Resultados de búsqueda - "PBS"

Inducción de la ovulación en llamas mediante la administración intramuscular del plasma seminal de llama, alpaca y toro por López F., Alicia, Huanca López, Wilfredo, Leyva V., Víctor, Falcón P., Néstor, Huanca Mamani, Teodosio, Ratto F., Marcelo

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Efecto de fracciones del plasma seminal, según su peso molecular, sobre la inducción de la ovulación en llamas (Lama glama) por Vásquez E., Martha, Huanca L., Wilfredo, Huanca Mamani, Teodosio, Ratto F., Marcelo, Adams, Gregg Patrick

“…Los animales se distribuyeron al azar a uno de los seis tratamientos: A) fracción de plasma seminal con PM >30 kDa, B) fracción de plasma seminal con PM entre 10 y 30 kDa, C) fracción de plasma seminal con PM entre 5 y 10 kDa, D) fracción de plasma seminal con PM <5 kDa, E) plasma seminal completo, y F) PBS. Los animales fueron tratados con una solución de 1.5 ml de la fracción de plasma seminal o placebo correspondiente por vía intramuscular. …”
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Diagnosis of Xanthomonas axonopodis pv. citri, causal agent of citrus canker, in commercial fruits by isolation and PCR-based methods por Golmohammadi, Morteza, Cubero, Jaime, Penyalver, Jaime, Quesada, Jose M., López, María M., Llop, Pablo

“…Canker-like lesions were disrupted in PBS buffer, and the extract used for bacterial isolation and DNA extraction followed by PCR amplification. …”
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An estimation of thermophilic Campylobacter population in ready-to-eat roast beef and chicken sold and hygiene practices of sellers in beer bars in Arusha, Tanzania. por Toyomaki, H., Ishihara, K., Sanka, P., Kurwijila, Lusato R., Grace, Delia, Makita, K.

“…Fifty grams of samples were rinsed with 25 ml of Phosphate Buffered Saline (PBS) and one ml of each three replicates of this solution and 10 and 100 times diluted solutions were inoculated to Preston broth and incubated at 42°C for 24 hours in a CO2 jar. …”
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Algunas Características de los Embriones Colectados de Vicuña (Vicugna vicugna) en el CIP Quimsachata del INIA PUNO por Cárdenas Minaya, Oscar Efraín, Sapana, R., Gonzáles Castillo, Mario Lino, Mamani Cato, Rubén Herberht

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Preparation and characterization of a Staphylococcus aureus capsular polysaccharide-protein conjugate prepared by a low cost technique: a proof-of-concept study por Pujato, Nazarena, Díaz, Germán, Barbagelata, Maria Sol, Vicco, Miguel Hernán, Calvinho, Luis Fernando, Marcipar, Ivan Sergio

“…Mice groups were immunized at days 0, 14, 28, and 42, with the conjugate (CP5-HSAPBQ), free CP5, or PBS, formulated with incomplete Freund adjuvant, and after 3 months, they were challenged with free CP5 to evaluate the memory response. …”
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Transcriptome profile of spleen tissues from locally-adapted Kenyan pigs (Sus scrofa) experimentally infected with three varying doses of a highly virulent African swine fever viru... por Machuka, Eunice M., Juma, John, Muigai, A.W.T., Amimo, J.O., Pelle, Roger, Okoth, Edward A.

“…Methods Locally adapted pigs (n = 14) were experimentally infected with a high dose (1x106HAD50), medium dose (1x104HAD50), and low dose (1x102HAD50) of the highly virulent genotype IX ASFV Ken12/busia.1 (Ken-1033) isolate diluted in PBS and followed through the course of infection for 29 days. …”
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An oral salmonella-based vaccine inhibits liver metastases by promoting tumor-specific T-cell-mediated immunity in celiac and portal lymph nodes : a preclinical study por Vendrell, Alejandrina, Mongini, Claudia, Gravisaco, Marí­a José, Canellada, Andrea, Tesone, Agustina Inés, Goin, Juan Carlos, Waldner, Claudia Inés

“…To this end, one group of BALB/c mice was orogastrically immunized with CVD 915, while another received PBS as a control. After 24 h, mice were injected with LM3 mammary adenocarcinoma cells into the spleen and subjected to splenectomy. …”
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Molecular systematics: data mining of canine endogenous retroviruses, CFERV por Ekerljung, Marie

The Neospora caninum-Spain 7 isolate induces placental damage, fetal death and abortion in cattle when inoculated in early gestation por Caspe, Sergio Gaston, Moore, Prando Dadin, Leunda, Maria Rosa, Cano, Dora Beatriz, Lischinsky, Lilian Haydee, Regidor-Cerrillo, Javier, Alvarez-García, Gema, Echaide, Ignacio Eduardo, Bacigalupe, Diana, Ortega-Mora, Luis, Odeon, Anselmo Carlos, Campero, Carlos Manuel

“…For this purpose, thirteen pregnant heifers were divided into three groups as follows: group A: 7 heifers inoculated with 1 × 108 tachyzoites of Nc-Spain 7 isolate; group B: 4 heifers inoculated with 1 × 108 tachyzoites of Nc 1 strain; and group C: 2 heifers received PBS. Serum samples were collected weekly and heparinized blood samples were collected three times (0, 28 and 42 days after inoculation) by jugular venipuncture. …”
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Immune response and protection provided by live tachyzoites and native antigens from the NC-6 Argentina strain of Neospora caninum in pregnant heifers por Hecker, Yanina Paola, Moore, Prando Dadin, Quattrocchi, Valeria, Regidor-Cerrillo, Javier, Verna, Andrea Elizabeth, Leunda, Maria Rosa, Morrell, Eleonora Lidia, Ortega-Mora, Luis M., Zamorano, Patricia Ines, Venturini, María Cecilia, Campero, Carlos Manuel

“…Sixteen pregnant heifers were divided in 4 groups of 4 animals, each receiving different inoculation before mating: group A animals were intravenously (iv) inoculated with 6.25 × 107 live tachyzoites of the NC-6 strain, group B heifers were inoculated twice subcutaneously (sc) with N. caninum native antigen extract formulated with ISCOMs, group C heifers were sc injected with sterile phosphate-buffered saline (PBS) and group D heifers received sc ISCOM-matrix (ISCOMs without antigen). …”
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Contribución a la viabilidad de los sistemas de producción de carne bovina en la región subhúmeda y semiárida de la República Argentina. Estrategias de mejora por Fernandez Mayer, Anibal

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A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge por Hecker, Yanina Paola, Cóceres, Verónica Mabel, Wilkowsky, Silvina Elizabeth, Jaramillo Ortiz, Jose Manuel, Morrell, Eleonora Lidia, Verna, Andrea Elizabeth, Ganuza, Agustina, Cano, Dora Beatriz, Lischinsky, Lilian Haydee, Ángel, Sergio Oscar, Zamorano, Patricia Ines, Odeon, Anselmo Carlos, Leunda, Maria Rosa, Campero, Carlos Manuel, Morein, Bror, Moore, Prando Dadin

“…Immunogens were administered twice subcutaneously: group A animals were inoculated with three recombinant proteins (rNcSAG1, rNcHSP20, rNcGRA7) formulated with ISCOMs; group B animals received ISCOM-MATRIX (without antigen) and group C received sterile phosphate-buffered saline (PBS) only. The recombinant proteins were expressed in Escherichia coli and purified nickel resin. …”
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Development of monoclonal antibody-based assays for detection and differentiation of trypanosome species in the tsetse fly (Glossina SPP). por Bosompem, K.M.

“…This application of the technique was, however, achieved following the substitution of Na2 EDTA buffer for PBS or PSG which were used in detecting T. brucei in infected tsetse gut. …”
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Tuberculosis bovina : transmisibilidad de cepas de Mycobacterium bovis y detección de micobacterias en menudencias comercializadas en bocas de expendio de carne por Marfil, Maria Jimena

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