Search Results - "DNA"

Experimental inoculation of Neospora caninum in pregnant water buffalo by Konrad, Jose Luis, Moore, Prando Dadin, Crudeli, Gustavo Angel, Caspe, Sergio Gaston, Cano, Dora Beatriz, Leunda, Maria Rosa, Lischinsky, Lilian Haydee, Regidor-Cerrillo, Javier, Odeon, Anselmo Carlos, Ortega-Mora, Luis M., Echaide, Ignacio Eduardo, Campero, Carlos Manuel

“…Fetal fluids from cavities and tissue samples were recovered for IFAT and histopathology, immunohistochemistry and PCR, respectively. Genomic DNA from fetal tissues was used for parasite DNA detection and microsatellite genotyping in order to confirm the NC-1 specific-infection. …”
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Abortion outbreak caused by Campylobacter fetus subspecies venerealis and Neospora caninum in a bovine dairy herd by Macias Rioseco, Melissa, Caffarena, Rubén, Fraga, Martín, Silveira, Caroline, Giannitti, Federico, Canton, German Jose, Hecker, Yanina, Suanes, Alejandra, Riet Correa, Franklin

“…In these fetuses, N. caninum antigen was detected intralesionally by immunohistochemistry, and N. caninum DNA was amplified by PCR on formalin-fixed paraffin-embedded brain. …”
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Cultivo in vitro de anteras como estrategia para el mejoramiento genético de Buffelgrass (Cenchrus ciliaris L) by Carloni, Edgardo José

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Diagnosis and molecular characterization of natural cattle infections produced by Trypanosoma spp. in the Colombian Orinoco region by Cassalett B., Elizabeth Regina, V., Victor Julio, Parra A., Jorge Luis, Baldrich F., Rita Mercedes

“…Seminested PCR was performed on the 18S rDNA region. RFLP was run on the obtained products and a comparison with international standards was made. …”
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Cytogenetics of Physalis peruviana L. and Physalis floridana Rydb. genotypes with differential response to Fusarium oxysporum by Liberato Guío, Sara Alejandra, Sánchez Betancourt, Érika Patricia, Arguelles Cárdenas, Jorge, González Almario, Carolina, Núñez Zarante, Víctor Manuel

“…In P. floridana DNA content was 2.29 pg in the 2n = 24 genotype and 4.03 pg in the 2n = 48 genotype. …”
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The control of the cell cycle with particular emphasis on the G1/S transition by Andersson, Erika

“…For instance unsolved and incompletely replicated DNA in the S phase without a cell cycle stop before M phase is an area that has aroused recent interest. …”
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Application of genetic markers to forest tree species: Draft report to IPGRI of the project Developing Decision-making Strategies on priorities for conservation and use of forest g... by Namkoong, G., Koshy, M.P.

“…The application of genetic markers such as allozymes and DNA (Deoxyribose Nucleic Acid) markers has shown to be useful in studying genetic diversity in humans, animals and plants (Cruzan, 1998). …”
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Safe dissemination of germplasm resources of banana by Thomas, John, Massart, Sébastien, Houwe, Ines van den, Roux, Nicolas Stephan M., Crew, Kathy

“…This chapter focuses on key issues of the safe dissemination of banana germplasm, including germplasm conservation (field, in vitro and cryopreservation), seed and DNA banking, virus indexing and germplasm health, regulations on germplasm transfer and future trends in germplasm research.…”
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Specific PCR assay for a tannin-tolerant Selenomonas ruminantium isolate, derived from helicase coding sequences by Bishop, Richard P., Obura, M., Odongo, D., Odenyo, A.A.

“…PCR amplification by using primers from one helicase gene detected 2,000 to 5,000 EAT2 genome equivalents but did not amplify total gastrointestinal microbial DNA of nine other ungulate species.…”
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Molecular tools for characterisation of Theileria parva by Morzaria, S.P., Bishop, Richard P., Nene, Vishvanath M., Skilton, Robert A., Geysen, D., Mbogo, S.

“…The diagnostic tools are based on recombinant antigens used in ELISAs and the molecular tools are based on parasite-specific repetitive sequences, random DNAs, antigen genes, mitochondrial DNA and small subunit ribosomal RNA genes. Using mainly southern blotting technique and PCR analysis, a comprehensive characterisation of the T. parva vaccine and field stocks has been obtained. …”
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An international public partnership for genetic enhancement of cowpea using a holistic approach to biotechnology by Ortíz, R.

“…Genetic transformation and DNA marker-aided selection are two of the most important methods expected to be used in the next decade by cowpea breeders.…”
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Development and validation of a genome-wide InDel marker set discriminating the alleles between the BB-genome Oryza species and rice (O. sativa) by Malabanan-Bauan, Katrina B., Hechanova, Sherry Lou, Ahn, Eok-Keun, Li, Charng-Pei, Choi, Il-Ryong, Hernandez, Jose E., Jena, Kshirod K., Kim, Sung-Ryul

“…However, to date, there are no suitable DNA marker sets for BB-genome species (O. punctata). …”
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First report of cassava witches’ broom disease and Ceratobasidium theobromae in the Americas by Pardo, J. M, Gil-Ordoñez, A., Leiva, A. M., Enjelvin, L., Chourrot, A., Kime, S. C. K., Demade‐Pellorce, L., Marchand, M., Wilson, V., Jeandel, C., Ioos, R., Cuellar, W. J.

“…The rDNA internal transcribed spacer region was amplified using ITS4 and ITS5 primers (Landicho et al., 2024). …”
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Methodology: ssb-MASS: a single seed-based sampling strategy for marker-assisted selection in rice by Arbelaez, Juan David, Tandayu, Erwin, Reveche, Maria Ymber, Jarana, Annalhea, van Rogen, Petra, Sandager, Line, Stolt, Patrik, Ng, Enghwa, Varshney, Rajeev K., Kretzschmar, Tobias, Cobb, Joshua

“…The DNA quantity and quality extracted from single seeds from fixed lines (F6) with different physical and/or chemical properties were not significantly different. …”
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First round Dataset: Impacts of Improved Bean Technology on Smallholder Productivity and Food Security in Burundi by Katungi, Enid Mbabazi, Nduwarugira, Eric, Ntukamazina, Jean Nepomuscene, Niragira Sanctus, Mutua, Mercy Muli, Kalemera, Sylvia Monica, Nchanji, Eileen, Birachi Eliud, Rubyogo, Jean Claude

“…For variety identification, the study combined farmer recall with DNA fingerprinting. However, the initial impact analysis in 2020 was based on farmer recall, as the DNA results were not available at the time of publication. …”
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Impact of invasive weed Parthenium hysterophorus (Asteraceae) on mosquito abundance and plant-feeding behavior in an arboviral endemic region in Kenya by Osman, Tasneem, Chiuya, Tatenda, Fèvre, Eric M., Junglen, S., Borgemeister, C.

“…Mosquito plant feeding was confirmed using the anthrone test, and the ingested flora were identified via DNA barcoding of chloroplast markers, specifically matK, rbcL, and ITS2. …”
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Zoonotic surveillance for rickettsiae in domestic animals in Kenya by Mutai, B.K., Wainaina, J.M., Magiri, C.G., Ng'ang'a, J.K., Ithondeka, P.M., Njagi, O.N., Jiang, J., Richards, A.L., Waitumbi, J.N.

“…Following sequencing, 104 out of the 139 Rick17b-positive tick DNA had good reverse and forward sequences for the 3 target genes. …”
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Development and validation of allele-specific SNP/indel markers for eight yield-enhancing genes using whole-genome sequencing strategy to increase yield potential of rice, Oryza sa... by Kim, Sung-Ryul, Ramos, Joie, Ashikari, Motoyuki, Virk, Parminder S., Torres, Edgar A., Nissila, Eero, Hechanova, Sherry Lou, Mauleon, Ramil, Jena, Kshirod K.

“…DNA polymorphisms discriminating yield-positive alleles and non-target alleles for each gene were selected through sequence analysis and the allele-specific PCR-gel-based markers were developed. …”
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Analyses of mitochondrial genes reveal two sympatric but genetically divergent lineages of Rhipicephalus appendiculatus in Kenya by Kanduma, Esther G., Mwacharo, Joram M., Githaka, Naftaly W., Kinyanjui, P.W., Njuguna, J., Kamau, L.M., Kariuki, E., Mwaura, S., Skilton, Robert A., Bishop, Richard P.

“…Conclusions COI and 12S rDNA are better markers than ITS2 for studying intraspecific diversity. …”
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Molecular and pathobiological characterization of sixty one potato mop-top virus full-length cDNAs reveals great variability of the virus in the center of potato domestication, nov... by Kalyandurg, P., Gil, J.F., Lukhovitskaya, N.I., Flores, B., Muller, G., Chuquillanqui, C., Palomino, L., Monjane, A., Barker, I., Kreuze, Jan F., Savenkov, E.I.

“…To evaluate PMTV genetic variation in Peru, we generated full-length cDNA clones, which allowed reliable comparative molecular and pathobiological characterization of individual isolates. …”
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