Resultados de búsqueda - "Copi"

RDNA Barcodesystem por Götesson, Fredrik

“…The sequenced sample was able to copy the 28S large ribosomal subunit. The phylogenetic analysis with sequences from all classes showed that the sample belonged to the group of Pezizomycotina fungus. …”

Development of a ptp2-LAMP assay for the specific and sensitive detection of Nosema apis and its comparison with ptp3-LAMP for the detection of Nosema ceranae, in a region endemi... por Lannutti, Lucas, Gisder, Sebastian, Florin-Christensen, Monica, Genersch, Elke, Schnittger, Leonhard

“…The present study aimed to develop a LAMP protocol for N. apis based on the species-specific single copy polar tube protein 2 (ptp2) gene, and to analyze and compare its diagnostic performance with the previously developed polar tube protein 3 (ptp3) gene-based LAMP protocol for N. ceranae. …”
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A structural variant at ASIP associated with the darkness of hair coat is found in multiple zebu cattle populations por Trigo, B.B., Alves, N.F., Milanesi, M., Garcia, J.F., Terefe, Endashaw, Hanotte, Olivier H., Tijjani, Abdulfatai, Utsunomiya, Y.T.

“…Of 216 sequences analyzed, 63 zebu (45.9%) and five taurine (6.3%) animals had at least one copy of ASIP‐SV1. Four of the taurine animals presenting the SV were Romagnola cattle, a breed with history of zebu introgression. …”
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Susceptibility of TNF-α-deficient mice to Trypanosoma congolense is not due to a defective antibody response por Naessens, Jan, Kitani, H., Momotani, E., Sekikawa, K., Nthale, J.M., Iraqi, F.A.

“…C57BL/6 mice deficient in one or two copies of the gene for tumor necrosis factor alpha (TNF-α) were more susceptible to Trypanosoma congolense infection than their resistant, wild-type counterparts. …”
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Molecular cloning, expression and mapping of the translational initiation factor elF1 gene in Oryza sativa por Rangan, Latha, Rout, Anusuya, Sudarshan, Medhavi, Gregorio, Glenn

“…Southern analysis indicates that the gene is present in more than a single copy per haploid genome and mapped to chromosome 1 of rice. …”
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Real-time RT-PCR for quantitative detection of Plum pox virus por Olmos, Antonio, Bertolini, Edson, Gil, Maite, Cambra, Mariano

“…Although the two methods were able to detect as low as 4 copies of transcripts, only the TaqMan method maintained this sensitivity when plant material was used. …”
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Detection, discrimination and absolute quantitation of Tomato spotted wilt virus isolates using real time RT-PCR with TaqMan (R) MGB probes por Debreczeni, Diana E., Ruiz-Ruiz, Susana, Aramburu, Jose, Lopez, Carmelo, Belliure, Belén, Galipienso, Luis, Soler, Salvador, Rubio, Luis

“…Standard curves using RNA transcripts homologous to the three probes allowed reproducible quantitative assays with a wide dynamic range (10(3)-10(10) TSWV M segment RNA copies/ng of total RNA) and high sensitivity. This protocol was assayed with a battery of TSWV isolates, covering the range of the present known genetic variation, in single and/or mix infections in three plant hosts, as well as in the thrips vector Frankliniella occidenialis. …”
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Detection and absolute quantitation of watermelon mosaic virus by real-time RT-PCR with a TaqMan probe por Rubio, Luis, Giménez, Karen, Romero, Juan, Font-San-Ambrosio, Isabel, Alfaro-Fernández, Ana, Galipienso, Luis

“…A standard curve performed with transcripts enabled estimation of WMV RNA copies per ng of total RNA, with a wide dynamic range and sensitivity (104 to 1011). …”
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A look at trails through the pangenome visualization jungle por Durant, Éloi, Sabot, François, Conte, Matthieu, Rouard, Mathieu

“…Comparisons of such sequences showed that there are structural variations between individuals from the same species such as Copy Number Variations (CNV) and Presence Absence Variations (PAV) that can have a significant impact on phenotypic variation in plants and could be suitable for breeding improved crop varieties. …”
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Benchmark Study. The Agricultural Technology Development Fund for Contract Research: An INIA (Uruguay) Initiative por Hobbs, S. Huntington, Valverde, Carlos, Indarte, Eduardo, Lanfranco, Bruno

“…In presenting INIA's FPTA as a benchmark, ISNAR hopes to inspire research organizations in other developing countries to copy or adapt aspects of this experience with a view to improving their own agricultural research management practices and results.…”
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Using the transcriptome to annotate the genome revisited: application of massively parallel signature sequencing (MPSS) por Shah, Tushaar, Villiers, Etienne P. de, Nene, Vishvanath M., Hass, B., Taracha, E.A., Gardner, J.M., Sansom, C., Pelle, Roger, Bishop, Richard P.

“…More than 50% of the 60 putative single copy genes in T. parva that were absent from the genome of the closely related T. annulata had MPSS signatures. …”
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Characterisation of single domain ATP-binding cassette protein homologues of Theileria parva por Kibe, M.K., Macklin, M.D., Gobright, E.I., Bishop, Richard P., Urakawa, T., Ole-MoiYoi, O.K.

“…The second gene, TpABC2, also contains a single C-terminal ATP-binding motif. Copies of TpABC2 were present at four loci in the T. parva genome on three different chromosomes. …”
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Water losses in the inner delta of the River Niger: water balance and flooded area por Mahe, G., Bamba, F., Soumaguel, A., Orange, Didier, Olivry, J.C.

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A molecular genetic map of cassava (Manihot esculenta Crantz) por Fregene, Martin A., Angel, F., Gómez, R., Rodríguez, F., Chavarriaga Aguirre, Paul, Roca, W., Tohme, Joseph M., Bonierbale, Merideth W.

“…Six pairs of duplicated loci were detected by low-copy genomic and cDNA sequences used as probes. Efforts are underway to saturate the cassava map with additional markers, to join the male- and female-derived maps, and to elucidate genome organization in cassava.…”
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Ploidy manipulations and genetic markers as tools for analysis of quantitative trait variation in progeny derived from triploid plantains por Ortíz, R., Craenen, K., Vuylsteke, D.R.

“…This report shows the associated effects of ploidy and of the major loci controlling vegetative fruit parthenocarpy and resistance to black sigatoka fungal leaf spot on growth and yield characteristics of plantain‐banana euploid hybrids. The number of copies of the black sigatoka resistance allele (bs1) and of the fruit parthenocarpy gene (P1) in addition to their intralocus interaction and ploidy level were all found to significantly affect bunch and fruit characteristics in euploid plantain‐banana hybrids. …”
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Isothermal amplification for detection of Plum pox virus por Olmos, Antonio, Bertolini, Edson, Cambra, Mariano

“…The detection limit of the NASBA-FH was established at two copies of control transcripts, resulting 10 times higher than that obtained by Co-PCR and 1000 times higher than the sensitivity afforded by RT-PCR. …”
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Detection and quantitation of Citrus leaf blotch virus by TaqMan real-time RT-PCR por Ruiz-Ruiz, Susana, Ambrós, Silvia, Vives, María C., Navarro, Luis, Moreno, Pedro, Guerri, José

“…This protocol enabled detection of as little as 100 copies of CLBV RNA in various tissues and citrus varieties infected with CLBV sources from different geographical origins. …”
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Genetic resources of cacao: a catalogue of the CATIE collection por Engels, J.M.M.

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Seasonal variation in fungal biomass : the effects of soil temperature and moisture in a boreal forest por Leidefors, Malin

“…I amplified the ITS region using qPCR and quantified ITS copy number, which were then recalculated to get an estimation of fungal biomass in the sampled soil. …”

Sensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids por Zhou, Y., Zhang, J., Sun, H., Tao, D., Xu, B., Han, X., Ren, R., Ruan, J., Steinaa, Lucilla, Hemmink, Johanneke D., Han Jianlin, Li, X., Xu, J., Zhao, S., Xie, S., Zhao, C.

“…We found that this platform enabled a naked eye visual detection of ASFV at a detection limit as low as 2 copies/μL in 30 min. As expected, no cross-reactivity was observed with other porcine viruses. …”
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