into Agrobacterium strain EHA 105. …”
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Cryopreservation for long-term conservation of cassava genetic resources por Escobar Pérez, Roosevelt H., Mafla, G., Roca, W.

“…At CIAT, an in vitro active gene bank (IVAG) has been established, maintaining under slow growth nearly 5500 clones of the world cultivated and wild Manihot collections. …”
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Genotype by environment effects on potato mini-tuber seed production in an aeroponics system por Mateus-Rodriguez, J., Haan, Stef de, Rodríguez Delfin, A.

“…Both showed stability across different environments for these variables. The advanced clones T3 (395434.1), T5 (397077.16) and T6 (397073.16) showed stability for yield variables, but their performance was below the overall average of the trial. …”
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Removal of leafroll viruses from infected grapevine plants by droplet vitrification por Pathirana, R., McLachlan, A., Hedderley, D., Carra, A., Carimi, F., Panis, Bartholomeus

“…To test the suitability of cryotherapy for virus eradication, we used ‘Chardonnay’ and ‘Lakemont Seedless’ infected with Grapevine leafroll associated virus-3 (GLRaV-3, an Ampelovirus), ‘Pinot gris’ and ‘Sauvignon blanc 316’ infected with Grapevine leafroll associated virus-2 (a Closterovirus), and another clone of ‘Sauvignon blanc’ infected with both Grapevine leafroll associated virus-1 (an Ampelovirus) and GLRaV-3. …”
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PmPPAF is a pro-phenoloxidase activating factor involved in innate immunity response of the shrimp Penaeus monodon por Ma, T.H.T., Benzie, John A.H., Jian-Guo He, Cheng-Bo Sun, Siuming F. Chan

“…In this study, the cDNA encoding a serine proteinase homologue (SPH) with prophenoloxidase activating activity of Penaeus monodon (PmPPAF) was cloned and characterized. PmPPAF cDNA consists of 1444 nucleotides encoding a protein with 394 amino acid residues. …”
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One hundred and six diploids to unravel the genetics of traits in banana: a panel for genome-wide association study and its application to the seedless phenotype por Sardos, J., Rouard, M., Hueber, Y., Cenci, A., Hyma, K., Houwe, Ines van den, Hribova, E., Courtois, Brigitte, Roux, N.

“…We selected a set of 106 diploid accessions with pure M. acuminata background avoiding clone duplicates and generated the appropriate molecular markers to support GWAS for any given trait. …”
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Biological implications in cassava for the production of amylose-free starch: impact on root yield and related traits por Karlström, Amanda, Calle, Fernando, Salazar Erazo, Sandra Milena, Morante, Nelson, Dufour, D.L., Ceballos, Hernán

“…The only significant effect of starch type was on DMC (p < 0.01), with waxy clones having a 0.8% lower content than their wild type counterparts. …”
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Recombinant expression of potentially immunosupressive proteins of the tick vector amblyomma variegatum. por Wang'ombe, G.N.

“…Basic local alignment search tool (BLAST) searches of the AvGI and other tick databases using the p36 sequence identified a total of nine homologues, including four from A. variegatum, with both sequence and length polymorphism.In addition, multiple sequence alignments of these sequences revealed there might be a large family of immunosuppressive proteins (ISPs), perhaps representing a spectrum of immunomodulatory activities.Two putative p36 homologues of A. variegatum, TC183 and TC350, were cloned, sequenced and expressed in recombinant forms. …”
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Evaluation of T cell recognition of theileria parva homologs of apicomplexan antigens. por Kangethe, R.T.

“…Eight T parva homo logs of apicomplexan antigens were sub-cloned into a eukaryotic expression vector and transiently transfected into bovine antigen presenting cells (APC). …”
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Development of a colorimetric assay to assess drug susceptibilities in trypanosoma congolense. por Wasike, R.P.N.

“…The optical density measured at 540 nm was found to increase in direct proportion to the number oftrypanosomes in the range of 5.0xl04 to 8.0x105 organisms 200 sample, The modified assay was tested using seven antitrypanosomal drugs on two stocks and five clones of T. congolense. The trypanocides diminazene aceturate, isometamidium chloride, homidium chloride, quinapyramine sulphate. suramin, melarsoprol and difluoromethyl ornithine (DFMO) were used. …”
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Genotype X Environment interaction of carotene content of yellowfleshed cassava genotypes in Nigeria por Maroya, N., Kulakow, Peter A., Dixon, A., Maziya-Dixon, B.B., Bakare, M.A.

“…Trials of 18 yellow-fleshed genotypes and two officially released white-fleshed clones, used as checks for 2008/2009 and 2009/2010 seasons in five major cassava growing agroecological zones of Nigeria. …”
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Pathogenicity of a 16SrIII-L phytoplasma associated with frogskin disease of cassava (Manihot esculenta Crantz) in Colombia por Álvarez, Elizabeth, Betancourth, Carlos, Muñoz, J.

“…In this study, our goal was to evaluate the pathogenicity of phytoplasma strains isolated from cassava diseased roots. Cassava clones CM 2952, Col 1, Col 896, Bra 184, SM 909-25, Per 334, Per 326, Per 534, and Per 1999, were evaluated. …”
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Differential T-cell responses to a chimeric Plasmodium falciparum antigen; UB05-09, correlates with acquired immunity to malaria por Dinga, J.N., Njimoh, D.L., Kiawa, B., Djikeng, Appolinaire, Nyasa, R.B., Nkuo-Akenji, T., Pelle, Roger, Titanji, V.P.K.

“…UB05, UB09 and UB05-09 were cloned, expressed in E. coli, purified and used to stimulate PBMCs isolated from 63 subjects in a malaria endemic area, for IFN-γ production, which was measured by the ELISpot assay. …”
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Genome-Wide Association Mapping of Correlated Traits in Cassava: Dry Matter and Total Carotenoid Content por Rabbi, Ismail Y., Udoh, L.I., Wolfe, M., Parkes, Elizabeth Y., Gedil, Melaku A, Dixon, A., Ramu, P., Jannink, Jean-Luc, Kulakow, Peter A.

“…A genome-wide association mapping using 672 clones genotyped at 72,279 SNP loci was carried out. …”
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Influence of plant and residue age on attraction, acceptance and larval survival of the banana weevil Cosmopolites sordidus (Coleoptera: Curculionidae) por Masanza, M., Gold, Cliford S., Huis, A. van, Ragama, P.E.

“…In the first experiment, studies focused on different types and ages of residues of one susceptible highland banana clone ‘Nabusa’ (genome group AAA-EA). Corms attracted 65% of the test weevils, pseudostems 30%, while 5% were non-respondents. …”
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Chlorophyll fluorescence analysis for assessing water deficit and Arbuscular Mycorrhizal Fungi (AMF) inoculation in cassava (Manihot esculenta Crantz) por Oyetunji, O., Ekanayake, I.J., Osonubi, O.

“…Field experiment was conducted at Ajibode village while the semi-controlled experiment was conducted at the International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria. One improved cassava clone (TMS 4(2) 1425) and a landrace (TME1) were investigated. …”
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Reproductive efficiency and breeding potential of East African highland (Musa AAAEA) bananas por Ssebuliba, R., Talengera, D., Makumbi, Dan, Namanya, P., Tenkouano, A., Tushemereirwe, Wilberforce K., Pillay, M.

“…Cultivars ‘Entukura’, ‘Enzirabahima’ and ‘Kabucuragye’ of the ‘Nfuuka’ clone set were superior in terms of seed set, presence of seeds with embryos and culturability of embryos. …”
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Meloidogyne spp. and associated galling and damage on cassava in Kenya and Mozambique por Coyne, Danny L., Toko, M., Albanna, L., Hanna, R., Sitole, A., Kagoda, F., Andrade, M.I., Marais, M.

“…In Kenya, M. incognita andM. javanica were identified from severely galled cassava plants from some of the 3710 clones inbreeders’ screening trials. Additional species were also observed but remained unidentified as they did not conform to the specifications of identified species or were too few for positive confirmation. …”
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The occurrence of African cassava mosaic virus and East African cassava mosaic Cameroon virus in natural hosts other than cassava in Nigeria por Alabi, O.J., Ogbe, F.O., Bandyopadhyay, Ranajit, Dixon, Alfred G.O., Hughes, J., Naidu, R.A.

“…In order to confirm the PCR results, we have amplified 1 kbp DNA fragments specific to ACI and AV2 open reading frames (OW) of ACMV and AC3 and ACI ORFs of EACMCV. The amplicons were cloned and nucleotide sequences compared with corresponding sequences of ACMV and EACMCV isolates from cassava. …”
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