Search Results - "CRISPR"

Introducing the non-rancidity trait into pearl millet seed : scenarios for achieving future impact in Kenya by Muindi, Pauline, Khaemba, Colleta, Rutsaert, Pieter, Donovan, Jason A., Bandyopadhyay, Anindya, Hearne, Sarah Jane

“…The International Maize and Wheat Improvement Center (CIMMYT) and partners are working to apply CRISPR technology to achieve a step-change in pearl millet seed product design: altering fatty acid metabolism to achieve the non-rancidity trait to create grain that when milled into flour has extended shelf life. …”
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Genetic Engineering: A Powerful Tool for Crop Improvement by Bhattacharjee, Mamta, Meshram, Swapnil, Dayma, Jyotsna, Pandey, Neha, Naglaa A, Abdallah, Hamwieh, Aladdin, Mahmoud, Nourhan Fouad, Acharjee, Sumita

“…Further, with the advent of technologies like zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas, it has been possible to overcome the limitations of conventional breeding techniques. …”
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The wheat sucrose synthase gene TaSus1 is a determinant of grain number per spike by Liping Shen, Lili Zhang, Changbin Yin, Xiaowan Xu, Yangyang Liu, Kuocheng Shen, He Wu, Zhiwen Sun, Ke Wang, Zhonghu He, Xueyong Zhang, Chenyang Hao, Jian Hou, Aoyue Bi, Xuebo Zhao, Daxing Xu, Botao Ye, Xuchang Yu, Ziying Wang, Danni Liu, Yuanfeng Hao, Fei Lu, Zifeng Guo

“…In the present study, we generated two independent mutants for the three TaSus1 homoeologs by CRISPR/Cas9-mediated genome editing. The triple mutants displayed lower FSN, GNS, grain number per spikelet (GNST), and TGW than wild-type plants. …”
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Multitrait engineering of Hassawi red rice for sustainable cultivation by Sedeek, Khalid, Mohammed, Nahed, Zhou, Yong, Zuccolo, Andrea, Sanikommu, Krishnaveni, Kantharajappa, Sunitha, Al-Bader, Noor, Tashkandi, Manal, Wing, Rod A., Mahfouz, Magdy M.

“…In addition, we generated the first high-quality reference genome and targeted the key flowering repressor gene, Hd4, thus shortening the plant's lifecycle and height. Using CRISPR/Cas9 multiplexing, we simultaneously disrupted negative regulators of flowering time (Hd2, Hd4, and Hd5), grain size (GS3), grain number (GN1a), and plant height (Sd1). …”
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Genome editing of an African elite rice variety confers resistance against endemic and emerging Xanthomonas oryzae pv. oryzae strains by Schepler-Luu, Van, Sciallano, Coline, Stiebner, Melissa, Ji, Chonghui, Boulard, Gabriel, Diallo, Amadou, Auguy, Florence, Char, Si Nian, Arra, Yugander, Schenstnyi, Kyrylo, Buchholzer, Marcel, Loo, Eliza PI, Bilaro, Atugonza L, Lihepanyama, David, Mkuya, Mohammed, Murori, Rosemary, Oliva, Ricardo, Cunnac, Sebastien, Yang, Bing, Szurek, Boris, Frommer, Wolf B

“…To protect African rice production against this emerging threat, we developed a hybrid CRISPR-Cas9/Cpf1 system to edit all known TALe-binding elements in three SWEET promoters of the East African elite variety Komboka. …”
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Broad-spectrum resistance to bacterial blight in rice using genome editing by Oliva, Ricardo, Ji, Chonghui, Atienza-Grande, Genelou, Huguet-Tapia, José C., Perez-Quintero, Alvaro, Li, Ting, Eom, Joon-Seob, Li, Chenhao, Nguyen, Hanna, Liu, Bo, Auguy, Florence, Sciallano, Coline, Luu, Van T., Dossa, Gerbert S., Cunnac, Sébastien, Schmidt, Sarah M., Slamet-Loedin, Inez H., Vera Cruz, Casiana, Szurek, Boris, Frommer, Wolf B., White, Frank F., Yang, Bing

“…The pathogen,Xanthomonas oryzaepv.oryzae(Xoo), secretes one or more of six known transcription-activator-like effectors (TALes) that bind specific promoter sequences and induce, at minimum, one of the three host sucrose transporter genesSWEET11,SWEET13andSWEET14, the expression of which is required for disease susceptibility. We used CRISPR–Cas9-mediated genome editing to introduce mutations in all threeSWEETgene promoters. …”
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Diagnostic kit for rice blight resistance by Eom, Joon-Seob, Luo, Dangping, Atienza-Grande, Genelou, Yang, Jungil, Ji, Chonghui, Thi Luu, Van, Huguet-Tapia, José C., Char, Si Nian, Liu, Bo, Nguyen, Hanna, Schmidt, Sarah Maria, Szurek, Boris, Vera Cruz, Casiana, White, Frank F., Oliva, Ricardo, Yang, Bing, Frommer, Wolf B.

“…Specifically, we include aSWEETpromoter database, RT–PCR primers for detectingSWEETinduction, engineered reporter rice lines to visualize SWEET protein accumulation and knock-out rice lines to identify virulence mechanisms in bacterial isolates. We also developed CRISPR–Cas9 genome-edited Kitaake rice to evaluate the efficacy of EBE mutations in resistance, software to predict the optimal resistance gene set for a specific geographic region, and two resistant ‘mega’ rice lines that will empower farmers to plant lines that are most likely to resist rice blight.…”
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A model to incorporate the bHLH transcription factor OsIRO3 within the rice iron homeostasis regulatory network by Carey-Fung, Oscar, O'Brien, Martin, Beasley, Jesse T., Johnson, Alexander A. T.

“…Recent investigation of the rice Fe homeostasis network revealed OsIRO3, a basic Helix–Loop–Helix (bHLH) TF as a putative negative regulator of genes involved in Fe uptake, transport, and storage. We employed CRISPR-Cas9 gene editing to target the OsIRO3 coding sequence and generate two independent T-DNA-free, loss-of-function iro3 mutants in rice cv. …”
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Bridging Genes and Sensory Characteristics in Legumes: Multi-Omics for Sensory Trait Improvement by Sharma, Niharika, Mukhopadhyay, Soumi Paul, Onkarappa, Dhanyakumar, Yogendra, Kalenahalli, Ratanpaul, Vishal

“…Furthermore, a molecular understanding of sensory pathways opens avenues for precise gene editing (e.g., using CRISPR-Cas9) to modify specific genes, reduce off-flavour compounds or optimise texture. …”
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Why choose the rabbit to work in reproductive technology? by Marco-Jiménez, Francisco, Viudes-De-Castro, María P., Vicente, José S.

“…The incorporation of cutting-edge genomic editing tools, such as CRISPR/Cas9, has reestablished rabbits as essential models in genetic and biomedical research, driving scientific progress. …”
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The role of terpenes produced by Brassica napus in interactions with Verticillium longisporum by Ruffino, Alessandra

“…Finally, to produce rapeseed resistant plants to V. longisporum a clustered regulatory interspaced short palindromic repeats (CRISPR/Cas9) construct to was generated to produce rapeseed plants lacking the homologous AT5G44630 gene.…”
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Selecting for useful properties of plants and fungi – Novel approaches, opportunities, and challenges by Kersey, Paul J., Collemare, Jerome, Cockel, Christopher P., Das, Dibakar, Dulloo, Mohammad Ehsan, Kelly, Laura J., Lettice, Eoin, Malécot, Valery, Maxted, Nigel, Metheringham, Carey, Thormann, Imke, Leitch, Ilia J.

“…Improvements in DNA sequencing technologies have enhanced our capacity to identify and manipulate genetic diversity, increasing the range of variation that can be utilized, and accelerating the breeding cycle to reduce the time taken to develop and put new varieties to use. Most recently, the CRISPR/Cas9 gene editing technology has greatly increased our capacity to directly introduce novel genetic variants without unwanted associated material. …”
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A novel Pik allele confers extended resistance to rice blast by Qi, Zhongqiang, Meng, Xiuli, Xu, Ming, Du, Yan, Yu, Junjie, Song, Tianqiao, Pan, Xiayan, Zhang, Rongsheng, Cao, Huijuan, Yu, Mina, Telebanco-Yanoria, Mary Jeanie, Lu, Guodong, Zhou, Bo, Liu, Yongfeng

“…Pik‐W25 conferred resistance to isolates expressing AvrPik‐C/D/E alleles. CRISPR‐Cas9 editing was used to generate transgenic lines with a loss of function in Pik‐W25‐1 and Pik‐W25‐2, resulting in loss of resistance in G9 to isolates expressing the three alleles, confirming that Pik‐W25‐induced immunity required both Pik‐W25‐1 and Pik‐W25‐2. …”
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Self-(In)compatibility Systems: Target Traits for Crop-Production, Plant Breeding, and Biotechnology by Munoz-Sanz, Juan V., Zuriaga, Elena, Cruz-García, Felipe, McClure, Bruce, Romero, Carlos

“…Molecular level studies have enabled SI to SC transitions (and vice versa) to be intentionally manipulated using marker assisted breeding and targeted approaches based on transgene integration, silencing, and more recently CRISPR knock-out of SI-related factors. These scientific advances have, in turn, provided a solid basis to implement new crop production and plant breeding practices. …”
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¿Cómo funciona?

“…El proceso de edición génica mediante CRISPR-Cas necesita tres componentes principales: 1) una secuencia de adn o gen que se quiere modificar; 2) una secuencia de arn que sirve de guía para reconocer el gen, y 3) la proteína Cas o “tijera molecular” para realizar los cortes del adn. …”
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¿Qué aplicaciones hay en la alimentación y agricultura?

“…En la figura 13, se presentan seis ejemplos de variedades convencionales obtenidas a base de crispr en Norte América y dos ejemplos en Argentina y Colombia.…”
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From the basics to emerging diagnostic technologies: What is on the horizon for tilapia disease diagnostics? by Ha Thanh Dong, Chaijarasphong, T., Barnes, A.C., Delamare-Deboutteville, Jerome, Lee, P.A., Senapin, S., Mohan, Chadag V., Tang, K.F.J., McGladdery, S.E., Bondad-Reantaso, M.G.

“…The review also covers current and emerging molecular diagnostic technologies for tilapia pathogens including polymerase chain reaction methods (conventional, quantitative, digital), isothermal amplification methods Loop-mediated Isothermal Amplification (LAMP), recombinase polymerase amplification (RPA), clustered regularly interspaced short palindromic repeats (CRISPR)-based detection, lateral flow immunoassays, as well as discussing what is on the horizon for tilapia disease diagnostics (next generation sequencing, artificial intelligence, environmental Deoxyribonucleic Acid (DNA) and Ribonucleic Acid (RNA) and point-of-care testing) providing a future vision for transferring these technologies to farmers and stakeholders for a sustainable aquatic food system transformation.…”
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Transboundary animal diseases: Research and development priorities for resilient agrifood systems by Hung Nguyen-Viet

“…Recent research activities include using information and communication technology to improve early detection and response for control of African swine fever, foot-and-mouth disease and Newcastle disease in Vietnam; assessing the epidemiology and control of peste des petits ruminants in East and West Africa, and using CRISPR–Cas9 technology to develop vaccines against African swine fever. …”
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Food system innovations and digital technologies to foster productivity growth and rural transformation by Benfica, Rui, Chambers, Judith A., Koo, Jawoo, Nin-Pratt, Alejandro, Falck-Zepeda, José B., Stads, Gert-Jan, Arndt, Channing

“…Given their transformative potential and the urgency of developing the enabling R&D and trajectories required for impact, we highlight genome editing bio-innovations, specifically CRISPR-Cas9, to address sustainable agricultural growth; and digital technologies, including remote sensing, connected sensors, artificial intelligence, digital advisory services, digital financial services, and e-commerce, to help guide the operations and decision-making of farmers, traders, and policymakers in agricultural value chains. …”
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ARGONAUTE 2 increases rice susceptibility to rice black-streaked dwarf virus infection by epigenetically regulating HEXOKINASE 1 expression by Wang, Zhaoyun, Chen, Dongyue, Sun, Feng, Guo, Wei, Wang, Wei, Li, Xuejuan, Lan, Ying, Du, Linlin, Li, Shuo, Fan, Yongjian, Zhou, Yijun, Zhao, Hongwei, Zhou, Tong

“…Using transgenic rice plants overexpressing OsAGO2 and Osago2 mutants generated through transposon‐insertion or CRISPR/Cas9 technology, we found that overexpression of OsAGO2 enhanced rice susceptibility to RBSDV infection. …”
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