Resultados de búsqueda - "DNA"

Moonlighting bacteriophage proteins derepress staphylococcal pathogenicity islands por Tormo-Mas, María A., Mir, Ignacio, Shrestha, Archana, Tallent, Sandra M., Campoy, Susana, Lasa, Inigo, Barbe, Jordi, Novick, Richard P., Christie, Gail E., Penadés, José R.

“…Here we show that SaPI derepression is effected by a specific, non-essential phage protein that binds to Stl, disrupting the Stl-DNA complex and thereby initiating the excision-replication-packaging cycle of the island. …”
Enlace del recurso

A novel grapevine badnavirus is associated with the Roditis leaf discoloration disease por Maliogka, Varvara, Olmos, Antonio, Pappi, Polyxeni G., Lotos, Leonidas, Efthimiou, Konstantinos, Grammatikaki, Garyfalia, Candresse, Thierry, Katis, Nikolaos, Avgelis, Apostolos D.

“…Analysis of the NGS data revealed the presence of various known grapevine viruses and viroids as well as a hitherto uncharacterized DNA virus. The circular genome of the new virus was fully reassembled. …”
Enlace del recurso
Enlace del recurso

Analysis of Raspberry ringspot virus in raspberry by amplification of RNA-2 por Treiber, Veronika

“…RNA was extracted from raspberry stem tissue and cDNA was synthesized by reverse transcription with random primers. …”
Enlace del recurso

Biologisk framställning av insektsferomon med enzymer från japansk feberbuske por Edman, Felicia

“…I försöket genomfördes kloning med syntetiskt cDNA som slutligen transformerades in i jäst.…”
Enlace del recurso

Könsselektering av spermier från lantbrukets husdjur por Markey, Carolina

“…However, the method is associated with low fertility, high costs and uncertainty whether the sorting process contributes to DNA damage and thereby results in genetically impaired progeny. …”
Enlace del recurso

Identification of Schistosoma mansoni microRNAs por Simoes, M.C., Lee, J., Djikeng, Appolinaire, Cerqueira, G.C., Zerlotini, A., Silva Pereira, R.A. da, Dalby, A.R., LoVerde, P.T., El-Sayed, N.M., Oliveira, G.

“…In addition, we report results on the experimental detection of miRNAs by means of cDNA cloning and sequencing of size-fractionated RNA samples. …”
Enlace del recurso

Early detection of Ascochyta blight (Ascochyta rabiei) of chickpea by traditional PCR por Valetti, Lucio, Cazon, Luis Ignacio, Crociara, Clara Sonia, Pastor, Silvina Estela

“…The detection sensitivity with primers was 2 × 104 ng μl−1 genomic DNA. In inoculated plant material, PCR amplification gave a band of the expected size and no amplification was observed when DNA was from healthy and uninoculated plants. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

Occurrence and distribution of Bemisia tabaci and Trialeurodes vaporariorum (Hemiptera: Aleyrodidae) on tomato crops in Panama por Herrera-Vásquez, José A., Jaén-Sanjur, José N., Zachrisson, Bruno A., Rubio, Luis, Barba, Anovel A., Aguilera-Cogley, Vidal, Atencio, Randy, Galipienso, Luis, Mejía, Luis C.

“…DNA extracts corresponding to 57 individual whiteflies (50.9 %) rendered PCR products of expected size with B. tabaci-specific primers (37 belonged to B. tabaci MEAM1 and 20 to B. tabaci NW), whereas the remaining DNA extracts corresponding to 55 individual whiteflies (49.1 %) rendered the product of the expected size with T. vaporariorumspecific primers. …”
Enlace del recurso
Enlace del recurso

Inokulering med två hjorttryfflar på arginingödslade täckrotsplantor i Gideå plantskola por Edvardsson, Lisa

“…Five mycorrhiza species were found after DNA sequencing of the mycorrhizal root tips with different found morphotypes on seedlings from all treatments. …”
Enlace del recurso

Efecto de la adición de lactato y diacetato de sodio y almacenaje sobre las características físicas y apariencia de la carne de res para asar (M. Biceps y Triceps Brachii) por Bernal G., Guillermo A.

“…Las concentraciones utilizadas fueron 2.5/3.3% de LyDNa y dos condiciones de almacenamiento (bandejas de poliestireno, con o sin plástico PVC), en un arreglo factorial 2x2 más un testigo, con un diseño de bloques completos al azar y medidas repetidas en el tiempo. …”
Enlace del recurso

Some possible applications of molecular genetics in the conservation of wild species for crop improvement por Hodgkin, Toby, Debouck, Daniel G.

Enlace del recurso

Detection of Toxoplasma gondii infection in semen of rams used for natural mating in commercial sheep farms in Tunisia por Rouatbi, M., Amairia, S., Lahmer, M., Lassoued, N., Rekik, Mourad, Wieland, Barbara, Mwacharo, Joram M., Gharbi, M.

“…Here, to estimate the prevalence of natural infection by Toxoplasma gondii, we used PCR to amplify and detect T. gondii DNA in semen from 92 rams of three breeds from four regions in Tunisia and seroprevalence was determined using a commercial ELISA kit. …”
Enlace del recurso

First report of banana bunchy top disease caused by Banana bunchy top virus in Uganda por Ocimati, Walter, Tazuba, Anthony Fredrick Kadama, Tushemereirwe, Wilberforce K., Tugume, Joab K., Omondi, Bonaventure Aman Oduor, Acema, Dan, Were, Evans M., Onyilo, Francis, Ssekamate, M. Allan, Namanya, Priver, Kubiriba, Jerome, Erima, Rockefeller, Okurut, A. Wilson, Kutunga, David, Blomme, Guy

“…The gDNA nucleotide sequence had 98-99% similarity with BBTV isolates form different countries in Africa, Asia, South Pacific and the USA. …”
Enlace del recurso

An inexpensive CRISPR-based point-of-care test for the identification of meat species and meat products por Tao, D., Xiao, X., Lan, X., Xu, B., Wang, Y., Khazalwa, Emmanuel M., Pan, W., Ruan, J., Jiang, Y., Liu, X., Li, C., Ye, R., Li, X., Xu, J., Zhao, S., Xie, S.

“…The test turnaround time from DNA extraction to visualization was approximately 40 min. …”
Enlace del recurso

Homing in on endogenous badnaviral elements: Development of multiplex PCR-DGGE for detection and rapid identification of badnavirus sequences in yam germplasm por Silva, G., Bömer, M., Turaki, A.A., Nkere, C., Kumar, P. Lava, Seal, S.

“…Viruses of the genus Badnavirus (family Caulimoviridae) are double-stranded DNA-reverse transcribing (dsDNA-RT) plant viruses and have emerged as serious pathogens of tropical and temperate crops globally. …”
Enlace del recurso

Optical mapping compendium of structural variants across global cattle breeds por Talenti, A., Powell, J., Wragg, D., Chepkwony, Maurine, Fisch, A., Ferreira, B.R., Mercadante, Maria E.Z., Santos, I.M., Ezeasor, C.K., Obishakin, E.T., Muhanguzi, D., Amanyire, W., Silwamba, I., Muma, J.B., Mainda, G., Kelly, R.F., Toye, Philip G., Connelley, T., Prendergast, J.

“…Optical mapping is an alternative approach for mapping SVs that has been shown to have higher sensitivity than DNA sequencing approaches. The aim of this project was to use optical mapping to develop a high-quality database of structural variation across cattle breeds from different geographical regions, to enable further study of SVs in cattle. …”
Enlace del recurso

Monitoring of host suitability and defense-related genes in wheat to Bipolaris sorokiniana por Alkan, Mehtap, Bayraktar, Harun, Imren, Mustafa, Ozdemir, Fatih, Lahlali, Rachid, Mokrini, Fouad, Paulitz, Timothy C., Dababat, Abdelfattah A., Özer, Göksel

“…Three primer sets, BsGAPDHF/BsGAPDHR, BsITSF/BsITSR, and BsSSUF/BsSSUR, were designed based on glyceraldehyde-3-phosphate dehydrogenase, internal transcribed spacers, and 18S rRNA loci of B. sorokiniana with detection limits of 1, 0.1, and 0.1 pg of pathogen DNA, respectively. The qPCR assay was highly sensitive and did not amplify DNA from the other closely related fungal species and host plants. …”
Enlace del recurso

Resistance response of drought and heat tolerant spring wheat lines against the cereal cyst nematode, heterodera filipjevi por Atiya, Osameh, Dababat, Abdelfattah A., Erginbas-Orakci, Gul, Singh, Sukhwinder, Toktay, Halil

“…Three primer sets, BsGAPDHF/BsGAPDHR, BsITSF/BsITSR, and BsSSUF/BsSSUR, were designed based on glyceraldehyde-3-phosphate dehydrogenase, internal transcribed spacers, and 18S rRNA loci of B. sorokiniana with detection limits of 1, 0.1, and 0.1 pg of pathogen DNA, respectively. The qPCR assay was highly sensitive and did not amplify DNA from the other closely related fungal species and host plants. …”
Enlace del recurso

Comparative analysis of 343 plastid genomes of Solanum section Petota: Insights into potato diversity, phylogeny, and species discrimination por Yan, L., Zhu, Z., Wang, P., Fu, C.D., Guan, X., Kear, P., Zhang, C.C., Zhu, G.Y.

“…Overall, our study provided not only new insights into phylogeny and DNA barcoding of potato but also provided valuable genetic data resources for further systematical research of Petota.…”
Enlace del recurso

Resolving the evolution of extant and extinct ruminants with high-throughput phylogenomics por Decker, J., Pires, J.C., Conant, G.C., McKay, S.D., Heaton, M.P., Chen, K., Cooper, A., Vilkki, J., Seabury, C.M., Caetano, A.R., Johnson, G.S., Brenneman, R.A., Hanotte, Olivier H.

“…Here we demonstrate that a genome-wide single nucleotide polymorphism (SNP) genotyping platform designed for one species can be used to genotype ancient DNA from an extinct species and DNA from species diverged up to 29 million years ago and that the produced genotypes can be used to resolve the phylogeny for this rapidly radiated infraorder. …”
Enlace del recurso