Resultados de búsqueda - "cloning"

T1-A Cultivo de tejidos: una herramienta valiosa en la conservacion, distribucion e intercambio de germoplasma por Gutiérrez Artunduaga, Janneth Patricia, Ordonez Pena, Carlos Alberto, Cuasialpud Chagueza, Teresa del Carmen, Patino Romero, German Roberto, Bohorquez-Chaux, Adriana, Zhang, Xiaofei, Newby, Jonathan Craig

“…El laboratorio de Cultivo de Tejidos es el punto de apoyo para los diferentes trabajos del área La micropropagación, toma de tejido foliar para extracción de ADN, conservación, y el endurecimiento de vitroplantas en casa de malla, son técnicas que han ayudado al establecimiento de poblaciones mejoradas in vitro para su fácil distribución, conservación, y obtención rápida de clones con el fin de evaluar poblaciones en el programa de Yuca y compartir con países socios.…”
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Identification of diagnostic KASP-SNP markers for routine breeding activities in yam (Dioscorea spp.) por Agre, A.P., Clark, L.V., Garcia-Oliveira, A.L., Bohar, R., Adebola, P.O., Asiedu, R., Terauchi, R., Asfaw, A.

“…Maintaining genetic purity and true-to-type clone identification are important action steps in breeding programs. …”
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Vaccination of cattle with TickGARD induces cross-reactive antibodies binding to conserved linear peptides of Bm86 homologues in Boophilus decoloratus por Odongo, D., Kamau, L., Skilton, Robert A., Mwaura, S., Nitsch, C., Musoke, A.J., Taracha, E.A., Daubenberger, C.A., Bishop, Richard P.

“…The mean weight of engorged female ticks and mean egg mass per tick were significantly reduced in B. decoloratus infestations, but there was no effect of the vaccine against adult Rhipicephalus appendiculatus. We cloned, sequenced and expressed two Bm86 homologues (Bd86) from B. decoloratus. …”
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Genome-wide association study and gene network analyses reveal potential candidate genes for high night temperature tolerance in rice por Bheemanahalli, Raju, Knight, Montana, Quinones, Cherryl, Doherty, Colleen J., Jagadish, Krishna S.V.

“…Positive phenotypic correlations and co-located genetic loci with previously cloned grain size genes revealed common genetic regulation between control and HNT, particularly grain size. …”
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Genome-wide and gene-based association mapping for rice eating and cooking characteristics and protein content por Wang, Xiaoqian, Pang, Yunlong, Zhang, Jian, Wu, Zhichao, Chen, Kai, Ali, Jauhar, Ye, Guoyou, Xu, Jianlong, Li, Zhikang

“…Combining gene-based association study and haplotype analyses plus functional annotation allowed us to shortlist nine candidate genes for four important QTL regions affecting AC, GC and GT, including two cloned genes (Wx and ALK), and seven novels. The research suggested that GWAS and gene-based association analysis followed by haplotype analysis is an effective way to detect candidate genes. …”
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Sweetpotato targeted product profiles alignment to market knowledge by NARS programs in Southern Africa por Makunde, Godwill, Musundire, Lennin, Taula, Antonio, Mtonga, Andrew, Serenje, Gerald, Philime, Memory, Lindqvist-Kreuze, Hannele

“…Pathways to improve collaborations across countries were laid down, and one outcome was sharing of elite clones from International Potato Center breeding program in Mozambique to all countries for further testing and release by national partners.…”
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Epidemiology of bovine trypanosomiasis in the Ghibe valley, southwest Ethiopia. 3. Occurrence of population of Trypanosoma congolense resistant to diminazene, isometamidium and hom... por Codjia, V., Mulatu, W., Majiwa, Phelix A.O., Leak, S.G.A., Rowlands, G.J., Authié, Edith, D'Ieteren, G.D.M., Peregrine, A.S.

“…The drug - sensitivity phenotypes of three of the same isolates were also determined in goats which were each treated with only one of the three trypanocides: all expressed the same phenotypes as the populations expressed in the aforementioned Boran calves. Five clones were derived from one of the isolates which expressed a high level of resistance to all three trypanocides: each alone expressed high levels of resistance to all three trypanocides when characterised in mice. …”
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Apparent rarity of diminazene-resistant trypanosomes in goats infected with a diminazene-resistant population of Trypanosoma congolense por Mamman, M., Williams, D.J.L., Murphy, N.B., Peregrine, A.S.

“…Trypanosoma congolense IL3274, a diminazene-resistant clone, was used to initiate infections in three groups of five goats. …”
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Polymorphic open reading frames encoding secretory proteins are located less than 3 kilobases from Theileria parva telomeres por Bishop, Richard P., Gobright, E.I., Nene, Vishvanath M., Morzaria, S.P., Musoke, A.J., Sohanpal, B.K.

“…Two of the telomere-adjacent ORFs were absent from the genome of a cloned T. parava parasite which can nonetheless be passaged through ticks and cattle. …”
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Concerted evolution at a multicopy locus in the protozoan parasite Theileria parva: Extreme divergence of potential protein-coding sequences por Bishop, Richard P., Musoke, A.J., Morzaria, S.P., Sohanpal, B.K., Gobright, E.I.

“…Herein we show that probes derived from the 5'/N-terminal ends of ORFs in the genomic DNAs of T. parva Uganda (1,108 codons) and Boleni (699 codons) hybridized with multicopy sequences in homologous DNA but did not detect similar sequences in the DNa of 14 heterologous T. parva stocks and clones. The probe sequences were, however, protein coding accoring to predictive algorithms and codon usage. …”
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Establishment of a core cassava germplasm collection in Thailand por Sarakarn, Supachai, Limsila, A, Watananonta, Watana, Thiraporn, C, Sinthuprama, S, Kawano, K

“…In order to provide security of planting material during the establishment period, five test tubes of each clone will be routinely maintained under minimal growth conditions in the laboratory, using Roca`s method. …”
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Genome-wide association and prediction reveals genetic architecture of cassava mosaic disease resistance and prospects for rapid genetic improvement por Wolfe, M.D., Rabbi, Ismail Y., Egesi, Chiedozie N., Hamblin, M., Kawuki, R.S., Kulakow, Peter A., Lozano, R., Carpio, D.P. del, Ramu, P., Jannink, Jean-Luc

“…Finally, we found genomic prediction accuracy of 0.53 to 0.58 suggesting that genomic selection (GS) will be effective both for improving resistance in breeding populations and identifying highly resistant clones as varieties.…”
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Studies on in Vitrro assessment of "drug resistance" in trypanosoma vivax. por Kitosi, D.S.

“…The Long Term Viability Assay and the Drug Incubation Survival Test showed potential to distinguish between isometamidium chloride-sensitive and isometamidium chloride-resistant stocks or clones . of T vivax. Results on drug sensitivity of T vivax stocks obtained with these tests correlated with results in cattle or mice. …”
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Improvement of accession distinctiveness as an added value to the global worth of the yam (Dioscorea spp) genebank por Girma Tessema, G., Korie, S., Dumet, D., Franco, J.

“…Based on a similarity matrix, individual pairs with less than 0.90 similarity coefficients, which varies in six descriptors and more, were considered as distinct and mismatched, whereas those that had similarity coefficients greater than or equal to 0.90 were considered as clones from the same parent. Overall, 20.60% of the total 3156 accessions were found not true to type i.e., misidentified individuals. …”
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Evolution of the banana genome (Musa acuminata) is impacted by large chromosomal translocations por Martin, G., Carreel, F., Coriton, O., Hervouet, C., Cardi, C., Derouault, P., Roques, D., Salmon, F., Rouard, M., Sardos, J., Labadie, Karine, Baurens, F.C., D’Hont, Angélique

“…Most banana cultivars are triploid seedless parthenocarpic clones derived from hybridization between Musa acuminata subspecies and sometimes M. balbisiana. …”
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In vitro methods of germ-plasm conservation por Roca, W., Chavez, R., Martin, M.L., Arias, D.I., Mafla, G., Reyes, Raúl

“…Two types of in vitro gene banks are proposed: (i) an in vitro active gene bank where cultures are maintained under slow growth; of the few of these that exist, the cassava (Manihot) in vitro active gene bank of the Centro Internacional de Agricultura Tropical (CIAT) consists of over 4000 clones; (ii) an in vitro base gene bank, where cultures are cryopreserved; at present, none of this type exist. …”
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Micro-nutrient composition and end-user acceptable quality in potato in Ethiopia por Asfaw, A., Woldegiorgis, G., Kolech, S.A., Mulugeta, G., Mulugeta, D., Ngugi, A., Burgos, G., Felde, T. zum, Bonierbale, Merideth W.

“…The Fe and Zn contents were assessed in peeled tubers of popular farmer varieties, and newly introduced Andean diploid group Phureja accessions and improved tetraploid clones grown in multi-location trials. Grower's preferences for the new potato types was assessed using a participatory approach. …”
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A new virus on maize in Nigeria: maize mild mottle virus por Thottappilly, G., Qiu, W.P., Batten, J.S., Hughes, J.N., Scholthof, K.B.

“…Sequence analyses of a cDNA clone representing a portion of the putative replicase gene aligned most closely with related sequences of viruses within the Tombusviridae. …”
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Theileria parva antigens recognized by CD8+ T cells show varying degrees of diversity in buffalo-derived infected cell lines por Sitt, Tatjana, Pelle, Roger, Chepkwony, M.C., Morrison, W. Ivan, Toye, Philip G.

“…The sequences were derived from parasites in 23 buffalo-derived cell lines, three cattle-derived isolates and one cloned cell line obtained from a buffalo-derived stabilate. …”
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Hierarchical and factorial mating designs in quantitative genetics of tetrasomic potato por Ortíz, R., Golmirzaie, A.

“…Therefore, design I appears to be appropriate for quantitative genetics research in tetrasomic potato, a crop in which some clones are male sterile. The easy handling of crosses (distinct random females included in the crossing scheme) is another advantage of design I relative to design II. …”
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