Search Results - "DNA"

A next generation sequencing approach to constructing a genetic map for Populus tremula by Mujtaba, Tahir

“…RRL construction involves the use of restriction enzyme digestion of genomic DNA to generate a consistent set of DNA fragment from different samples and serves the purpose of efficiently reducing genomic representation and subsequent volume of sequence data that is required for polymorphism identification. …”

Aislamiento y caracterización de marcadores moleculares microsatélites a partir de la construcción de librerías genómicas enriquecidas de camote. ( Ipomoea batatas (L.) Lam.) by Yañez, V.

“…Ser una técnica basada en el PCR, necesitar pequeñas cantidades de DNA y su naturaleza codominante, han hecho de ellos una herramienta muy popular en trabajos de investigación en animales y plantas. …”
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Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America by San Blas, Germán, Baudino, Estela Maris, Silva Dias, Fernando Maia, Dolibaina, Diego Rodrigo, Specht, Alexandre, Martins Casagrande, Mirna, Cornejo, Paula, Guillot Giraudo, Walter, Hendrik Mielke, Olaf Hermann

“…These results will allow identifying these species using DNA ‘barcodes’, either for pest management or general biological studies. …”
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Optimization of immunoassay parameters in multiplex in the high throughput protein detection technique Proximity Extension Assay by Källman, Christine

“…Two proximity probes containing oligonucleotide sequences bind pairwise to target protein and when brought in proximity, a DNA polymerase will extend a hybridization arm from one probe over to the second forming a double-stranded DNA sequence that can serve as a template in real-time PCR. …”
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Determinación del umbral de detección de Pseudococcus viburni (Hemiptera: Pseudococcidae) por PCR by Vera Macaya, Diana Lorena, Garrido, Silvina Alejandra, Aun, Elizabeth De Los Angeles, Lago, Jonatan, Cichon, Liliana

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Fingerprinting plant killers by Cooke, David

“…David Cooke explains how DNA-based molecular diagnostics is helping researchers to fight plant diseases caused by a notorious family of fungi.…”
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Reproductive, pathogenic and genotypic characterisation of five Meloidogyne graminicola populations from the Philippines on susceptible and resistant rice varieties by Cabasan, Ma. Teodora Nadong, Kumar, Arvind, Bellafiore, Stéphane, De Waele, Dirk

“…A search onM. graminicolapopulations from the Philippines for single-nucleotide polymorphism on the sequences of Internal Transcribed Spacer (ITS) of rDNA genes and mtDNA indicated only few points of heteroplasmy. …”
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Cowdria ruminantium infection in ticks in the Kruger National Park by Peter, T.F., Bryson, N.R., Perry, Brian D., O'Callaghan, C.J., Medley, G.F., Smith, G.E., Mlambo, G., Horak, I.G., Burridge, M.J., Mahan, S.M.

“…The remaning infections were below the detection limit of the DNA probe, which is approximately 70,000 organisms. …”
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Detection of Mycobacterium bovis lymphocyte stimulating antigens in culture filtrates of a recombinant Mycobacterium smegmatis cosmid library by Gormley, E., Fray, L., Sandall, L., Ke, G., Dupont, C., Carpenter, E.

“…Analysis of the cosmid DNA associated with these positive CF demonstrated that several contained homologous DNA sequences. …”
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Burkholderia mimosarum sp. nov., isolated from root nodules of Mimosa spp. from Taiwan and South America by Chen, W.M., James, EK, Coenye, T., Chou, JH, Barrios, E., Faria, S.M. de, Elliott, G.N., Sheu, SY, Sprent, JI, Vandamme, P

“…A polyphasic approach, including DNA–DNA reassociation, whole-cell protein analysis, fatty acid methyl ester analysis and extensive biochemical characterization, was used to clarify the taxonomic position of these strains: all 14 strains were classified as representing a novel species, for which the name Burkholderia mimosarum sp. nov. is proposed. …”
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Differential gene expression in East African highland bananas (Musa spp.): interactions between nonpathogenic Fusarium V5w2 and Radopholus similis by Paparu, P., Dubois, T., Coyne, Danny L., Viljoen, Altus

“…Using complementary (c)DNA-amplified fragment length polymorphism (cDNA-AFLP), we identified genes induced in nematode-susceptible and -tolerant bananas following colonization by non-pathogenic endophytic F. oxysporum isolate V5w2. …”
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Censo Nacional de Bibliotecas Gubernamentales. Presentación de resultados by Streletzxy, Graciela, Rosenberg, Alberto, Bubien, Marcela, Bonomo, Adriana Julia, Baez Becker, Natalia, dos Santos, Silvina, Paroli, Ciro

“…DNA SITyP…”
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Development of Indel Markers from Citrus Clementina (Rutaceae) Bac-End Sequences and Interspecific Transferability in Citrus by Ollitrault, Frederique, Terol, Javier, Martin, Alena Alonso, Pina, José A., Navarro, Luis, Talón, Manuel, Ollitrault, Patrick

“…Methods and Results: Using polyacrylamide gel electrophoresis and DNA silver staining, 89 indel markers were tested for their transferability and polymorphism. …”
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Structure-function analysis of the SaPIbov1 replication origin in Staphylococcus aureus by Ubeda, Carles, Tormo-Mas, María A., Penadés, José R., Novick, Richard P.

“…Since SaPI replication can be initiated on linear DNA, it is suggested that bilateral binding may be necessary to constrain the intervening DNA to enable Rep-driven melting. …”
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Molecular evidence confirms occurrence of Rhipicephalus microplus Clade A in Kenya and sub-Saharan Africa by Kanduma, Esther G., Emery, D., Githaka, Naftaly W., Nguu, E.K., Bishop, Richard P., Šlapeta, J.

“…More recently, the pathogenic Babesia bovis has been detected in cattle blood DNA. The status of R. microplus in Kenya remains unknown. …”
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Shining a Brighter Light: Comprehensive Evidence on Adoption and Diffusion of CGIAR-related Innovations in Ethiopia by Kosmowski, Frederic, Alemu, Solomon, Mallia, Paola, Stevenson, James R., Macours, Karen

“…The 2019 round included, for the first time, DNA fingerprinting of crop varieties, alongside detailed protocols for livestock and natural resource management. …”
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High-Density Lipoprotein Cholesterol Concentrations and Particle Size Are Modified by Dietary Zinc in Men by Shenvi, Swapna, Chen, Kevin, Kim, Elijah, Zyba, Sarah, King, Sarah, Shigenaga, Mark, Killilea, David, Holland, Tai, Sutherland, Barbara, Krauss, Ronald, King, Janet C.

“…Based on our previous observation of higher DNA strand breaks in the low zinc metabolic period and literature reports linking cancer to low HDL-c levels, we performed a correlation analysis of DNA strand breaks and HDL subspecies. …”
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Removal of transgenes and Evaluation of yield penalties in genome edited bacterial blight resistant rice varieties by Loo, Eliza P.I., Huguet‐Tapia, José C., Selvaraj, Michael, Stiebner, Melissa, Killing, Britta, Buchholzer, Marcel, Schepler‐Luu, Van, Hartwig, Thomas, Valdéz Gutierrez, Sandra P., Rast‐Somssich, Madlen I., Balahadia, Christian Paolo, Slamet‐Loedin, Inez H., Oliva, Ricardo, Szurek, Boris, Tohme, Joe, Chavarriaga, Paul, White, Frank F., Yang, Bing, Frommer, Wolf B.

“…Various strategies were evaluated, including herbicide tolerance, PCR, DNA gel blotting, whole genome sequencing (WGS), and specific tests stipulated by country‐specific biosafety guidelines. …”
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Molecular biodiversity of cassava begomoviruses in Tanzania: evolution of cassava geminiviruses in Africa and evidence for East Africa being a center of diversity of cassava gemini... by Ndunguru, J., Legg, James P., Aveling, T., Thompson, G., Fauquet, Claude M.

“…The complete nucleotide sequence of EACMCV-[TZ1] DNA-A and DNA-B components shared a high overall sequence identity to EACMCV-[CM] components (92% and 84%). …”
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First report of cassava mosaic disease and cassava mosaic geminiviruses in Gabon by Legg, James P., Ndjelassili, F., Okao-Okuja, G.

“…Virus diagnoses were subsequently made from DNA samples using both specific primer PCR (Zhou et al., 1997) and RFLP analysis involving restriction digestion by EcoRV and MluI of near full-length DNA-A fragments amplified using abutting primer PCR with universal geminivirus primers (Briddon & Markham, 1994). …”
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