Resultados de búsqueda - "CRISPR"

Novel Genome-Editing Approaches for Developing Non-GM Crops for Sustainable Improvement and the Mitigation of Climate Changes por Naglaa A, Abdallah, Hamwieh, Aladdin, Baum, Michael

“…Also, viral vectors were used for the delivery of CRISPR/Cas components to obtain transgene-free edited plants. …”
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A new and novel high-fidelity genome editing tool for banana using Cas-CLOVER por Tripathi, L., Ntui, V.O., Tripathi, J., Norman, D., Crawford, J.

“…To date, three main types of engineered nucleases have been customized for genome editing: zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN) and clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas). Among all the genome editing technologies, CRISPR/Cas9, which is based on bacterial immunity against viral invasion and phage, has been adopted as the most popular technique for functional genomics and crop improvement due to its ease of use, design flexibility, high efficiency and multiplexing (Tripathi et al., 2020). …”
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Advancements and challenges in gene editing for improvement of vegetatively propagated crops por Tripathi, J.N., Muiruri, S., Tripathi, L.

“…Gene editing technologies, particularly CRISPR-Cas9, have revolutionized agriculture by offering precise and efficient tools to enhance crop production. …”
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Edición de genes en plantas: definiciones y aplicaciones en el cultivo de la papa en AGROSAVIA y el INTA por Garzón Martínez, Gina Alessandra, Jiménez Ortega, Pablo Edgar, Massa, Gabriela, Feingold, Sergio, Barrero, Luz Stella

“…La presente cartilla explica de manera sencilla la edición de genes en plantas para la alimentación y la agricultura, con énfasis en la tecnología CRISPR-Cas, el método más utilizado actualmente. Se describen los conceptos generales, los componentes necesarios y el proceso, desde la regeneración de plantas hasta la introducción de los elementos de edición en las plantas. …”
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Genome editing in banana: tools, techniques, and challenges por Tripathi, J., Kariuki, S.M., Waweru, D.N., Tripathi, L.

“…Among these, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based tools stand out for their significant advantages, though they also present regulatory and technical challenges. …”
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Improved eating and cooking quality of indica rice cultivar YK17 via adenine base editing of Wx(a) allele of Granule-Bound Starch Synthase I (GBSS I) por Monsur, Mahmuda Binte, Ni, Cao, Xiangjin, Wei, Lihong, Xie, Guiai, Jiao, Tang, Shaoqing, Sreenivasulu, Nese, Gaoneng, Shao, Peisong, Hu

“…Recent work has improved ECQ of japonica rice via clustered regularly interspaced short palindromic repeats/CRISPR- associated protein 9 (CRISPR/Cas9) or cytosine base editing (CBE) techniques. …”
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Conclusiones y perspectivas

“…La tecnología CRISPR-Cas se utiliza en diversos países para mejorar los cultivos de plantas y para muchas otras aplicaciones en agricultura, medicina e industria. …”
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¿Qué es la edición de genes?

“…Hay varios métodos de edición de genes, pero aquí nos referiremos al más utilizado, que es conocido como CRISPR-Cas. El científico español Francis Mojica, en 1993, descubrió el mecanismo por el cual las bacterias se defienden de los virus y lo llamó el sistema crispr. …”
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Agrobacterium tumefaciens–mediated genome editing in banana por Tripathi, J., Tripathi, L.

“…Key steps include callus induction, ECS generation and maintenance, transformation using plasmid construct containing genome editing reagents such as CRISPR/Cas9, and regeneration. The method ensures the editing of target genes in the banana genome, facilitating genetic improvements.…”
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Draft genome sequence of Escherichia coli MTR_GS_S1457 strain isolated from a soil sample of a vegetable garden in Gazipur, Bangladesh por Islam, Md. Saiful, Pramanik, Pritom Kumar, Rana, Md. Liton, Ullah, Md. Ashek, Neloy, Fahim Haque, Ramasamy, Srinivasan, Schreinemachers, Pepijn, Oliva, Ricardo, Rahman, Md. Tanvir

“…With a length of 4,918,647 bp, this strain contained one plasmid, two CRISPR arrays, 54 predicted antibiotic resistance genes, and 81 predicted virulence factor genes.…”
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Precision genetics tools for genetic improvement of banana por Tripathi, J., Ntui, V.O., Tripathi, L.

“…Genome editing, particularly CRISPR/Cas, offers precise modifications to the banana genome and has been successfully applied in the improvement of banana. …”
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Tissue-specific enhancement of OsRNS1 with root-preferred expression is required for the increase of crop yield por Gho, Yun-Shil, Choi, Heebak, Moon, Sunok, Kim, Sung-Ryul, Ha, Sun-Hwa, Jung, Ki-Hong

“…This study aimed to verify the usefulness of a new rice root-preferred promoter to optimize the function of a target gene with root-preferred expression in rice. osrns1 mutant had defects in root development based on T-DNA insertional mutant screening and CRISPR technology. To optimize the function of OsRNS1, we generated OsRNS1-overexpression plants under two different promoters: a whole-plant expression promoter and a novel root-preferred expression promoter. …”
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Introducción y desarrollo de variedades de algodón Upland en el sistema productivo colombiano: una revisión por Burbano Figueroa, Oscar, Montes Mercado, Karen Sofía, Pastrana Vargas, Iván Javier, Cadena Torres, Jorge

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Multi-omics resources for targeted agronomic improvement of pigmented rice por Sedeek, Khalid, Zuccolo, Andrea, Fornasiero, Alice, Weber, Annika M., Sanikommu, Krishnaveni, Sampathkumar, Sangeetha, Rivera, Luis F., Butt, Haroon, Mussurova, Saule, Alhabsi, Abdulrahman, Nurmansyah, Nurmansyah, Ryan, Elizabeth P., Wing, Rod A., Mahfouz, Magdy M.

“…We established a regeneration and transformation system and used CRISPR–Cas9 to knock out three flowering time repressors (Hd2, Hd4 and Hd5) in the black Indonesian rice Cempo Ireng, resulting in an early maturing variety with shorter stature. …”
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Somatic embryogenesis in coffee: the evolution of biotechnology and the integration of omics technologies offer great opportunities por Campos, N.A., Panis, Bartholomeus, Carpentier, Sebastien C.

“…While genetic transformation got many attention in the past and is booming with the CRISPR technology, micropropagation and SE are still the major bottle neck and urgently need more attention. …”
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Application of genetic modification and genome editing for developing climate-smart banana por Tripathi, L., Ntui, V.O., Tripathi, J.N.

“…Genome editing, an emerging powerful tool, can be applied for developing sustainable solutions to adapt to climate change by resisting biotic and abiotic stresses. CRISPR/Cas9‐based genome editing has been lately established for banana, paving the way for functional genomics allowing identification of genes associated with stress‐tolerant traits, which could be used for the improvement of banana for adaptation to a changing climate. …”
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In situ deletions reveal regulatory components for expression of an intracellular immune receptor gene and its co‐expressed genes in Arabidopsis por Yu, Huiyun, Yang, Leiyun, Li, Zhan, Sun, Feng, Li, Bo, Guo, Shengsong, Wang, Yong-Fei, Zhou, Tong, Hua, Jian

“…Despite intensive studies of the regulation of NLR proteins, cis‐acting elements for NLR gene induction, repression or co‐expression are largely unknown due to a larger than usual cis‐region for their expression regulation. Here we used the CRISPR/Cas9 genome editing technology to generate a series of in situ deletions at the endogenous location of an NLR gene SNC1 residing in the RPP5 gene cluster. …”
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Breeding for High Oleate Oilseed Crops: Opportunities, Constraints, and Prospects por Gangurde, Sunil S., Bhat, Ramesh S., Shirasawa, Kenta, Varshney, Rajeev K., Pandey, Manish K.

“…Genetic engineering and gene editing technologies, such as CRISPR/Cas9, offer transformative prospects for modifying fatty acid biosynthesis pathways. …”
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Consumers perception of genetically modified horticultural food : an interview study por Persson, Fredrik

“…Also, the perception of related technology, the gene-editing technology CRISPR/Cas was investigated. This gene-editing technology is one of the most promising technologies and with precision alter DNA in crops today, and therefore, perception towards this technology is highly relevant to investigate. …”

Targeted knockout of early nodulin-like 3 (MusaENODL3) gene in banana reveals its function in resistance to Xanthomonas wilt disease por Ntui, V.O., Tripathi, J., Shah, T., Tripathi, L.

“…To further unravel the role of the ENODL gene in disease resistance, the CRISPR/Cas9 system was employed to disrupt the MusaENODL3 gene in 'Gonja Manjaya' precisely. …”
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