Resultados de búsqueda - "TIME"

  1. Interference between D and M types of Plum pox virus in Japanese plum assessed by specific monoclonal antibodies and quantitative real-time reverse transcription-polymerase chain reaction por Capote, Nieves, Gorris, María T., Martínez, M. Carmen, Asensio, M., Olmos, Antonio, Cambra, Mariano

    Publicado 2017
    “…Reverse transcription-polymerase chain reaction (RT-PCR) with D- and M-specific primers confirmed the serological typing. Real-time RT-PCR assays were performed using D- and M-specific fluorescent 3′ minor groove binder-DNA probes, which were able to detect and quantify PPV populations in the inoculated plants with greater precision. …”
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  2. Development and evaluation of a one-step multiplex real-time TaqMan® RT-qPCR assay for the detection and genotyping of equine G3 and G14 rotaviruses in fecal samples por Carossino, Mariano, Barrandeguy, Maria Edith, Erol, Erdal, Li, Yanqiu, Balasuriya, Udeni B.R.

    Publicado 2019
    “…Current genotyping methods available for ERVA and rotaviruses affecting other animal species rely on Sanger sequencing and are significantly time-consuming, costly and labor intensive. Here, we developed the first one-step multiplex TaqMan® real-time reverse transcription polymerase chain reaction (RT-qPCR) assay targeting the NSP3 and VP7 genes of ERVA G3 and G14 genotypes for the rapid detection and G-typing directly from fecal specimens. …”
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    Artículo
  3. Discrimination Between Mild and Severe Citrus tristeza virus Isolates with a Rapid and Highly Specific Real-Time Reverse Transcription-Polymerase Chain Reaction Method Using TaqMan LNA Probes por Ruiz-Ruiz, Susana, Moreno, Pedro, Guerri, José, Ambrós, Silvia

    Publicado 2017
    “…Identification of these CTV variants was achieved by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) using a general primer set and three TaqMan locked nucleic acids (LNA) probes targeting sequences characteristic of severe, mild (non-SY, non-SP), and T36-like isolates. …”
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