Resultados de búsqueda - "Proteomics"

Matrix-assisted laser desorption/ionization time of flight mass spectrometry for comprehensive indexing of East African Ixodid tick species por Rothen, J., Githaka, Naftaly W., Kanduma, Esther G., Olds, Cassandra L., Pflueger, V., Mwaura, S., Bishop, Richard P., Daubenberger, C.A.

“…The objective was to determine the utility of this proteomic tool for reliable species identification of closely related afrotropical ticks.…”
Enlace del recurso

Identification of resistanceassociated proteins in closelyrelated maize lines varying in aflatoxin accumulation por Chen, Z., Brown, R.L., Menkir, A., Cleveland, T.E.

“…Five pairs of closely-related lines that had 75–94% genetic similarity within each pair and which varied within each pair in aflatoxin accumulation were selected for proteomic investigation. Kernel embryo and endosperm protein profile differences within the pair and across pairs were compared using two-dimensional polyacrylamide gel electrophoresis. …”
Enlace del recurso

The ÓMICAS alliance, an international research program on multi-omics for crop breeding optimization por Jaramillo Botero, Andres, Colorado, Julian D., Quimbaya, Mauricio, Rebolledo, María Camila, Lorieux, Mathias, Ghneim-Herrera, Thaura, Arango, Carlos A., Tobón, Luis E., Finke, Jorge, Rocha, Camilo, Muñoz, Fernando, Riascos, John J., Silva, Fernando, Chirinda, Ngonidzashe, Caccamo, Mario Jose, Vandepoele, Klaas, Goddard, William A.

“…The strategy and methods described in this article, involve the characterization of different crop models, using high-throughput, real-time phenotyping technologies as well as experimental tissue characterization at different levels of the omics hierarchy and under contrasting conditions, to elucidate epigenome-, genome-, proteome- and metabolome-phenome relationships. The massive data sets are used to derive in-silico models, methods and tools to discover complex underlying structure-function associations, which are then carried over to the production of new germplasm with improved agricultural traits. …”
Enlace del recurso

First draft genome assembly of the Peruvian creole cattle breed (Bos taurus) and its comparative genomics among the Bovinae subfamily por Estrada Cañari, Richard, Corredor Arizapana, Flor Anita, Figueroa Venegas, Deyanira Antonella, Salazar Coronel, Wilian, Quilcate Pairazamán, Carlos Enrique, Vásquez Pérez, Héctor Vladimir, Maicelo Quintana, Jorge Luis, Gonzales, Jhony, Arbizu Berrocal, Carlos Irvin

“…A total of 19,803 protein-coding genes were annotated in the PCC genome. We downloaded proteomes and genomes of the Bovinae subfamily, and conducted a comparative analysis with our draft genome. …”
Enlace del recurso
Enlace del recurso

High antibody titres against predicted Mycoplasma surface proteins do not prevent sequestration in infected lung tissue in the course of experimental contagious bovine pleuropneumo... por Schieck, Elise, Liljander, Anne M., Hamsten, Carl, Gicheru, Nimmo, Scacchia, Massimo, Sacchini, Flavio, Heller, Martin, Schnee, Christiane, Sterner-Kock, Anja, Hlinak, Andreas, Naessens, Jan, Poole, Elizabeth J., Persson, Anna S., Jores, Joerg

“…Interestingly we found that animals that developed Mycoplasma-containing sequestra had significantly higher antibody levels against proteins comprising the surface proteome than the animals that cleared Mycoplasma from their lungs. …”
Enlace del recurso

Selective pressure against horizontally acquired prokaryotic genes as a driving force of plastid evolution por Llorente, Briardo, de Souza, Flavio S. J., Soto, Gabriela Cynthia, Meyer, Cristian, Alonso, Guillermo D., Flawia, Mirtha M., Bravo Almonacid, Fernando Felix, Ayub, Nicolás Daniel, Rodríguez-Concepción, Manuel

“…Our results reveal a possible evolutionary mechanism of how selection against heterologous genes encoding cytosolic proteins contributed in incrementing plastid proteome complexity from non-endosymbiotic gene sources, a process that may also impact mitochondrial evolution.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

BioMoby extensions to the Taverna workflow management and enactment software por Kawas, Edward, Senger, Martin, Wilkinson, Mark D.

“…As biology becomes an increasingly computational science, it is critical that we develop software tools that support not only bioinformaticians, but also bench biologists in their exploration of the vast and complex data-sets that continue to build from international genomic, proteomic, and systems-biology projects. The BioMoby interoperability system was created with the goal of facilitating the movement of data from one Web-based resource to another to fulfill the requirements of non-expert bioinformaticians. …”
Enlace del recurso

Tripal, a community update after 10 years of supporting open source, standards-based genetic, genomic and breeding databases por Staton, Margaret, Cannon, Ethalinda K.S., Sanderson, Lacey-Anne, Wegrzyn, Jill, Anderson, Tavis, Buehler, Sean, Cobo-Simón, Irene, Faaberg, Kay, Grau, Emily, Guignon, Valentin, Gunoskey, Jessica, Inderski, Blake, Jung, Sook, Lager, Kelly, Main, Dorrie, Poelchau, Monica F., Ramnath, Risharde, Richter, Peter, West, Joe, Ficklin, Stephen

“…With increasing volumes, complexity and the need to integrate genomic, transcriptomic, metabolomic, proteomic, phenomic and environmental data, community databases face tremendous challenges in ongoing maintenance, expansion and upgrades. …”
Enlace del recurso

Stress response and detoxification mechanisms involved in non-target-site herbicide resistance in sunflower por Vega, Tatiana, Gil, Mercedes, Martin, Gabriela, Moschen, Sebastian Nicolas, Picardi, Liliana, Nestares, Graciela

“…Roots were collected, and label-free quantitation (LFQ) proteomic analysis was carried out to characterize the NTSR mechanisms involved in the IMI resistance trait in sunflower. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

Proteins Related to Stress and Redox State of 'Moncada' Mandarin Leaves with Contrasting Fruit Load por Munoz-Fambuena, Natalia, Mesejo, Carlos, Agustí, Manuel, Tarraga, Susana, Iglesias, Domingo J., Primo-Millo, Eduardo, González-Mas, María C.

“…A proteomic study was used to know more about the Citrus redox state and stress response related to fruit load. …”
Enlace del recurso

A USA Africa collaborative strategy for identifying, characterising and developing maize germplasm with resistance to aflatoxin contamination por Menkir, A., Brown, R.L., Bandyopadhyay, Ranajit, Chen, Z.Y., Cleveland, T.E.

“…These pairs of lines are currently being used for proteome analysis to identify resistance-associated proteins and the corresponding genes underlying resistance to aflatoxin accumulation. …”
Enlace del recurso

Gut-expressed vitellogenin facilitates the movement of a plant virus across the midgut wall in its insect vector por He, Ya-Zhou, Wang, Yu-Meng, Yin, Tian-Yan, Cuéllar, Wilmer Jose, Liu, Shu-Sheng, Wang, Xiao-Wei

“…Here, in order to identify whitefly midgut proteins that interact with a devastating begomovirus, tomato yellow leaf curl virus (TYLCV), we performed midgut-specific TYLCV coat protein (CP) immunoprecipitation followed by high-throughput mass spectrometry proteomic analysis. We find that vitellogenin (Vg), a critical insect reproductive protein that has been considered to be synthesized by the fat body, is also synthesized by and interacts with TYLCV CP in the whitefly midgut. …”
Enlace del recurso

Post-translational signal peptide cleavage controls differential epitope recognition in the QP-rich domain of recombinant Theileria parva PIM por Casanova, C.L., Xue, G., Taracha, E.L.N., Dobbelaere, D.A.E.

“…A recent analysis of the proteomes of T. parva and T. annulata revealed the presence of a large family of potentially secreted proteins, characterized by the presence of large stretches of amino acids that are also particularly rich in QP-residues.…”
Enlace del recurso

Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology por Tomazic, Mariela Luján, Rodriguez, Anabel Elisa, Lombardelli Joaquín Andrés, Poklepovich Caride, Tomás Javier, Garro, Carlos Javier, Galarza, Roxana Ivon, Tiranti, Karina, Florin-Christensen, Mónica, Schnittger, Leonhard

“…A reverse vaccinology approach was employed to (i) identify the GPI-anchored proteome of C. parvum using available web-based bioinformatic tools and (ii) characterize previously unrecognized novel vaccine antigens. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

Next-generation biological control: the need for integrating genetics and genomics por Leung, Kelley, Ras, Erica, Ferguson, Kim B., Arïens, Simone, Babendreier, Dirk, Bijma, Piter, Bourtzis, Kostas, Brodeur, Jacques, Bruins, Margreet A., Centurión, Alejandra, Chattington, Sophie R., Chinchilla-Ramírez, Milena, Dicke, Marcel, Fatouros, Nina E., González-Cabrera, Joel, Groot, Thomas V. M., Haye, Tim, Knapp, Marcus, Koskinioti, Panagiota, Le Hesran, Sophie, Lyrakis, Manolis, Paspati, Angeliki, Pérez-Hedo, Mertixell, Plouvier, Wouter N., Schlötterer, Christian, Stahl, Judith M., Thiel, Andra, Urbaneja, Alberto, Van-De-Zande, Louis, Verhulst, Eveline C., Vet, Louise E. M., Visser, Sander, Werren, John H., Xia, Shuwen, Zwaan, Bas J., Magalhaes, Sara, Beukeboom, Leo W., Pannebakker, Bart A.

“…Gene discovery involves quantitative trait loci analyses, transcriptomic and proteomic studies, and gene editing. Improving biocontrol practices includes marker‐assisted selection, genomic selection and microbiome manipulation of biocontrol agents, and monitoring for genetic variation during rearing and post‐release. …”
Enlace del recurso
Enlace del recurso

Insights into microbially induced salt tolerance and endurance mechanisms (stem) in plants por Kaushal, M.

“…Molecular research in MI plants under salt stress conditions has found variations in the expression profiles of genes such as HKT1, NHX, and SOS1 (ion transporters), PIPs and TIPs (aquaporins), RBCS, RBCL (RuBisCo subunits), Lipoxygenase2 [jasmonic acid (JA) signaling], ABA (abscisic acid)-responsive gene, and APX, CAT, and POD (involved in antioxidant defense). Proteomic analysis in arbuscular mycorrhizal fungi-inoculated plants revealed upregulated expression of signal transduction proteins, including Ca2+ transporter ATPase, calcium-dependent protein kinase, calmodulin, and energy-related proteins (NADH dehydrogenase, iron-sulfur protein NADH dehydrogenase, cytochrome C oxidase, and ATP synthase). …”
Enlace del recurso

Designing bovine T cell vaccines via reverse immunology por Nene, Vishvanath M., Svitek, Nicholas, Toye, Philip G., Golde, W.T., Barlow, J., Buus, Soren, Nielsen, Morten

“…When retrained, NetMHCpan may be used to predict parasite peptide epitopes by scanning the predicted T. parva proteome and known parasite CTL antigens. A range of pMHC tetramers, made “on-demand”, will then be used to assay cattle that are immune to ECF or in vaccine trials to determine if CTLs of the predicted epitope specificity are present or not. …”
Enlace del recurso

Evaluation of cross-protection of African swine fever vaccine ASFV-G-ΔI177L between ASFV biotypes por Borca, M.V., Ramirez-Medina, E., Mutisya, Christine, Ojuok, Rose, Odaba, Josiah, Dihbol, M., Lacasta, Anna, Gladue, D.P.

“…Biotyping, considering the entire virus proteome, appears to be a more promising prediction tool, although additional gathering of experimental data will be necessary to fully validate it; until then, the presence of cross-protection needs to be confirmed in efficacy trials challenging vaccinated animals.…”
Enlace del recurso

A Babesia bovis gene syntenic to Theileria parva p67 is expressed in blood and tick stage parasites por Freeman, J.M., Kappmeyer, L.S., Ueti, M.W., McElwain, T.F., Baszler, T.V., Echaide, I., Nene, Vishvanath M., Knowles, Donald P.

“…Completion of the Babesia bovis (T2Bo strain) genome provides detailed data concerning the predicted proteome of this parasite, and allows for a bioinformatics approach to gene discovery. …”
Enlace del recurso

Breeding maize for resistance to mycotoxins at IITA por Menkir, A., Brown, R.L., Bandyopadhyay, Ranajit, Chen, Z.Y., Cleveland, T.E.

“…These pairs of lines are being used for proteomic analyses to identify the proteins and the corresponding genes that limit aflatoxin accumulation. …”
Enlace del recurso