Resultados de búsqueda - "cloning"

Genome-wide association analysis for tuber dry matter and oxidative browning in water yam (Dioscorea alata L.) por Gatarira, C., Agre, P., Matsumoto, R., Edemodu, A., Adetimirin, Victor O., Bhattacharjee, Ranjana, Asiedu, R., Asfaw, A.

“…This study used a single nucleotide polymorphism (SNP) assay from a diversity arrays technology (DArT) platform for a genome-wide association study (GWAS) of the two quality traits in a panel of 100 water yam clones grown in three environments. The marker–trait association analysis identified significant SNPs associated with tuber DMC on chromosomes 6 and 19 and with OxB on chromosome 5. …”
Enlace del recurso

Rare instances of haploid inducer DNA in potato dihaploids and ploidy-dependent genome instability por Amundson, K.R., Ordoñez, B., Santayana, M., Ng'ang'a, M.L., Henry, I.M., Bonierbale, Merideth W., Khan, A., Tan, E.H., Comai, L.

“…We characterized the genomes of 919 putative dihaploids and 134 hybrids produced by pollinating tetraploid clones with three HIs: IVP35, IVP101, and PL-4. Whole-chromosome or segmental aneuploidy was observed in 76 dihaploids, with karyotypes ranging from 2n = 2x − 1 = 23 to 2n = 2x + 3 = 27. …”
Enlace del recurso

Incidence of RNA viruses infecting taro and tannia in East Africa and molecular characterisation of dasheen mosaic virusisolates por Kidanemariam, Dawit B., Sukal, A.C., Abraham, Adane D., Njuguna, Joyce N., Stomeo, Francesca, Dale, J.L., James, A.P., Harding, R.M.

“…The complete genomes of nine DsMV isolates from East Africa were cloned and sequenced. Phylogenetic analyses based on the amino acid sequence of the DsMV CP‐coding region revealed two distinct clades. …”
Enlace del recurso

Cytoplasmic Male Sterility Incidence in Potato Breeding Populations with Late Blight Resistance and Identification of Breeding Lines with a Potential Fertility Restorer Mechanism por Santayana, M., Aponte, M., Kante, M., Eyzaguirre, R., Gastelo, M., Lindqvist-Kreuze, H.

“…With the help of pedigree records, reproductive traits evaluations and test crosses with female clones of diverse background, we identified four male parental lines segregating for Rf and three female parental lines that generated 100% male sterile progeny. …”
Enlace del recurso

Variability and genetic merits of white Guinea yam landraces in Nigeria por Agre, A.P., Edemodu, A., Obidiegwu, J., Adebola, P.O., Asiedu, R., Asfaw, A.

“…Methods: A total of 86 white Guinea yam landraces representing the popular landraces in Nigeria alongside 16 elite clones were used for this study. The yam landraces were genotyped using 4,819 DArTseq SNP markers and profiled using key productivity and food quality traits. …”
Enlace del recurso

Escape from CD8+ T Cell Response by Natural Variants of an Immunodominant Epitope from Theileria parva Is Predominantly Due to Loss of TCR Recognition por Connelley, Timothy K., MacHugh, Niall D., Pellé, Roger, Weir, William, Morrison, W. Ivan

“…In this study, we examined the TCR β-chain (TRB) diversity of the CD8+ T cell responses of cattle against two immunodominant epitopes from Theileria parva (Tp1214–224 and Tp249–59) and investigated the role of TCR recognition and MHC binding in determining differential recognition of a series of natural variants of the highly polymorphic Tp249–59 epitope by CD8+ T cell clones of defined TRB genotype. Our results show that both Tp1214–224 and Tp249–59 elicited CD8+ T cell responses using diverse TRB repertoires that showed a high level of stability following repeated pathogenic challenge over a 3-y period. …”
Enlace del recurso

Combined use of sensory methods for the selection of root, tuber and banana varieties acceptable to end-users por Bechoff, A., Adinsi, L., Newilah, G.N., Nakitto, M., Deuscher, Z., Ssali, R.T., Chijioke, Alamu Ugo, Khakasa, E., Nowakunda, K., Bouniol, A., Dufour, D., Bugaud, C.

“…CONCLUSIONS A combined qualitative and quantitative approach involving different sensory techniques is necessary to capture sensory acceptance of products from new RTB clones. Some sensory traits can be correlated to physicochemical characteristics and could be evaluated using laboratory instruments (e.g. texture). …”
Enlace del recurso

Molecular and phenotypic profiling of white Guinea yam (Dioscorea rotundata) breeding lines por Norman, P.E., Asfaw, A., Agre, P.A., Danquah, A., Tongoona, P.B., Danquah, E.Y., Asiedu, R.

“…The genotype–trait (GT) biplot display revealed superior clones with desirable genetic values for the key traits. …”
Enlace del recurso

Genetic variability, characters association and principal component study for morphological and fodder quality of Opuntia and Nopalea sp. in India por Dev, Rahul, Mangalassery, Shamsudheen, Dayal, Devi, Louhaichi, Mounir, Hassan, Sawsan

“…Genotypes, CAZRI-Kukma, Clone 1308, Jalpa, Mexico Unknown, Trunzara Bianca Bronte, Copena F1, CAZRI Botanical Garden, Piantra- 25 and IPA-90-18 are positioned at a wider angle and are more diverse and useful genotypes for use as parent populations for developing new genotypes in future breeding programs.…”
Enlace del recurso

Impact of gender on trait prioritization and food product profile (fufu) development in Nigeria: Guide to breeders por Madu, Tessy, Nwafor, Solomon, Onyemauwa, Nnaemeka, Ukeje, Blesing, Ejechi, Mercy, Okoye, Benjamin

“…Results show that the improved clones compared favorably, or were better, than the local results for roots and fufu mash assessment. …”
Enlace del recurso

Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat por Singh, Charul, Kumar, Ramesh, Sehgal, Hansa, Bhati, Sharmista, Singhal, Tripti, G, Gayacharan, Nimmy, M S, Yadav, Renu, Gupta, Santosh Kumar, Naglaa A, Abdallah, Hamwieh, Aladdin, Kumar, Rajendra

“…Contrary to first generation genome modifying methods, CRISPR/ Cas9 is simple to construct, and clones can hypothetically target several locations in the genome with different guide RNAs. …”
Enlace del recurso

A novel SINE family occurs frequently in both genomic DNA and transcribed sequences in ixodid ticks of the arthropod sub-phylum Chelicerata por Sunter, J.D., Patel, S.P., Skilton, Robert A., Knowles, Donald P., Scoles, Glen A., Nene, Vishvanath M., Villiers, Etienne P. de, Bishop, Richard P.

“…By extrapolation the frequency of occurrence in the randomly selected BAC clone sequences is consistent with approximately 65,000 copies of Ruka in the R. appendiculatus genome. …”
Enlace del recurso

Evaluation and large-scale dissemination of orange-fleshed sweetpotato in SubSaharan Africa por Kapinga, R., Zhang, D., Andrade, M.I., Lemaga, B., Mwanga, R.O.M., Tumwegamire, S., Laurie, S., Ndolo, P.J.

“…Distribution of more than twenty promising clones has been done to Madagascar, Mozambique, Ethiopia, Uganda, Tanzania, Zimbabwe, Egypt, Zanzibar, Malawi, D.R. …”
Enlace del recurso

Gender differentiated utilization of banana as food, medicine and cultural artefact in central Uganda por Mulugo, Lucy, Kyazze, Florence Birungi, Kibwika, Paul, Ngolobe, Emmanuel, Omondi, Bonaventure Aman Oduor, Kikulwe, Enoch

“…The “Nakitembe” cultivar (AAA) emerged as pivotal for food, medicine, and cultural practices, alongside cultivars from the Mbidde clone set. The “Gonja” cultivar (AAB), plantain, held equal significance for food (roasted), medicine, and culture. …”
Enlace del recurso

Effect of clonal integration on drought and waterlogging response in urochloa humidicola por Mayorga, Mildred, Cardoso Arango, Juan Andres

“…The treatments consisted of interactions between three stress conditions applied to the recipient clone: drought, control (no stress), and waterlogging, with two conditions of clonal integration: with integration and without integration (cutting between donor plant and recipient plant). …”
Enlace del recurso

Natural variation of OsML1, a mitochondrial transcription termination factor, contributes to mesocotyl length variation in rice por Meng, Yun, Zhan, Junhui, Liu, Hongyan, Liu, Jindong, Wang, Yamei, Guo, Zhan, He, Sang, Nie, Lixiao, Kohli, Ajay, Ye, Guoyou

“…To date, only a few genes have been cloned, and the mechanisms underlying mesocotyl elongation remain largely unknown. …”
Enlace del recurso

Mapping of tms8 gene for temperature-sensitive genic male sterility (TGMS) in rice (Oryza sativa L.) por Hussain, Appibhai J., Ali, Jauhar, Siddiq, Ebrahimali A., Gupta, Vidya S., Reddy, Umesh K., Ranjekar, Prabhakar K.

“…The 830‐bp RAPD fragment, which segregated with TGMS locus at a distance of 1.33 cM, was eluted and cloned, and sequence information was used for designing sequence‐characterized amplified region (SCAR) primer, which cosegregated with TGMS locus at a distance of 0.8 cM. …”
Enlace del recurso

Influence of different Oryza cultivars on expression of nifH gene pools in roots of rice por Knauth, Stefan, Hurek, Thomas, Brar, Darshan, Reinhold-Hurek, Barbara

“…Phylogenetic analysis of a clone library constructed for nifH fragments expressed in wild species of rice roots indicated that active diazotrophs were not related to cultured strains. …”
Enlace del recurso

AcEXPA1, an α-expansin gene, participates in the aluminum tolerance of carpetgrass (Axonopus compressus) through root growth regulation por Li, Jifu, Liu, Liting, Wang, Linjie, Idupulapati, Rao, Wang, Zhiyong, Chen, Zhijian

“…Among them, a gene encoding α-expansin was cloned and designated AcEXPA1 for functional characterization. …”
Enlace del recurso

Improved Correlation between Instrumental and Sensory Texture of Boiled Sweetpotato por Nakitto, M., Ayetigbo, O., Moyo, M., Ssali, R.T.

“…To compare sensory texture and instrumental texture, sweetpotato roots were harvested from Advanced Yield Trials (AYT) planted in season 2023B from Rwebitaba (n=17) and 2023B Elite clones from Namulonge (n=11). To compare between instrumental texture and consumer acceptability, seven genotypes of contrasting texture were grown and harvested on-station in Namulonge. …”
Enlace del recurso