Search Results - "DNA"

Identification and potential use of RAPD markers linked to yam mosaic virus resistance in white yam (Dioscorea rotundata) by Mignouna, Hodeba D., Abang, Mathew M., Onasanya, A., Agindotan, B., Asiedu, Robert

“…OPW18850 and OPX15850 are the first DNA markers for YMV resistance and represent a starting point in the use of molecular markers to assist breeding for resistance to YMV.…”
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Variabilidad genética de bovinos criollos de Perú utilizando marcadores microsatélites by Aquino Villasante, Yeny Natali, Veli Rivera, Eudosio Amancio, Rivas Seoane, Emma, Rivas Palma, Victoria Esther, Estrada Zúniga, Rigoberto

“….--- [EN] We analyze a total of 326 Criollo cattle from the regions of Puno, Ayacucho and Junín in order to investigate the genetic variability of these populations. Five DNA microsatellites markers (DNA-SSR) were used (BM1818, BM1824, ETH225, ILSTS005 and ILSTS006). …”
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Early events in Agrobacterium-mediated genetic transformation of citrus explants by Pena, Leandro, Perez, Rosa, Cervera, Magdalena, Juárez, José, Navarro, Luis

“…Background and Aims Genetic transformation of plants relies on two independent but concurrent processes: integration of foreign DNA into plant cells and regeneration of whole plants from these transformed cells. …”
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The use of integrative taxonomy in determining species limits in the convergent pupa coloration pattern of Aphytis species by Pina, Tatiana, Verdú, María J., Urbaneja, Alberto, Sabater-Munoz, Beatriz

“…We propose an integrative taxonomic key for assignment to the correct species for the use in field surveys on CRS in the Iberian Peninsula which takes into account the plasticity of pupa characters and a DNA barcode key for its use with damaged or immature specimens. …”
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Identification of an Erwinia sp different from Erwinia amylovora and responsible for necrosis on pear blossoms by Roselló, Montserrat, Penyalver, Javier, Llop, Pablo, Gorris, María T., Chartier, R., Garcia, F., Monton, C., Cambra, Mariano, López, María M.

“…Polymerase chain reaction with primers from 23S rDNA sequences of E. amylovora were positive, but no signal was obtained with primers from plasmid pEa29 or from chromosomal DNA sequences of E. amylovora. …”
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Characterization and Pathogenicity of Fusicladium eriobotryae, the Fungal Pathogen Responsible for Loquat Scab by Sánchez-Torres, Paloma, Hinarejos, R., Tuset, Juan J.

“…Molecular characterization of internal transcribed spacer ribosomal DNA and, particularly, the glyceraldehyde 3-phosphate dehydrogenase gene, clearly distinguished loquat strains from Venturia inaequalis. …”
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Real-time PCR for simultaneous and quantitative detection of quarantine phytoplasmas from apple proliferation (16SrX) group by Torres, E., Bertolini, Edson, Cambra, Mariano, Monton, C., Martin, M. P.

“…An artificial template consisting in a plasmid clone of a 1785-bp DNA fragment of the 16S rRNA gene, 16S/23S rDNA spacer region, tRNA-Ile and partial 23S rRNA gene of a 'Ca. …”
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Identification and functional characterization of cation-chloride cotransporters in plants by Colmenero-Flores, José M., Martinez, German, Gamba, Gerardo, Vazquez, Norma, Iglesias, Domingo J., Brumos, Javier, Talón, Manuel

“…We have cloned an Arabidopsis thaliana cDNA encoding for a member of the cation-Cl- cotransporter (CCC) family. …”
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Assignment of SNP allelic configuration in polyploids using competitive allele-specific PCR: application to citrus triploid progeny by Cuenca, José, Aleza, Pablo, Navarro, Luis, Ollitrault, Patrick

“…Separated cluster analysis and analysis of variance (ANOVA) from mixed DNA simulating triploid and tetraploid hybrids provided 9971 correct allelic configuration. …”
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Characterization of Citrus sinensis transcription factors closely associated with the non-host response to Xanthomonas campestris pv. vesicatoria by Daurelio, Lucas D., Romero, Maria S., Petrocelli, Silvana, Merelo, Paz, Cortadi, Adriana A., Talón, Manuel, Tadeo, Francisco R., Orellano, Elena G.

“…In this study, the response of Citrus sinensis (sweet orange) leaves to Xanthomonas campestris pv. vesicatoria (Xcv), a pepper and tomato pathogenic bacterium, was analyzed through biochemical assays and cDNA microarray hybridization and compared with Asiatic citrus canker infection caused by Xanthomonas citri subsp. citri. …”
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mRNA from selected genes is useful for specific detection and quantification of viable Xanthomonas citri subsp citri by Golmohammadi, Morteza, Llop, Pablo, Scuderi, Giuseppe, Gell, I., Graham, James H., Cubero, Jaime

“…After stress treatment, only a 445-bp fragment from the gumD mRNA was detected in live Xcc cells specifically, whereas other RNA fragments, as well as DNA targets, were detected in both viable and nonviable cells. …”
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Identification of genes associated with bud dormancy release in Prunus persica by suppression subtractive hybridization by Leida, Carmen, Terol, Javier, Marti, Gracia, Agustí, Manuel, Llácer, Gerardo, Badenes, María L., Ríos, Gabino

“…Four SSH libraries were constructed, which were respectively enriched in cDNA highly expressed in dormant buds (named DR), in dormancy-released buds (RD) and in the cultivars with different chilling requirement, ‘Zincal 5’ (ZS) and ‘Springlady’ (SZ), sampled after dormancy release. …”
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High-throughput functional annotation and data mining with the Blast2GO suite by Gotz, Stefan, García-Gómez, Juan M., Terol, Javier, Williams, Tim D., Nagaraj, Shivashankar H., Nueda, María J., Robles, Montserrat, Talón, Manuel, Dopazo, Joaquín, Conesa, Ana

“…An efficient functional annotation of DNA or protein sequences is a major requirement for the successful application of these approaches as functional information on gene products is often the key to the interpretation of experimental results. …”
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Characterization of MITF gene in crayfish and their possible role in innate immunity by Wen, Yaru

“…In this study, the MITF-like protein was identified in the marbled crayfish (Procambarus virginalis). The full-length cDNA coding sequence of the most similar gene to MITF from marbled crayfish is 1284bp (427 amino acids). …”

Microbial communities in a boreal forest podzol profile and responses to the presence of Pinus sylvestris seedling roots by Jernberg, Johanna

“…The study was conducted as a microcosm experiment in combination with denaturing gradient gel electrophoresis (DGGE) fingerprinting of phylogenetic marker sequences, ITS rDNA for fungi and 16S rDNA for archaea and bacteria. …”

An estimation of thermophilic Campylobacter population in ready-to-eat roast beef and chicken sold and hygiene practices of sellers in beer bars in Arusha, Tanzania. by Toyomaki, H., Ishihara, K., Sanka, P., Kurwijila, Lusato R., Grace, Delia, Makita, K.

“…The solutions were then cultured on CCDA agar at 42°C for 48 hours and the isolates were sub-cultured on blood agar and the DNA was extracted. The extracted DNA was tested for thermophilic Campylobacter using PCR and positive DNA was tested for both C. jejuni and C. coli. …”
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Association of Burkholderia glumae and B. gladioli with panicle blight symptoms on rice in Panama by Nandakumar, R, Rush, MC, Correa Victoria, Fernando J.

“…Initial PCR analyses, made with DNA isolated directly from grain crushed in sterile water, with B. glumae specific primers (BGF 5?…”
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Identification and characterisation of differentially expressed sequence tags in trypanosoma brucei brucei: molecular cloning ,expression and analysis of ADP-ribsylation factor 1 f... by Osanya, A.O.

“…Differential hybridisation using total cDNA probes and Northern blot analyses were used to verify stage-regulation of expression of transcripts that were differentially amplified. …”
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Ancestry analysis of locally adapted crespa goats from southernmost Brazil by Lopes, D.D., Fernandez, G.P., Poli, Mario Andres, Moreira, Gilson Rudinei Pires, Freitas, T.R.O., Gonçalves, G.L.

“…In addition, a segment of the mitochondrial DNA (mtDNA) control region was sequenced to investigate the relatedness of Crespa goats to Portuguese autochthonous breeds (Algarvia, Bravia, Charnequeira, Serpentina, and Serrana). …”
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Interferon-α-modulatory sequences from the genome of porcine circovirus type 2 by Hasslung, Frida

“…Also, the inhibitory activity of ODN PCV-2/1 differed against the viral inducers Aujeszky´s disease virus (ADV) and Sendai virus (SV), and the plasmid pcDNA3. Inhibition was most effective against ADV, moderately effective against pcDNA3, but did not affect the IFN-a production induced by SV. …”
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