Resultados de búsqueda - "cloning"

Evaluation of Genetic Diversity and Genome-Wide Association Studies of Resistance to Bacterial Wilt Disease in Potato por Okiro, L.A., Mulwa, R.M., Oyoo, M.E., Ojwang, P., Otieno, S., Gaiero, P., Pereira, G.S., Mendes, T.

“…Genetic diversity analysis revealed three major clusters whose subgroups were mostly represented by CIP clones derived from common parents. Genome-wide association analyses identified six major hits: two on chromosome 8 and one on each chromosome 2, 4, 5, and 9. …”
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Evaluating a cassava crop growth model by optimizing genotype-specifc parameters using multienvironment trial breeding data por Okoma, P.M., Kayondo, S.I., Rabbi, I.Y., Moreno-Cadena, L.P., Hoogenboom, G., Jannink, J.

“…We parameterized a CGM, the CROPGROMANIHOT- Cassava model in the DSSAT family of models, using data on 67 clones from the International Institute of Tropical Agriculture cassava breeding program evaluated from 2017 to 2020 and over eight locations in Nigeria using trial and error parameter adjustments and the General Likelihood Uncertainty Estimation method. …”
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Genome wide association studies in yam reveal the challenge of high heterozygosity por Agre, A.P., Darkwa, K., Kohtaro, I., Matsumoto, R., Asfaw, A.

“…A total of 173 genotypes including 86 elite breeding clones, 77 genebank accessions, and 10 farmer varieteies were genotyped through whole-genome resequencing, yielding approximately 1.6 million single nucleotide polymorphism (SNP) markers. …”
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Differences in mycorrhizai infection and P uptake of sweet potato cultivars (Ipomoea batatas L.) during their early growth in three soils por Mulongoy, K., Callens, A., Okogun, J.

“…When the low-yielding cultivar, TIb 4, and an improved clone, TIS 9265, were grown in the presence of 50 and 100 mg single superphosphate per kg soil, TIb 4 produced more dry matter in the presence of P fertilizer than it did without the fertilizer. …”
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Effect of exotic tree plantations on plant diversity and biological soil fertility in the Congo savanna: with special reference to eucalypts por Bernhard-Reversat, F.

“…(ii) The relationships between vegetation and fertility are closely linked to litter and soil organic matter quality and quantity these were studied in an age series of eucalypt hybrids and clones. (iii) The density of the main taxa of macrofauna and nematodes was assessed according to the age of plantation and logging. …”
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AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum por Nene, Vishvanath M., Lee, D., Quackenbush, J., Skilton, Robert A., Mwaura, S., Gardner, M.J., Bishop, Richard P.

“…Random clones from a cDNA library made from mRNA purified from dissected salivary glands of feeding female Amblyomma variegatum ticks were subjected to single pass sequence analysis. …”
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Response of diminazene-resistance and diminazene-susceptible Trypanosoma congolense to treatment with diminazene when occuring as a mixed infection in goats por Burudi, E.M.E., Peregrine, A.S., Majiwa, Phelix A.O., Mbiuki, S.M., Murphy, N.B.

“…Three groups of five goats each were infected with IL 1180 (group A.), IL 3274 (group B) or both clones simultaneously (group C), and treated with diminazene aceturate at a dose of 7.0 mg/kg body weight following detection of trypanosomes. …”
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Identification of a bovine surface antigen uniquely expressed on CD4/CD8 negative TCR-gamma/delta positive T lymphocytes por Clevers, H.C., MacHugh, Niall D., Bensaid, A., Dunlap, S., Baldwin, Cynthia L., Kaushal, A., Iams, K.P., Howard, C.J., Morrison, W. Ivan

“…In order to unambiguously define their lineage, cDNA clones encoding bovine T cell receptor (TcR) and CD3 proteins were isolated. …”
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Analysis of erythropoietin and erythropoietin receptor genes expression in cattle during acute infection with Trypanosoma congolense por Suliman, H.B., Logan-Henfrey, L.L., Majiwa, Phelix A.O., Ole-MoiYoi, O.K., Feldman, B.F.

“…Erythropoietin receptor (EpoR) was cloned and sequenced from the two breeds of cattle. …”
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Trypanosoma congolense: High erythropoietic potential in infected yearling cattle during the acute phase of the anemia por Andrianarivo, A.G., Muiya, P., Logan-Henfrey, L.L.

“…To expore the basis for this resistance, the erythropoietic response was evaluated in four naive yearling N'Dama calves and four age-matched Boran calves which developed anemia over a 140-day primary infection with Trypanosoma congolense clone IL 13E3. Similar levels of parasites were detected in the two breeds until 42 days postinfection (dpi). …”
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Structure and function of a novel purine specific nucleoside hydrolase from Trypanosoma vivax por Versees, W., Decanniere, K., Pelle, Roger, Depoorter, J., Brosens, E., Parkin, D.W., Steyaert, J.

“…Nucleoside hydrolases are key enzymes in the purine salvage pathway of Trypanosomatidae, and they are especially attractive because they have no equivalent in mammalian cells. We cloned, expressed and purified a nucleoside hydrolase from Trypanosoma vivax. …”
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Detection of polymorphisms among Theileria parva stocks using repetitive, telomeric ribosomal DNA probes and anti-schizont monoclonal antibodies por Bishop, Richard P., Sohanpal, B.K., Spooner, P.R., Dolan, T.T., Allsopp, B.A., Morzaria, S.P.

“…Many stocks, which were differentiated by the repetitive DNA probe, could not be distinguished using the anti-schizont MAbs. A clonedT. parvasmall subunit ribosomal RNA (SSUrRNA) gene probe separated 17T. parvastocks into 2 groups, exhibiting either 1 or 2 restriction fragments, when hybridized toEcoRI-digestedT. parvaDNA. …”
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Pyroglutamyl peptidase type I from Trypanosoma brucei: A new virulence factor from African trypanosomes that de-blocks regulatory peptides in the plasma of infected hosts por Morty, R.E., Bulau, P., Pelle, Roger, Wilki, S., Abe, K.

“…The enzyme responsible was cloned from a T. brucei brucei genomic DNA library and identified as type IPGP (pyroglutamyl peptidase), belonging to the C15 family of cysteine peptidases. …”
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Identification of bovine cytotoxic T lymphocyte epitopes on the intracellular parasite Theileria Parva por Graham, S.P., Pelle, Roger, Villiers, Etienne P. de, Yamage, M., Mwangi, David Miano, Honda, Y., Ellis, S.A., MacHugh, Niall D., Morrison, W. Ivan, Taracha, E.L.N.

“…The bovine MHC (BoLA) class I alleles that restrict these epitopes were identified by functional screening of BoLA class I cDNA clones and showed that individual alleles restricted five of these peptides and two additional alleles were each found to restrict two peptides. …”
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Feeding behaviour of Glossina pallidipes and G. morsitans centralis on Boran cattle infected with Trypanosoma congolense or T. vivax under laboratory conditions por Moloo, S.K., Sabwa, C.L., Baylis, M.

“…In the first experiment, caged Glossina pallidipes austen were fed for 1 and 5 min on a Boran steer infected with T. congolense clone IL 1180 and on an uninfected steer. Feeding success was recorded in this way five times over several weeks. …”
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Sensitivity and specificity of antigen-capture ELISAs for diagnosis of Trypanosoma congolense and Trypansoma vivax infections in cattle por Eisler, M.C., Lessard, P., Masake, R.A., Moloo, S.K., Peregrine, A.S.

“…Sensitivity and specificity of the FAO/IAEA kits for diagnosis of bovine trypanosomosis were investigated using sera from experimental cattle infected by tsetse challenge with cloned populations of Trypanosoma congolense (three populations) or T. vivax (one population). …”
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Expression of RoTat 1.2 cross-reactive variable antigen type in Trypanosoma evansi and T. equiperdum por Claes, F., Verloo, D., Waal, D.T. de, Urakawa, T., Majiwa, Phelix A.O., Goddeeris, Bruno M., Büscher, Philippe

“…The variable antigen type (VAT) RoTat 1.2 has been cloned from a T. evansi strain, isolated in 1982 from a water buffalo in Indonesia. …”
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Genetic transformation of the tropical forage legume Stylosanthes guianensis with a rice-chitinase gene confers resistance to Rhizoctonia foliar blight disease por Kelemu, Segenet, Changshun, J, Guixi, H, Segura, G.

“…A 1.1-kb rice genomic DNA fragment containing the chitinase gene was cloned into a transformation vector, pCAMBIA2301. …”
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Identifying resistance gene analogs associated with resistances to different pathogens in common beans por López, C.E., Acosta, I.F., Jara, Carlos E., Pedraza García, Fabio, Gaitán Solís, Eliana, Gallego Sánchez, Gerardo J., Beebe, Stephen E., Tohme, Joseph M.

“…A polymerase chain reaction approach using degenerate primers that targeted the conserved domains of cloned plant disease resistance genes (R genes) was used to isolate a set of 15 resistance gene analogs (RGAs) from common bean (Phaseolus vulgaris). …”
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Comparison of simple sequence repeat (SSR) and diversity array technology (DArT) markers for assessing genetic diversity in cassava Manihot esculenta Crantz) por Hurtado López, P.X., Olsen, KM, Buitrago, C., Ospina, C., Marín, J.A., Duque E., Myriam Cristina, Vicente, M. Carmen de, Wongtiem, Prapit, Wenzl, Peter, Killian, A., Adekele, M., Fregene, Martin A.

“…A genome-wide set of 36 SSR markers and a DArT array of approximately 1000 polymorphic clones were used to assess genetic diversity and differentiation. …”
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