Search Results - "Proteomics"

  1. Evaluation of four different strategies to characterize plasma membrane proteins from banana roots by García, S., Panis, Bartholomeus, Swennen, Rony L., Carpentier, Sebastien C.

    Published 2014
    “…In this work, we evaluated four different strategies for the characterization of plasma membrane proteins from banana roots: (i) the aqueous-polymer two-phase system technique (ATPS) coupled to gelelectrophoresis (gel-based), and (ii) ATPS coupled to LC-MS/MS (gel free), (iii) a microsomal fraction and (iv) a full proteome, both coupled to LC-MS/ MS. Our results show that the gel-based strategy is useful for protein visualization but has major limitations in terms of time reproducibility and efficiency. …”
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    Journal Article
  2. Carotenoid composition and sequestration in cassava (Manihot esculentum Crantz) roots by Drapal, Margit, Ovalle Rivera, Tatiana Melissa, Becerra Lopez Lavalle, Luis Augusto, Fraser, Paul D.

    Published 2024
    “…Carotenoids were associated with fractions typically annotated as plastoglobuli and plastid membranes (particularly the envelope). Proteomic analysis confirmed these structures apart from plastoglobuli, thus potentially amyloplast structures may not contain classical plastoglobuli structures.…”
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    Journal Article
  3. Comparisons of protein profiles of beech bark disease resistant and susceptible American beech (Fagus grandifolia) by Mason, M.E., Koch, J.L., Krasowski, M., Loo, J.

    Published 2013
    “…The disease begins when exotic beech scale insects feed on the bark of trees, and is followed by infection of damaged bark tissues by one of the Neonectria species of fungi. Proteomic analysis was conducted of beech bark proteins from diseased trees and healthy trees in areas heavily infested with beech bark disease. …”
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    Journal Article
  4. Orbitrap mass spectrometry as an antibody validation method by Ramezani, Mehrafarin

    Published 2015
    “…Over a decade after its start, Human Protein Atlas (HPA) has been developed as a universal database for human proteome. HPA has used both commercially designed and intra-designed antibodies for protein detection but both types should pass several validation tests like Western blot, immunohistochemistry, protein array and immunofluo-rescence. …”
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  5. Heat treatment of peach fruit: Modifications in the extracellular compartment and identification of novel extracellular proteins by Bustamante, Claudia Anabel, Budde, Claudio Olaf, Borsani, Julia, Lombardo, Verónica Andrea, Lauxmann, Martin Alexander, Andreo, Carlos Santiago, Lara, María Valeria, Drincovich, María Fabiana

    Published 2024
    “…In the present work, the levels of twelve transcripts encoding proteins involved in cell wall metabolism, as well as the differential extracellular proteome, were examined after a post-harvest heat treatment (HT; 39 °C for 3 days) of “Dixiland” peach fruit. …”
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    Artículo
  6. Molecular signatures of silencing suppression degeneracy from a complex RNA virus by Gómez-Munoz, Neus, Gimenez-Santamarina, Silvia, Sanchez-Vicente, Javier, Navarro-López, Josep, Martínez, Fernando, Daros, José A., Rodrigo, Guillermo

    Published 2021
    “…In the present study, we followed a proteomic approach together with a computational network analysis to reveal molecular signatures of protein function degeneracy from a plant virus (as virus-host protein-protein interactions). …”
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    Artículo
  7. Fruit growth and development by Tadeo, Francisco R., Terol, Javier, Rodrigo, María J., Licciardello, Concetta, Sadka, Avi

    Published 2020
    “…The chapter incorporates updated knowledge on citrus fruit growth and development acquired both by classical agronomic, genetic, and physiological means, and more recent studies using genomic, proteomic, and metabolomic approaches.…”
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    Capítulo de libro
  8. Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets by Gismondi, Mauro, Strologo, Laura, Gabilondo, Julieta, Budde, Claudio Olaf, Drincovich, María Fabiana, Bustamante, Claudia Anabel

    Published 2025
    “…Besides, comparing the DEPs between Arabidopsis plants expressing PpZAT12 or AtZAT12 (35S::AtZAT12) we found common and specific responses to these TFs. Based on the proteomic information obtained here and published data about AtZAT12, we searched ZAT12-targets in peach allowing the identification of a putative ZAT12 regulon in this species. …”
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    Artículo
  9. Toward community standards in the quest for orthologs by Dessimoz, C., Gabaldon, T., Roos, D.S., Sonnhammer, E., Herrero, J., Quest for Ortholog Consortium

    Published 2012
    “…Yet, the development and application of orthology inference methods is hampered by the lack of consensus on source proteomes, file formats and benchmarks. The second ‘Quest for Orthologs’ meeting brought together stakeholders from various communities to address these challenges. …”
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    Journal Article
  10. Rice responds to endophytic colonization which is independent of the common symbiotic signaling pathway by Chen, Xi, Miché, Lucie, Sachs, Sabrina, Wang, Qi, Buschart, Anna, Yang, Haiyuan, Vera Cruz, Casiana M., Hurek, Thomas, Reinhold-Hurek, Barbara

    Published 2015
    “…Putative homologs of genes required for the CSP were analyzed for their putative role in endophytic colonization. Proteomic and suppressive subtractive hybridization (SSH) approaches were also applied, and a comparison of defense‐related processes was carried out by setting up a pathosystem for flooded roots with Xanthomonas oryzae pv. oryzae strain PXO99 (Xoo). …”
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    Journal Article
  11. The Mn-binding proteins of the photosystem II oxygen-evolving complex are decreased in date palms affected by brittle leaf disease by Marques, Jorge, Durán-Vila, Núria, Daros, José A.

    Published 2017
    “…The disease is specifically associated with an increase in a series of chloroplastic RNAs. A proteomic analysis of leaflets of affected and unaffected date palms showed differences in quantities of several proteins. …”
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    Artículo
  12. Linking genotype to phenotype: the International Rice Information System (IRIS) by Bruskiewich, Richard M., Cosico, Alexander B., Eusebio, William, Portugal, Arllet M., Ramos, Luralyn M., Reyes, Ma. Teresa, Sallan, May Ann B., Ulat, Victor Jun M., Wang, Xusheng, McNally, Kenneth L., Hamilton, Ruaraidh Sackville, McLaren, Christopher Graham

    Published 2003
    “…Building upon the germplasm genealogy and field data components of ICIS, IRIS is being extended to handle diverse rice genomics data including: genetic mapping, genome annotation, genotype, mutant, transcripteome, proteome and metabolomic data. Users can access information in the database through stand-alone programs and WWW interfaces offering specialist views to researchers with different interests.Availability: http://bioinformatics.org/project/?…”
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    Journal Article
  13. Bioinformatics for plant genetics and breeding research by Naik, Y.D., Zhao, C., Channale, S., Nayak, S.N., Bhutia, K.L., Gautam, A., Kumar, R., Niranjan, V., Shah, T.M., Mott, R., Punnuri, S., Pandey, M.K., Wang, X., Varshney, R.K., Thudi, M.

    Published 2024
    “…As a result, there has been an increasing demand for statistical and bioinformatics tools to analyse and manage the enormous amount of data generated from sequencing of genomes, transcriptomes, proteome and metabolomes. In this chapter, we review the intervention of bioinformatics and computational tools for deploying the tremendous wealth of data for plant genetics and breeding research.…”
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    Book Chapter
  14. Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins by Kotsovolos, Nikolaos, Schnittger, Leonhard, Sibeko-Matjila, Kgomotso

    Published 2025
    “…Furthermore, the localization of these 188 proteins was investigated, resulting in their assignment to seven cellular compartments. Screening of the proteomes of other Theileria species, T. annulata, T. orientalis, and T. equi revealed that all 188 proteins were present in both transforming and non-transforming Theileria parasites. …”
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    Artículo

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