Resultados de búsqueda - "Muguga"

Genetic and antigenic diversity of Theileria parva in cattle in Eastern and Southern zones of Tanzania: A study to support control of East Coast fever por Elisa, M., Hasan, S.D., Moses, N., Elpidius, R., Skilton, Robert A., Gwakisa, P.S.

“…All Tp2 epitope sequences were identical to those in the T. parva Muguga strain, except for one variant of a Tp2 epitope, which is found in T. parva Kiambu 5 strain, also a component the FAO1 vaccine. …”
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Exposure of vaccinated and naive cattle to natural challenge from buffalo-derived Theileria parva por Sitt, Tatjana, Poole, Elizabeth J., Ndambuki, Gideon M., Mwaura, S., Njoroge, T., Omondi, G.P., Mutinda, M., Mathenge, J., Prettejohn, G., Morrison, W. Ivan, Toye, Philip G.

“…In this study, cattle were immunized with a live sporozoite vaccine containing three T. parva isolates (the Muguga cocktail), which has been used extensively and successfully in the field to protect against cattle-derived T. parva infection. …”
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Theileria parva live vaccination: parasite transmission, persistence and heterologous challenge in the field por Oura, C.A.L., Bishop, Richard P., Asiimwe, B.B., Spooner, P.R., Lubega, G.W., Tait, A.

“…The ‘Muguga cocktail’ live vaccine, delivered by an infection and treatment protocol, has been widely deployed in Eastern, Central and Southern Africa to protect cattle against East Coast fever, caused by Theileria parva. …”
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Immunization of cattle with a Theileria parva bovis stock from Zimbabwe protects against challenge with virulent Theileria parva parva and Theileria parva lawrencei stocks from Ken... por Irvin, A.D., Morzaria, S.P., Munatswa, F.C., Norval, R.A.I.

“…Results of an experiment conducted to determine reactions following infection of cattle with Theileria parva (Boleni) challenged with T.Parva (Muguga), T.Parva (Marikebuni), T.P., Parva (Mariakani) and T.lawrencei (Nanyuti).…”
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Cytotoxic T lymphocytes from cattle immunized against Theileria parva exhibit pronounced cross-reactivity among different strain-specific epitopes of the Tp1 antigen por Steinaa, Lucilla, Saya, R., Awino, Elias, Toye, Philip G.

“…CD8+ cells from both animals cross-reacted with the three variant CTL epitopes in interferon gamma ELISPOT assays, although the CD8+ cells from the Muguga-immunized animal showed a more epitope restricted response. …”
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The persistence of Theileria parva infection in cattle immunised using two stocks which differ in their ability to induce a carrier state: Analysis using a novel blood spot PCR ass... por Skilton, Robert A., Bishop, Richard P., Katende, J.M., Mwaura, S., Morzaria, S.P.

“…Animals infected with the Muguga stock of T. parva had parasite DNA in the peripheral blood, which could be detected by PCR, for between 33 and 129 days post-infection in different animals. …”
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Characterization of Theileria parva which infects water buck (Kobus defassa) por Stagg, D.A., Bishop, Richard P., Morzaria, S.P., Shaw, M.K., Wesonga, D., Orinda, G.O., Grootenhuis, J.G., Molyneux, D.H., Young, A.S.

“…In a separate experiment, waterbuck cells were infected and transformed in vitro by T. parva sporozoites derived from buffalo but not by cattle-derived T. parva (Muguga) sporozoites. Waterbuck cells infected in vitro with T. parva isolated from buffalo were inoculated into autologous waterbuck but no infections developed. …”
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Extensive polymorphism of Ra86 genes in field populations of Rhipicephalus appendiculatus from Kenya por Kamau, L.M., Skilton, Robert A., Githaka, Naftaly W., Kiara, Henry K., Kabiru, E., Shah, Tushaar, Musoke, A.J., Bishop, Richard P.

“…Sequencing of 19 Ra86 sequences defined two alleles differentiated by indels, encoding 693 amino acids (aa) and 654 aa respectively, from the Muguga laboratory reference strain. Ra86 sequences were also determined from gut cDNA from four field populations of R. appendiculatuscollected in different livestock production systems in Kenya. …”
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Genetic and antigenic variation of the bovine tick-borne pathogen Theileria parva in the Great Lakes region of Central Africa por Amzati, Gaston S., Djikeng, Appolinaire, Odongo, David O., Nimpaye, H., Sibeko, K.P., Muhigwa, J.-B.B., Madder, M., Kirschvink, N., Marcotty, T.

“…However, the fact that ubiquitous alleles are genetically similar to the components of the Muguga vaccine together with the limited geographical clustering may justify testing the existing trivalent vaccine for cross-immunity in the region.…”
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Rhipicephalus appendiculatus ticks transmit Theileria parva from persistently infected cattle in the absence of detectable parasitemia: Implications for East Coast fever epidemiolo... por Olds, Cassandra L., Mason, Kathleeen L., Scoles, Glen A.

“…Methods Cattle were infected with one of two stocks of T. parva (Muguga or Marikebuni). Four months post-infection when parasites were no longer detectable in peripheral blood by PCR, 500 R. appendiculatus nymphs were fed to repletion on each of the cattle. …”
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Capture-based enrichment of Theileria parva DNA enables full genome assembly of first buffalo-derived strain and reveals exceptional intra-specific genetic diversity por Palmateer, N.C., Tretina, Kyle, Orvis, J., Ifeonu, O.O., Crabtree, J., Drabék, E., Pelle, Roger, Awino, Elias, Gotia, H.T., Munro, James B., Tallon, L., Morrison, W.I., Daubenberger, C.A., Nene, Vishvanath M., Knowles, Donald P., Bishop, Richard P., Silva, Joana C.

“…We report the first buffalo-derived T. parva genome, which is ~20 kb larger than the genome from the reference, cattle-derived, Muguga strain, and contains 25 new potential genes. …”
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Alloreactive T-cell clones transformed by Theileria parva retain cytolytic activity and antigen specificity por Baldwin, Cynthia L., Teale, A.J.

“…Alloreactive T cell clones infected with T. parva (Muguga) retained expression of the T cell differentiation antigens BoT2, BoT4, BoT8 and the mature T cell antigen recognized by monoclonal antibody ILA27, as well as cytolytic function and antigen specificity, over a period of 3–4 months in continuous culture. …”
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Immunisation of cattle against East Coast fever. Experience in Zanzibar por Biwi, K.M., Rabia, R., Dolan, T.T.

“…Concern about the introduction of the T. parva strain unknown in Zanzibar by using the Muguga cocktail for immunisation against East Coast fever led the government to request ILRAD to develop a vaccine stabilate using T. parva strains found in Unguja and Pemba. …”
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The management and economics of East Coast fever por Toye, Philip G., Kiara, Henry K., Ole-MoiYoi, Onesmo, Enahoro, Dolapo K., Rich, Karl M.

“…At about the time of ILRAD's establishment in 1973, a vaccination procedure was being developed at the East African Veterinary Research Organization (EAVRO) at Muguga, Kenya. The infection-and-treatment method (ITM) is an immunization procedure against ECF. …”
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The biology of Theileria parva and control of East Coast fever—Current status and future trends por Nene, Vishvanath M., Kiara, Henry K., Lacasta, Anna, Pelle, Roger, Svitek, Nicholas, Steinaa, Lucilla

“…This has led to improved knowledge of epitope-specific T-cell responses to ITM that most likely contribute to the phenomenon of strain-specific immunity. The Muguga cocktail ITM vaccine, which provides broad-spectrum immunity to ECF is now a registered product in three countries in eastern Africa. …”
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Detection of a carrier state in Theileria parva infected cattle using the polymerase chain reaction por Bishop, Richard P., Sohanpal, B.K., Kariuki, D., Young, A.S., Nene, Vishvanath M., Baylis, H., Allsopp, B.A., Spooner, P.R., Dolan, T.T., Morzaria, S.P.

“…No amplification products were obtained from any of 7 cattle which had been infected with the T. p. parva Muguga stock. A synthetic oligonucleotide, which hybridized specifically to T. p. parva Marikebuni DNA among 6 T. parva stocks tested, was designed using sequence data from within the region of the T. parva genome amplified by the repetitive sequence primers. …”
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Challenge of Theileria parva (Boleni) immunised cattle with selected East African Theileria stocks por Hove, T., Musisi, F.L., Kanhai, G.K., Latif, A., Masaka, S., Munatswa, F.C., Pegram, R.G., Kamwendo, S.P., Quiroga, J.C., Mwangondwe, R., Dolan, T.T.

“…Theileria parva (Boleni) gave good protection against homologous challenge, the T. parva (Muguga, Kenya) and buffalo-derived T. parva (Serengeti transformed, Tanzania) parasite stocks. …”
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Systematic determination of TCR-antigen and peptide-MHC binding kinetics among field variants of a Theileria parva polymorphic CTL epitope por Svitek, Nicholas, Saya, Rosemary, Houshuang Zhang, Nene, Vishvanath M., Steinaa, Lucilla

“…The Tp967-75 CTL epitope from the Muguga strain of T. parva is polymorphic in other parasite strains. …”
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Detection of polymorphisms among Theileria parva stocks using repetitive, telomeric ribosomal DNA probes and anti-schizont monoclonal antibodies por Bishop, Richard P., Sohanpal, B.K., Spooner, P.R., Dolan, T.T., Allsopp, B.A., Morzaria, S.P.

“…Based on the comparison of repetitive DNA sequences from theT. parvaUganda and Muguga stocks, a synthetic oligonucleotide was developed which distinguished the DNA of theT. parvaUganda stock from that of 4 otherT. parvastocks on a positive/negative basis.…”
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A nested PCR assay exhibits enhanced sensitivity for detection of Theileria parva infections in bovine blood samples from carrier animals por Odongo, David O., Sunter, J.D., Kiara, Henry K., Skilton, Robert A., Bishop, Richard P.

“…The three assays were applied to samples from two calves infected with the T. parva Muguga stock. Parasite DNA was consistently detectable by the two p104 PCR assays until 48 and 82 days post-infection, respectively, and thereafter sporadically. …”
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