Resultados de búsqueda - "Copi"

Comprehensive analysis of equid herpesvirus recombination : an insight into the repeat regions por Tau, Rocio Lucia, Ferreccio, Carola Maria, Bachir, Natalia Veronica, Torales, Fatima Anabel, Romera, Sonia Alejandra, Maidana, Silvina Soledad

“…This work aimed to analyze interspecific recombination between all equid alphaherpesvirus species, using genomes of EHV-1, EHV-3, EHV-4, EHV-6, EHV-8, and EHV-9 available in GenBank. 14 events of recombination by RDP4 and Simplot between EHV-1 x EHV-4, EHV-1 x EHV-9, EHV-8 x EHV-1, and EHV-8 x EHV-9 were identified. 10 out of 14 events involved ORF64, a double-copy gene located at the repeat regions that codifies for the infected cell protein 4 (ICP4). …”
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Comparison of real-time PCR and nested PCR based on the HlyA gene for the detection of Listeria monocytogenes. Application on cheese samples por Zbrun, María Virginia, Moreno, Nadia, Camussone, Cecilia, Signorini Porchiett, Marcelo Lisandro, Primo, María Evangelina

“…Both molecular techniques showed the same sensitivity, 30 copies/reaction or 3.5 UFC/25 g, when plasmid DNA standard or artificially contaminated cheese samples were used. …”
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Inter-laboratory comparison of eleven quantitative or digital PCR assays for detection of proviral bovine leukemia virus in blood samples por Pluta, Aneta, Jaworski, Juan Pablo, Droscha, Casey, VanderWeele, Sophie, Taxis, Tasia M., Valas, Stephen, Brnić, Dragan, Jungić, Andreja, Ruano, María José, Sánchez, Azucena, Murakami, Kenji, Nakamura, Kurumi, Puentes, Rodrigo, De Brun, María L., Ruiz, Vanesa, Ladera Gómez, Marla Eliana, Lendez, Pamela Anahí, Dolcini, Guillermina Laura, Fernandes Camargos, Marcelo, Fonseca, Antonio, Barua, Subarna, Wang, Chengming, Giza, Aleksandra, Kuźmak, Jacek

“…Agreement amongst different assays was linked to BLV copy numbers present in samples and the characteristics of each assay (i.e., BLV target sequence). …”
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Effect of High-Pressure Processing on Quality and Microbiological Properties of a Fermented Beverage Manufactured from Sweet Whey Throughout Refrigerated Storage por Pega, Juan Franco, Denoya, Gabriela Inés, Castells, M.L., Sarquis, S., Aranibar, G.F., Vaudagna, Sergio Ramon, Nanni, Mariana

“…Conversely, quantitative PCR (qPCR) and reverse transcription-qPCR (RT-qPCR) revealed that bacterial DNA or mRNA levels persisted after HPP (> 1.4 × 105 genome or cDNA copies/mL), even upon 400-MPa treatments. As a whole, this study indicated that HPP preserved the quality of the beverage until 45 days post-HPP, which is longer than the shelf-life of conventional fermented beverages obtained from milk. …”
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Five-Year Molecular Surveillance Reveals Widespread Foodborne Enteric Viruses in Bivalve Mollusks From Golfo Nuevo, Argentina por Frydman, Camila Ayelén, Miño, Samuel, Barbieri, Elena Susana, Galeano, Fabiana Solange, Vaudagna, Sergio Ramon, Parreño, Gladys Viviana, Mozgovoj, Marina Valeria

“…Mean viral loads ranged from 4.8 to 6.3 log10 genomic copies/g of bivalve mollusks. Temporal trends revealed a significant decline in detection post-pandemic for RVA, NoV GII, and HAV, while AdV and HEV rates remained stable. …”
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Dynamics of perinatal bovine leukemia virus infection por Gutierrez, Gerónimo, Alvarez, Irene, Merlini, Ramiro, Rondelli, Flavia, Trono, Karina Gabriela

“…High PVLs persisted until the end of the study, and, in two animals, exceeded one BLV copy per cell. Two other calves maintained a high PVL from birth until the end of the study. …”
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MITE Tracker : an accurate approach to identify miniature inverted-repeat transposable elements in large genomes por Crescente, Juan Manuel, Zavallo, Diego, Helguera, Marcelo, Vanzetti, Leonardo Sebastian

“…Background: Miniature inverted-repeat transposable elements (MITEs) are short, non-autonomous class II transposable elements present in a high number of conserved copies in eukaryote genomes. An accurate identification of these elements can help to shed light on the mechanisms controlling genome evolution and gene regulation. …”
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Novel biotechnological platform based on baculovirus occlusion bodies carrying Babesia bovis small antigenic peptides for the design of a diagnostic enzyme-linked immunosorbent ass... por Lopez, Maria Gabriela, Pallares, H.M., Alfonso, Victoria, Carmona, S. J., Farber, Marisa Diana, Taboga, Oscar Alberto, Wilkowsky, Silvina Elizabeth

“…An Sf9POLHE44G packaging cell line infected with recombinant baculoviruses carrying POLH-ApBb fusions yielded higher levels of chimeric polyhedra, highlighting the advantage of a trans-contribution of a mutant copy of polyhedrin. Finally, the use of dissolved recombinant polyhedra as antigens was successful in an ELISA assay, as B. bovis-positive sera recognized the fusion POLH-ApBb. …”
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Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus por Tignon, M., Gallardo, C., Iscaro, C., Hutet, E., Stede, Y. van der, Kolvasov, D., Mia, G.M. de, Potier, M.F. le, Bishop, Richard P., Arias, Marisa, Koenen, F.

“…The limit of detection of the new real-time PCR assay is 5.7 to 57copies of the ASFV genome. High accuracy, reproducibility and robustness of the PCR assay (CV ranging from 0.7 to 5.4%) were demonstrated both within and between laboratories using different real-time PCR equipments. …”
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Genomic resources to guide improvement of the shea tree por Hale, I., Ma, X., Melo, A.T.O., Padi, Francis K, Hendre, P.S., Kingan, S.B., Sullivan, S.T., Chen, S., Boffa, J.-M., Muchugi, Alice, Danquah, A., Barnor, M.T., Jamnadass, Ramni H., Peer, Y. van de, Deynze, A. van

“…In a first genome-wide look at the suite of fatty acid (FA) biosynthesis genes that likely govern stearin content, the primary determinant of shea butter quality, relatively high copy numbers of six key enzymes were found (KASI, KASIII, FATB, FAD2, FAD3, and FAX2), some likely originating in shea’s more recent WGD event. …”
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HOMEOBOX2, the paralog of SIX-ROWED SPIKE1/HOMEOBOX1, is dispensable for barley spikelet development por Thirulogachandar, Venkatasubbu, Govind, Geetha, Hensel, Götz, Kale, Sandip M., Kuhlmann, Markus, Eschen-Lippold, Lennart, Rutten, Twan, Koppolu, Ravi, Rajaraman, Jeyaraman, Palakolanu, Sudhakar Reddy, Seiler, Christiane, Sakuma, Shun, Jayakodi, Murukarthick, Lee, Justin, Kumlehn, Jochen, Komatsuda, Takao, Schnurbusch, Thorsten, Sreenivasulu, Nese

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Fruit commodity development in Goma District through farmer-based improved fruit seedling supply system: Experiences from IPMS por Baredo, Y., Berhe, Kahsay, Hoekstra, Dirk

“…The 9 model farmers have now raised 17,000 avocado and mango root stock seedling at their own cost amounting to Birr 85,000 which will be ready for grafting starting from January 2010. In addition 3 copy farmers in Kilole PA are also engaged in improved fruits seedlings production. …”
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Telomeric features of Theileria parva mitochondrial DNA derived from cycle sequence data of total genomic DNA por Shukla, G.C., Nene, Vishvanath M.

“…Rather than using purified mtDNA as template, we reasoned that since the T. parva genome is only about 10 (7) bp (4,5), and parasite mtDNA is present in multiple copies (1), we should be able to employ cycle sequence analysis of total genomic DNA to obtain mtDNA sequences. …”
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Replacing two conserved amino acids, (Asn-52 to Val and Try-67 to phe), in the Iso-1-cytochrome c gene has little effect on the function of the protein. por McOdimba, F.A.

“…The two amino acids (Tyr-67 and Asn-52) were replaced with Phe and Val on the wild type iso-I-cytochrome c gene in a multi-copy pING4 expression plasmid vector which already had a Cys-l 02 to Thr mutation. …”
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Manual for cocoa extension in Ghana por Ghana Cocoa Board

“…Manual Draft Copy: For sustainable production of cocoa it is the view of the Cocoa Health and Extension Division (CHED) of the COCOBOD to have highly trained and motivated human resource to facilitate the processes, which can lead to increased and sustained cocoa production. …”
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Decision-making tool for national implementation of the Plant Treaty's multilateral system of access and benefit-sharing por Joint Capacity Building Programme

“…The publication is available in Spanish, French and Arabic: SP: https://hdl.handle.net/10568/97878 FR: https://hdl.handle.net/10568/98234 AR: https://hdl.handle.net/10568/101420 Interested users can request hard copies of the tool to Bioversity International by sending and email to Evelyn Clancy: e.clancy@cgiar.org…”
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The chlorotic symptom induced by Sunflower chlorotic mottle virus is associated with changes in redox-related gene expression and metabolites por Rodriguez, Marianela, Muñoz, Nacira Belen, Lenardon, Sergio Luis, Lascano, Hernan Ramiro

“…A positive correlation between chlorotic symptom and number of viral copies was also observed. Changes in hydrogen peroxide, glutathione, pyridine nucleotides and ATP content were observed since symptom appearance (ES, 7 dpi). …”
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Genetic and physical mapping of the earliness perse locus Eps - Am1 in Triticum monococcum identifies early flowering 3 (ELF3) as a candidate gene por Tranquilli, Gabriela, Lewis, Silvina, Kippes, Nestor, Dubcovsky, Jorge, Alvarez, María Alejandra

“…Tetraploid wheat lines with combined truncation mutations in the A- and B-genome copies of ELF3 flowered earlier and had less spikelets per spike than the wild type control under SD and LD conditions. …”
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Genome-enabled insights into the biology of thrips as crop pests por Rotenberg, Dorith, Baumann, Aaron A., Ben-Mahmoud, Sulley, Christiaens, Olivier, Dermauw, Wannes, Ioannidis, Panagiotis, Jacobs, Chris G. C., Vargas Jentzsch, Iris M., Oliver, Jonathan E., Poelchau, Monica F., Priya Rajarapu, Swapna, Schneweis, Derek J., Snoeck, Simon, Taning, Clauvis N. T., Wei, Dong, Widana Gamage, Shirani M. K., Hughes, Daniel S. T., Murali, Shwetha C., Bailey, Samuel T., Bejerman, Nicolas Esteban, Holmes, Christopher J., Jennings, Emily C., Rosendale, Andrew J., Rosselot, Andrew, Hervey, Kaylee, Schneweis, Brandi A., Cheng, Sammy, Childers, Christopher, Simão, Felipe A., Dietzgen, Ralf Georg, Chao, Hsu, Dinh, Huyen, Vardhan Doddapaneni, Harsha, Dugan, Shannon, Han, Yi, Lee, Sandra L., Muzny, Donna M., Qu, Jiaxin, Worley, Kim C., Benoit, Joshua B., Friedrich, Markus, Jones, Jeffery W., Panfilio, Kristen A., Park, Yoonseong, Robertson, Hugh M., Smagghe, Guy, Ullman, Diane E., Van der Zee, Maurijn, Van Leeuwen, Thomas, Veenstra, Jan A., Waterhouse, Robert M., Weirauch, Matthew T., Werren, John H., Whitfield, Anna E., Zdobnov, Evgeny M., Gibbs, Richard A., Richards, Stephen

“…Benchmarking Universal Single-Copy Ortholog (BUSCO) assessments of the genome assembly (size = 415.8 Mb, scaffold N50 = 948.9 Kb) revealed a relatively complete and well-annotated assembly in comparison to other insect genomes. …”
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Rapid detection of tomato spotted wilt virus by real-time RT-LAMP and in-field application por Caruso, A. G., Ragona, A., Agrò, G., Bertacca, S., Yahyaoui, E., Galipienso, Luis, Rubio, Luis, Panno, Stefano, Davino, Salvatore

“…The developed real-time RT-LAMP assay proved to be one thousand and one hundred times more sensitive than end-point RT-PCR and real-time RT-PCR methods, respectively, detecting a total of 9.191 × 101 genome copies as minimum target, and no cross-reactivity were detected with other viruses belonging to Tospoviridae and Bromoviridae families used as outgroup. …”
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