Search Results - "DNA"

  1. Stability of cassava plants at the DNA level after retrieval from 10 years of in vitro storage by Angel, F., Barney, Victoria E., Tohme, Joseph M., Roca, W.

    Published 1996
    “…RFLPs with homologous probes, RAPDs with twenty primers and DNA fingerprinting with M13 probe were tested to detect variation at DNA level in cassava plants after ten-years in vitro storage. …”
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  2. Field collection,preservation and large scale DNA extraction procedures for cassava (Manihot esculenta Crantz.) by Bhattacharjee, Ranjana, Ferguson, Morag E., Gedil, Melaku A, Dumet, D., Ingelbrecht, I.L.

    Published 2009
    “…Some genetic studies using molecular methods such as diversity assessment or marker-assisted selection require collection of a large number of samples from fields located in the vicinity or in remote areas, followed by isolation of good quality DNA in a short time span. In the present study, different tissue preservation methods were compared for subsequent DNA extraction using a modified CTAB method in two 96-well plates, following grinding of leaf tissues with a GenoGrinder 2000. …”
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    Journal Article
  3. Molecular characterization of the 5S rDNA non-transcribed spacer and reconstruction of phylogenetic relationships in Capsicum by Grabiele, Mauro, Aguilera, Patricia Mabel, Ducasse, Daniel Adrian, Debat, Humberto Julio

    Published 2022
    “…In this original report we PCR amplified, cloned, sequenced and characterized the 5S rDNA non-transcribed spacer -NTS- in 23 taxa of nine clades of Capsicum, divergent at geographical origin and fruit and chromosome traits, and compared the NTS features throughout Solanaceae. …”
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  4. The effects of Quaternary glaciations in Patagonia as evidenced by chloroplast DNA phylogeography of Southern beech Nothofagus obliqua by Azpilicueta, Maria Marta, Marchelli, Paula, Gallo, Leonardo Ariel

    Published 2023
    “…To evaluate the impact of these range movements on the genetic structure of populations, we performed a phylogeographical analysis of the species Nothofagus obliqua with chloroplast DNA markers. A total of 27 populations covering the whole natural distribution range were analyzed using polymerase chain reaction-restriction fragment length polymorphism. …”
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  5. Optimization and comparative analysis of LAMP and PCR techniques for the detection of leptospiral DNA in Golden Syrian hamsters by Hamer, Micaela, Watanabe, Olivia, Saraullo, Vanina Rosa, Ortega, Facundo Miguel, Sanchez, Maria Cristina, Martinez, Mara Leila, Brihuega, Bibiana Felicitas, Grune Loffler, Sylvia

    Published 2023
    “…LAMP assay combined with calcein showed a tenfold higher limit of detection (1 ng of leptospiral DNA per reaction) than LAMP combined with hydroxynaphthol blue or end-point PCR lipL32 (10 ng of DNA per reaction). …”
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  6. Genetic variation and heteroplasmy of Varroa destructor inferred from ND4 mtDNA sequences by Muntaabski, Irina, Russo, Romina Maria, Liendo, María Clara, Palacio, María Alejandra, Cladera, Jorge Luis, Lanzavecchia, Silvia Beatriz, Scannapieco, Alejandra Carla

    Published 2020
    “…Phoretic mites were collected from managed A. mellifera colonies in ten localities, and four mitochondrial DNA (mtDNA) regions (COXI, ND4, ND4L, and ND5) were analyzed. …”
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  7. Differential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species by Cascales, Jimena, Acevedo, Raúl Maximiliano, Paiva, Daniela Ivana, Gottlieb, Alexandra Marina

    Published 2021
    “…Differential epigenetic (DNA cytosine methylation) and gene expression patterns were investigated in reproductive and vegetative organs from Ilex paraguariensis and I. dumosa, at distinct developmental stages. …”
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