Resultados de búsqueda - "Reading"

Chromosome-scale genome assembly of the South American fruit fly, Anastrepha fraterculus sp.1 por Rivarola, Maximo Lisandro, Conte, Claudia Alejandra, Berube, Pierre, Chen, Shu-Huang, Giardini, Maria Cecilia, Scannapieco, Alejandra Carla, Milla, Fabian Horacio, Soria, Maria Celeste, Russo, Romina Maria, Wulff, Juan Pedro, Djambazian, Haig H., Pomar, Rolando R., Handler, Alfred M., Bourtzis, Kostas, Ragoussis, Ioannis, Lanzavecchia, Silvia Beatriz

“…Individual female and male samples were sequenced using long- and short-read technologies. The female genome (760 Mb) was de novo assembled into 58 scaffolds and the male genome (750 Mb) into 68 scaffolds, with BUSCO completeness scores of 98.8% and 98.7%, respectively. …”
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RNA-seq analysis of single bovine blastocysts por Chitwood, James L., Rincon, Gonzalo, Kaiser, German Gustavo, Medrano, Juan F., Ross, Pablo J.

“…Approximately 38 million sequencing reads were generated per embryo and 9,489 known bovine genes were found to be expressed, with a high correlation of expression levels between samples (r > 0.97). …”
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Identification of Pomegranate as a New Host of Passiflora Edulis Symptomless Virus (PeSV) and Analysis of PeSV Diversity por Caglayan, Kadriye, Gazel, Mona, Roumi, Vahid, Kocabag, Hamide Deniz, Bahar, Tunç, Reynard, Jean Sebastien, Ruiz-García, Ana B., Olmos, Antonio, Candresse, Thierry

“…Further analysis of HTS reads revealed the presence of five PeSV variants in one of the samples and another single variant in the other. …”
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Whole genome re-sequencing reveals adaptation prior to the divergence of buffalo subspecies por Rafiepour, M., Ebrahimie, E., Vahidi, M.F., Salekdeh, G.H., Niazi, A., Dadpasand, M., Liang, Dong, Si, Jingfang, Ding, Xiangdong, Han Jianlin, Zhang, Yi, Qanbari, S.

“…Our sequencing data reached to a coverage above 99% of the river buffalo reference genome and an average read depth around 9.2× per sample. We identified 20.55 million SNPs, including 63,097 missense, 707 stop-gain, and 159 stop-loss mutations that might have functional consequences. …”
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A comparison of two DNA metagenomic bioinformatic pipelines while evaluating the microbial diversity in feces of Tanzanian small holder dairy cattle por Kibegwa, F.M., Bett, R.C., Gachuiri, C.K., Stomeo, Francesca, Mujibi, F.D.N.

“…However, Kraken 2 generated a more accurate taxonomic identification given the higher number of “Unclassified” reads in MG-RAST. The observed variations at the genus level show that a main restriction is using different databases for classification of the metagenomic data. …”
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Guide to mitigation options to reduce greenhouse gas emissions in Chinese dairy sector por Sha, Wei, Zijlstra, Jelle, Wang, Yue, Hongmin, Dong

“…These paragraphs can be seen as factsheets that can be read independently from each other.…”
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Antibiotic use by poultry farmers in Kiambu County, Kenya: exploring practices and drivers of potential overuse por Kariuki, J.W., Jacobs, J., Ngogang, M.P., Howland, Olivia

“…Findings Of the farmers interviewed, sixty eight percent were female, thirty three percent of the sampled farmers could not read, and the majority (eight five percent) of farmers had reared poultry for at least ten years. …”
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SNP discovery using BSR-Seq approach for spot blotch resistance in wheat (Triticum aestivum L.), an essential crop for food security por Saxesena, Ravi Ranjan, Vinod Kumar Mishra, Chand, Ramesh, Kumar, Uttam, Chowdhury, Apurba Kumar, Bhati, Jyotika, Neeraj Budhlakoti, Joshi, Arun Kumar

“…Transcriptome sequencing of the resistant parent (YS#24), the susceptible parent (YS#58), and their resistant and susceptible bulks yielded a total of 429.67 million raw reads. The bulk frequency ratio (BFR) of SNPs between the resistant and susceptible bulks was estimated, and selection of SNPs linked to resistance was done using sixfold enrichments in the corresponding bulks (BFR >6). …”
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Terms of Reference: CGIAR Science Group Evaluations por CGIAR Independent Advisory and Evaluation Service

“…The evaluation Terms of Reference (TORs), which are endorsed by the Strategic Impact, Monitoring, and Evaluation Committee (SIMEC) and build on the Concept Note, are presented to the CGIAR System Council (SC19) as a pre-read. The SG evaluations align with CGIAR's Evaluation Framework and Policy, and stem from the CGIAR 2030 Research and Innovation Strategy. …”
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Canine Staphylococcaceae circulating in a Kenyan animal shelter por Akarsu, H., Liljander, Anne M., Lacasta, Anna, Ssajjakambwe, P., Brodard, I., Cherbuin, J.D.R., Torres-Puig, S., Perreten, V., Kuhnert, P., Labroussaa, F., Jores, Joerg

“…In all, 92 strains were isolated from 85 dogs and subsequently sequenced by PacBio long-read sequencing. Strains encompassed nine validated species, while <i>S. aureus</i> (n = 47), <i>S. pseudintermedius</i> (n = 21), and <i>Mammaliicoccus (M.) sciuri</i> (n = 16) were the three most dominant species. …”
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Design, construction and evaluation of a drone-tethered river survey vessel por Maharaj, U., Singh, K., Pike, T., Pike, C.

“…The vessel that was built was equipped with temperature sensors to capture thermal variations across a section of the river, a Total Dissolved Solids (TDS) sensor to monitor total dissolved solids, a Sound Navigation and Ranging (SoNAR) device to map variations in the riverbed profile, a turbidity sensor to provide a measure of water clarity and a Global Positioning System (GPS) module for precise geotagging of all sensor readings. The system was tested at the Lions River in KwaZulu-Natal, South Africa, and the drone-tethered data collection vessel enabled a safe and efficient survey of a river segment. …”
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The role of multi-stakeholder forums in subnational jurisdictions: Methods training manual and tools for in-depth research por Sarmiento Barletti, Juan Pablo, Larson, Anne M.

“…This manual should be read in tandem with the project s Framing literature review for in-depth field research. …”
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Exploiting interspecific conservation of male-specific genomic regions to develop markers for sex determination in catla (Labeo catla) and silver carp (Hypophthalmichthys molitrix) por Hamilton, Matthew G., Akhter, Md. Masud, Shanta, Sirajum Monira, Roy, Aashish Kumar, Rahman, Md. Mustafizur, Sarkar, Uzzal Kumar, Kamruzzaman, Md., Benzie, John A.H.

“…These markers were incorporated into DArTag panels and evaluated, according to read-count thresholds, in individuals of known visually-assessed or functional (i.e. reproductive) phenotypic sex (PSEX). …”
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Exploiting interspecific conservation of male-specific genomic regions to develop markers for sex determination in catla (Labeo catla) and silver carp (Hypophthalmichthys molitrix) por Hamilton, Matthew, Akhter, Md, Shanta, Sirajum Monira, Roy, Aashish Kumar, Rahman, Md. Mustafizur, Sarkar, Uzzal Kumar, Kamruzzaman, Md., Benzie, John

“…These markers were incorporated into DArTag panels and evaluated, according to read-count thresholds, in individuals of known visually-assessed or functional (i.e. reproductive) phenotypic sex (PSEX). …”
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Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae) por Ouma, J.O., Masake, R.A., Masiga, Daniel K., Moloo, S.K., Njuguna, J.T., Ndung'u, J.M.

“…A visually read dot-enzyme linked immunosorbent asay (dot-ELISA) developed for the detection of trypanosomes in tsetse flies (Glossina spp.) was evaluated in the laboratory and under field conditions. …”
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A study of domestic pigs, wilds suids and ticks as reservoirs for African swine fever virus in Uganda por Bjornheden, L.

“…Three of the PCR positive pigs were borderline cases on the ELISA. GPS readings of the movement of the bushpigs indicated possible interface with the domestic pigs.Conclusions made from this study are that domestic pigs, bushpigs, warthogs and soft ticks can act as reservoirs for ASFV. …”
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Field-applicable recombinase polymerase amplification assay for rapid detection of Mycoplasma capricolum subsp. Capripneumonia por Liljander, Anne M., Mingyan Yu, O'Brien, E., Heller, M., Nepper, J.F., Weibel, D.B., Gluecks, Ilona V., Younan, M., Frey, J., Falquet, Laurent, Jores, Joerg

“…We demonstrate that the diagnosis of CCPP can be achieved, with a short sample preparation time and a simple read-out device that can be powered by a car battery, in <45 min in a simulated field setting.…”
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Transcriptomic profiling in Musa: a look into processes affected by mild osmotic stress in the root tip por Zorrilla-Fontanesi, Yasmín, Rouard, M., Cenci, A., Kissel, E., Do, H., Dubois, E., Nidelet, S., Roux, N., Swennen, Rony L., Carpentier, Sebastien C.

“…In total, 18 cDNA libraries were sequenced producing around 568 million high quality reads, of which 70-84% were mapped to the diploid reference genome (D’Hont et al., 2012). …”
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Determinants of the utilization of desho grass (Pennisetum pedicellatum) by farmers in Ethiopia por Asmare, Bimrew, Demeke, S., Ejeta, Taye Tolemariam, Tegegne, Firew, Wamatu, Jane, Rischkowsky, Barbara A.

“…Although the majority of respondents can read and write, about 23% of respondents were illiterate. …”
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Deep sequencing of Suppression Subtractive Hybridisation drought and recovery libraries of the non-model crop Trifolium repens L. por Bisaga, M., Lowe, M., Hegarty, M., Abberton, Michael T., Ravagnani, A.

“…We created a white clover reference transcriptome of 16,193 contigs by deep sequencing (mean base coverage 387x) four Suppression Subtractive Hybridization (SSH) libraries (a forward and a reverse library for each treatment) constructed from young leaf tissue of white clover at the onset of the response to drought and recovery. Reads from individual libraries were then mapped to the reference transcriptome and processed comparing expression level data. …”
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