Resultados de búsqueda - "DNA"

Studies on genetic polymorphism of improved cowpea varieties using Simple Sequence Repeats (SSR) marker por Olasan, O.J., Omoigui, L., Oluma, H.A., Aguoru, C.U., Deo, D., Ugbaa, M.S., Ezugwu, J., Ekeruo, G., Dughduh, P., Iyorkaa, N., Simon, M., Okoh, T.

“…Materials and Methods: The DNA was extracted from 14 days old seedling using the CTAB method. …”
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Haplotype-based phylogenetic analysis and population genomics uncover the origin and domestication of sweetpotato por Yan, M., Li, M., Wang, Y., Wang, X., Moeinzadeh, M., Quispe-Huamanquispe, D.G., Fan, W., Fang, Y., Nie, H., Wang, Z., Tanaka, A., Heider, B., Kreuze, Jan F., Gheysen, G., Wang, H., Vingron, M., Bock, R., Yang, J.M.

“…The tetraploid progenitor of sweetpotato was most likely I. batatas 4x, which donated the B2 subgenome, IbT-DNA1, and the lineage 2 type of chloroplast genome. …”
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A drought‐responsive rice amidohydrolase is the elusive plant guanine deaminase with the potential to modulate the epigenome por Gotarkar, Dhananjay, Longkumer, Toshisangba, Yamamoto, Naoki, Nanda, Amrit Kaur, Iglesias, Tamara, Li, Lin-Feng, Miro, Berta, Blanco Gonzalez, Elisa, Montes Bayon, Maria, Olsen, Kenneth M., Hsing, Yue-Ie Caroline, Kohli, Ajay

“…SAH is a potent inhibitor of DNA methyltransferases, the reduction of which leads to increased DNA methylation and gene silencing in Arabidopsis. …”
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Loop Mediated Isothermal Amplification for rapid detection of Ralstonia solanacearum from potato stems and tubers. Validation report por Kungu, M., Wanjiku, J., Atieno, E., Nyongesa, F., Gachamba, S., Wachira, E., Ngundo, G., Sharma, K.

“…However, the LOD was further reduced to 10 CFUs/ml and the time to positivity (Tp) reduced to 5 mins for crude DNA extracted using alkaline PEG after spiking the different CFUs into potato tuber. …”
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Diversity, recombination and misclassification in the family Geminiviridae: insight from bioinformatics analysis por Lateef, M., Nathaniel, D., Osundahunsi, B., Bretana, N.A.

“…Intergeneric pairwise identity among monopartite and Begomovirus DNA-A is ≥ 40%, while interspecies identity among Begomovirus DNA-B is ≥ 41%, corresponding to genetic variations of ≤60% and ≤59%, respectively. …”
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Differentiation, multiplication and control of bloodstream form Trypanosoma (Duttonella) vivax in mice por Mahan, S.M., Black, Samuel J.

“…Throughout the infection, variations were observed in parasite morphology, density, DNA content, number of organisms with 2 nuclei and 2 kinetoplasts and infectivity of parasites for mice. …”
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Characterization of Theileria parva which infects water buck (Kobus defassa) por Stagg, D.A., Bishop, Richard P., Morzaria, S.P., Shaw, M.K., Wesonga, D., Orinda, G.O., Grootenhuis, J.G., Molyneux, D.H., Young, A.S.

“…Species-specific synthetic oligonucleotide probes, restriction fragment length polymorphism (RFLP) analysis with cloned T. parva DNA probes, and DNA sequence analysis of the p67 sporozoite antigen gene confirmed that the waterbuck-passaged parasite was T. parva. …”
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Molecular and immunological characterisation of Theileria parva stocks which are components of the Muguga cocktail used for vaccination against East Coast fever in cattle por Bishop, Richard P., Geysen, D., Spooner, P.R., Skilton, Robert A., Nene, Vishvanath M., Dolan, T.T., Morzaria, S.P.

“…Southern blotting with four T. parva repetitive DNA probes and polymerase chain reaction (PCR)-based assays detecting polymorphism within four single copy loci encoding antigen genes. …”
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Construction and application of SSR molecular markers system for genetic diversity analysis of Chinese tartary buckwheat germplasm resources por Gao F, Zhang, Z., Wu B.

“…[Result]The optimized SSR-PCR system was as follows: 30 ng DNA template,150 μmol?L-1 dNTP,0.1 μmol?L-1 primer,2.0 U?…”
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Morphological and molecular characterisation and diagnostics of some species of Scutellonema Andrassy, 1958 (Tylenchida: Hoplolaimidae) with a molecular phylogeny of the genus por Berg, E. van den, Tiedt, L.R., Coyne, Danny L., Ploeg, A.T., Navas-Cortes, J.A., Roberts, P.A., Yeates, G.W., Subbotin, S.A.

“…Molecular analysis using the D2-D3 of 28S rRNA, ITS rRNA and the COI mtDNA gene sequences revealed two distinct genotypes within S. brachyurus samples: type A (samples from USA, Italy, Korea, Taiwan) and type B (South Africa). …”
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Two new species of Steinernema Travassos, 1927 from the humid forest of southern Cameroon por Ngo Kanga, F., Trinh, P.Q., Waeyenberge, L., Spiridonov, S.E., Hauser, S., Moens, M.

“…According to D2D3 LSU rDNA sequences the two new Steinernema species from Cameroon constitute a separate group related to inner nodes of Steinernema phylogeny.…”
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Self-excision of the antibiotic resistance gene npII using a heat inducible Cre-loxP system from transgenic potato por Cuéllar, Wilmer Jose, Gaudin, A., Solorzano, D., Casas, A., Nopo, L., Chudalayandi, P., Medrano, G., Kreuze, Jan F., Ghislain, M.

“…The footprint of the excision was shown by sequencing between T-DNA borders to correspond to a perfect recombination event. …”
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Development of field-applicable tests for rapid and sensitive detection of Candidatus Phytoplasma oryzae por Wambua, Lillian, Schneider, B., Okwaro, A., Wanga, J.O., Imali, O., Wambua, P.N., Agutu, L., Olds, Cassandra L., Jones, Christopher S., Masiga, Daniel K., Midega, C.A.O., Khana, Z., Jores, Joerg, Fischer, A.

“…Diagnosis of NSD is done by nested PCR targeting the phytoplasma DNA, which is difficult to perform in developing countries with little infrastructure. …”
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Molecular based detection, validation of a LAMP assay and phylogenetic analysis of Capripoxvirus in Kenya por Omoga, D.C.A., Macharia, M., Magiri, E., Kinyua, J., Kasiiti, J., Holton, T.

“…Aims: To optimize the different available molecular based techniques, validate the Loop-mediated isothermal amplification (LAMP) assay for detection of Capripoxviruses (CaPVs) DNA and deep sequencing to confirm the results of the LAMP assay and ensure that an effective and reliable detection method is recommended and adopted for use in Capripox diagnosis. …”
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PCR markerbased analysis of wild and cultivated yams (Dioscorea spp.)in Nigeria: genetic relationships and implication for ex situ conservation por Mignouna, H., Abang, Mathew M., Wanyera, N., Chikaleke, V., Asiedu, Robert, Thottappilly, G.

“…Twenty-five selected random decamer and two microsatellite primers were used individually and in combination to generate DNA profiles for each accession of the six Dioscorea species. …”
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Genomics of yams, a common source of food and medicine in the tropics por Mignouna, Hodeba D., Abang, Mathew M., Asiedu, Robert

“…In addition, candidate random amplified polymorphic DNA markers associated with major genes controlling resistance to Yam mosaic virus and anthracnose have been identified. …”
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Genome-wide scan reveals divergent selection among taurine and zebu cattle populations from different regions por Edea, Zewdu, Dadi, H., Dessie, Tadelle, Uzzaman, M.R., Rothschild, Max F., Kim, E.S., Sonstegard, T.S., Kim, K.S.

“…Additionally, the candidate genes were associated with functional terms (e.g. cellular response to stress, DNA repair, inflammatory response) important for physiological adaptation to environmental stresses. …”
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RdDM pathway components differentially modulate Tobamovirus symptom development por Leone, Melisa, Zavallo, Diego, Venturuzzi, Andrea Laura, Asurmendi, Sebastian

“…This study represents the initial step in exposing that DNA methylation directed by endogenous sRNAs has an important role, uncoupled to defense, in the production of symptoms associated with plant-virus interactions.…”
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Soil-borne phytopathogen and bacterial biocontrol agents using mining tools. The pathosystem phytophthora sp-Olea europea - Bacillus sp. por Rossi, Maria Sol, Casas, Roberto Raul, Michelena, Roberto, Perez, Beatriz Alida

“…Ribosomal 165 DNA was used to obtain taxonomic information about biocontrol bacteria. …”
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Estrategia de monitoreo y evaluación final : cartera programática I+D+i y documentos estratégicos del INTA por Insani, Ester Marina, Jaluf, Ana Clara

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