Search Results - "DNA"

Isolation of high-quality total RNA from leaves of myrciaria dubia Camu camu by Castro Gómez, Juan Carlos, Egoavil Reátegui, Alina del Carmen, Torres Flores, Julián, Ramírez Saavedra, Roberson, Imán Correa, Sixto Alfredo

“…Furthermore, electrophoretic analysis showed the integrity of isolated RNA and the absence of DNA. Another proof of the high quality of our purified RNA was the successful cDNA synthesis and amplification of a segment of the M. dubia actin 1 gene. …”
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Theileria cervi in Amblyomma neumanni (Acari: Ixodida, Ixodidae) collected on horses from North-western Argentina by Montini, Martina, Torrents, Jorgelina, Nava, Santiago, Sebastian, Patrick

“…A total of 97 adult ticks from four different provinces (Jujuy, Santiago del Estero, Salta, Tucumán) were included in this study. Genomic DNA was isolated, and conventional PCR assays were applied for the detection of the 18S rDNA gene of Babesia and Theileria species. …”
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Immunization of lambs with the S48 strain of Toxoplasma gondii reduces tissue cyst burden following oral challenge with a complete strain of the parasite by Katzer, Frank, Canton, German Jose, Burrels, Alison, Palarea-Albaladejo, Javier, Horton, Ben, Bartley, Paul M., Pang, Yvonne, Chianini, Francesca, Innes, Elisabeth A., Benavides-Silván, Julio

“…S48 T. gondii DNA was found in muscle or lymph nodes until 42 days post infection, suggesting that parasite DNA or tachyzoites could persist longer after immunization than previously thought. …”
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Development of a Highly Sensitive Nested-PCR Procedure Using a Single Closed Tube for Detection of Erwinia amylovora in Asymptomatic Plant Material by Llop, Pablo, Bonaterra, Anna, Penyalver, Javier, López, María M.

“…The presence of inhibitors in plant material, very common in E. amylovora hosts, is overcome with this system in combination with a simple DNA extraction protocol because it eliminates many of the inhibitory compounds. …”
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Development of a loop-mediated isothermal amplification (LAMP) and a direct LAMP for the specific detection of Nosema ceranae, a parasite of honey bees by Lannutti, Lucas, Mira, Anabela, Basualdo, Marina, Rodriguez, Graciela Adriana, Erler, Silvio, Silva, Victoria Ayelén, Gisder, Sebastian, Genersch, Elke, Florin-Christensen, Mónica, Schnittger, Leonhard

“…The use of a rapid, sensitive, and inexpensive DNA-based molecular detection method assists in the surveillance and eventual control of this pathogen. …”
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Development of a non-radioactive molecular hybridization probe for detecting Strawberry mottle virus in strawberry by Asinari, Florencia, Cafrune, Eva Encarnacion, Guzman, Fabiana, Conci, Luis Rogelio, Conci, Vilma Cecilia

“…In this study, a non-radioactive molecular probe was developed for SMoV detection. The cDNA was synthesized by RT-PCR using specific primers designed from the 3’UTR region of the viral genome. …”
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Effect of the addition of phytosterols and tocopherols on Streptococcus thermophilus robustness during industrial manufacture and ripening of a functional cheese as evaluated by qP... by Pega, Juan Franco, Rizzo, Sergio Anibal, Perez, Carolina Daiana, Rossetti, Luciana, Diaz, Gabriela, Ruzal, S.M., Nanni, Mariana, Descalzo, Adriana Maria

“…We also showed for the first time that the addition of phytosterols at functional concentrations, not only did not affect starter performance but also correlated with a significant increase in target DNA and cDNA levels in most of the time points evaluated throughout cheesemaking. …”
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Caracterización taxonómica y análisis de la variabilidad del agente causal del cancro del tallo de la soja en Buenos Aires (2005/2007) by Grijalba, Pablo Enrique, Ridao, Azucena del Carmen, Guillin, Eduardo Alejandro

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Nivel de ADN-Ploidía en poblaciones sudamericanas y norteamericanas de la gramínea nativa disyunta Trichloris crinita (Chloridoideae, Poaceae) by Carloni, Edgardo José, Quiroga, Raul Emiliano, Grunberg, Karina, Premoli, Andrea Cecilia

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Nodulär onchocercos på kronhjort (Cervus elaphus) i Sverige by Boijsen, Bim

“…Artbestämning av parasiter från 14 slumpvist utvalda noduliprover från lika många hjortindivider från sju olika hägn gjordes genom sekvensering av 12S- och 16S generna samt ND5 regionen i parasitens mitokondriella DNA. Samtliga DNA-sekvenser var identiska med tidigare publicerade sekvenser av Onchocerca flexuosa. …”
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Genetisk variation hos svenska lantrashöns by Englund, Thomas

“…Skånsk blommehöna är den ras som varierar mest med fyra olika haplotyper av mtDNA. Kindahöna och Åsbohöna har två olika haplotyper vardera och Ölandshöna skiljer sig mest med en egen haplotyp som finns hos alla tre provtagna individer. …”
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Los desafíos están en el ADN mismo de la EMBRAPA by Tallarico, F., Germano, R.

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Morphology, Phylogeny and Culture Characteristics of Ganoderma gibbosum Collected from Kunming, Yunnan Province, China by Luangharn, T., Karunarathna, Samantha C, Mortimer, P.E., Hyde, Kevin D., Xu, J.C.

“…The specimens are described and illustrated based on macro- and micro-morphological characteristics. Total DNA of the eight G. gibbosum strains were extracted using the Biospin Fungal Extraction Kit following manufacturer protocol. …”
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Origin and genetic diversity of Nepalese indigenous goats (Capra hircus) by Gorkhali, N.A., Lin, J., Sapkota, Suraj, Pokhrel, B.R., Ma, Y.H., Han Jianlin

“…The complex mtDNA diversity and structure identified among indigenous Nepalese goats can be explained by gene flow through ancient trading and the current ‘free’ movement of goats across the geographic vicinities in India and China. …”
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Tissue-specific enhancement of OsRNS1 with root-preferred expression is required for the increase of crop yield by Gho, Yun-Shil, Choi, Heebak, Moon, Sunok, Kim, Sung-Ryul, Ha, Sun-Hwa, Jung, Ki-Hong

“…OsRNS1 was found to be involved in root development through T-DNA insertional mutant analysis and gene editing mutant analysis. …”
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Molecular markers and their use in marker-assisted selection in rice by Jena, K.K., Mackill, D.J.

“…The use of cost‐effective DNA markers derived from the fine mapped position of the genes for important agronomic traits and MAS strategies will provide opportunities for breeders to develop high‐yielding, stress‐resistant, and better‐quality rice cultivars.…”
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Investigating the diversity of plant-parasitic nematodes associated with coffee in Kenya, using morphological and molecular approaches by Maosa, J., Githinji, Z., Gitonga, D., Nguyen, H.T., Couvreur, M., Cortada, L., Coyne, D.L., Bert, W.

“…For T. obscurus, the 18S rDNA sequences obtained in this study are the first ever reported for the genus. …”
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Differential transcription of two highly divergent gut-expressed Bm86 antigen gene homologues in the tick Rhipicephalus appendiculatus (Acari: Ixodida) by Kamau, L., Skilton, Robert A., Odongo, David O., Mwaura, S., Githaka, Naftaly W., Kanduma, Esther G., Obura, M., Kabiru, E., Orago, A., Musoke, A.J., Bishop, Richard P.

“…Reverse-transcription PCR of gut cDNA showed transcription of only one genotype in individual female ticks. …”
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Characterization of a small variable surface glycoprotein from Trypanosoma vivax by Gardiner, Peter R., Nene, Vishvanath M., Barry, M.M., Thatthi, R., Burleigh, B., Clarke, M.W.

“…The molecular weight of the VSG predicted from the translated cDNA sequence is similar to that of the native molecule and in support of the biochemical data it is devoid of sites for N-linked glycosylation. …”
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Infection of bovine T cell clones with genotypically distinct Theileria parva parasites and analysis of their cell surface phenotype by Conrad, P.A., Baldwin, Cynthia L., Brown, W.C., Sohanpal, B.K., Dolan, T.T., Goddeeris, Bruno M., Demartini, J.C., Ole-MoiYoi, O.K.

“…The parasites were characterizedin vitroby hybridization ofT. parva- specific DNA probes to Southern blots of endonuclease-digested DNA from the infected T cell clones. …”
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