Search Results - Subculture.

Phase-variable expression of the biofilm-associated protein (Bap) in Staphylococcus aureus. by Tormo-Mas, María A., Ubeda, Carles, Marti, Miguel, Maiques, Elisa, Cucarella, Carme, Valle, Jaione, Foster, Timothy J., Lasa, Inigo, Penadés, José R.

“…The biofilm-negative phenotype reverted to wild-type at a similar frequency upon subculture of Bap-negative variants in liquid media. …”
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Assessing genetic relatedness of in vitro cassava accessions using morphological traits and DArTseq single nucleotide polymorphism (SNP) markers by Byiringiro, M.H., Uchendu, E.E., Paliwal, R., Abberton, M.

“…Morphological traits (plant height, root length, number of roots per plant, number of leaves per plant, leaf length, leaf width, and lobe number) were quantified weekly for four weeks post-meristem subculture, commencing one week after subculture. Genome-wide SNP markers were generated using the DArTseq Genotype-by-Sequencing approach, yielding 19,467 high-quality SNPs. …”
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Trypanosoma brucei brucei: In vitro production of metacyclic forms by Hirumi, H., Hirumi, K., Moloo, S.K., Shaw, M.K.

“…When the duration of the subculture was prolonged to 17 days or more at 27° C, the metacyclic forms decreased in number while short trypomastigotes, long slender epimastigotes, and long slender trypomastigotes increased in number. …”
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Growth and Morphogenesis of Citrus Tissue-Cultures Infected with Psorosis, Vein Enation, and Cachexia by Durán-Vila, Núria, Medina, Vicente, Pina, José A., Ortega, Carmen, Molins, M., Navarro, Luis

“…The amount and morphology of secondary callus after the first subculture were similar in infected and uninfected tissues. …”
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Cryopreservation of Digitalis obscura selected genotypes by encapsulation-dehydration by Sales, Ester, Nebauer, Sergio G., Arrillaga, I., Segura, J.

“…RAPID markers were employed to evaluate possible somaclonal variation occurring in shoots regenerated through successive subcultures and after cryo preservation. The band patterns revealed differences between the original parent plant and the shoots grown in vitro, especially after a prolonged subculture (84.9% of matches for HU3 after 16 subcultures vs. 93.4% for AY3 after 2 subcultures). …”
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”Vi är ju en egen värld” by Gauffin, Kerstin

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The earthship concept by Ekvall, Martin

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Efficacy of chemotherapy and thermotherapy in elimination of east African cassava mosaic virus from Tanzanian cassava landrace by Kidulile, C.E., Ateka, E.M., Alakonya, A.E., Ndunguru, J.C.

“…Plantlets of plants positive for EACMV were initiated in Murashige and Skoog (MS) medium. On the second subculture, they were subjected into chemical treatment in the medium containing salicylic acid (0, 10, 20, 30 and 40 mg/L) and ribavirin (0, 5, 10, 15 and 20 mg/L). …”
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Effect of Gas Exchange Rate, Vessel Type, Planting Density, and Genotype on Growth, Photosynthetic Activity, and Ion Uptake of In Vitro Potato Plants by Vollmer, R., Espirilla, J., Espinoza, A., Villagaray, R., Castro, M., Pineda, S., Sánchez, J.C., Mello, A.F.S., Azevedo, V.C.R.

“…Depending on the purpose of the subculture of in vitro plants, their growth and development can be molded using different gas exchange rates, planting densities, and vessel volumes.…”
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Performances of plantlets from selected cassava (Manihot esculenta Crantz) genotypes under Semi-Autotrophic Hydroponics (SAH) using different substrates by Mamy, B.M., Kokou, K., Sikirou, M., Adetoro, N., Angelique, K., Nyende, A.B.

“…Data were collected on survival, height, leaf and internode numbers, and cutting numbers at the end of each subculture period. The data were analysed using ANOVA in R software. …”
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Efecto de subcultivos sucesivos de Beauveria bassiana sobre sus características y actividad contra Premnotrypes vorax by García, Magda X., Villamizar, Laura F., Torres, Lissette A., Cotes, Alba M.

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Performance of cassava planting materials produced using the "Semi Autotrophic Hydroponic" technology in the laboratory and across locations in the Democratic Republic of Congo by Binzunga, M.M.

“…Experiment 1, employing a split-plot design, used four genotypes (IB961089A, MM060083, Nase14, and Albert28) and four single substrates: KlasmannTS3 (K), Vermiculite (V), Local Peat (P), and Sawdust (S). It involved three subculture periods lasting four weeks, with data collection on survival, height, leaf, internode, and cutting numbers. …”
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Arbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplication by Covacevich, Fernanda, Hernandez Guijarro, Keren, Crespo, Esteban, Lumini, Erica, Rivero Mega, María Soledad, Lugo, Mónica Alejandra

“…Indigenous propagules from two pristine sites at 3870 and 3370 m of elevation were multiplied using different host plants; propagation periods (2–6 months), and subculture cycles (1; 2; or 3) from 5 to 13 months. …”
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Avances de investigación para la propagación vegetativa por cultivo in vitro de Myrciaria dubia (H.B.K.) Mc Vaugh “camu camu” by Pinedo Freyre, Sergio Fernando, Imán Correa, Sixto Alfredo, Celis Morey, Eloiza

“…The best medium for the multiplication phase is M&S + 2.5 mg/L BAP + 0.1 mg/L NAA, 80% survival at 30 days after subculture.…”
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Nyskapande och utvecklande parkskötsel genom reflekterande praktik by Gustavsson, Oscar

State of the art of genetic engineering in potato: from the first report to its future potential by Nahirñak, Vanesa, Almasia, Natalia Ines, Gonzalez, Matías Nicolás, Massa, Gabriela Alejandra, Decima Oneto, Cecilia Andrea, Feingold, Sergio Enrique, Hopp, Horacio Esteban, Vazquez Rovere, Cecilia

“…Several critical factors affect the transformation efficiency: vector type, insert size, Agrobacterium strain, explant type, composition of the subculture media, selective agent, among others. Moreover, transient or stable transformation, constitutive or inducible promoters, antibiotic/herbicide resistance or marker-free strategies can be considered. …”
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Virus Content and Growth Patterns of Callus Cultured in vitro from Healthy and Virus-Infected Citrus Species by Durán-Vila, Núria, Cambra, Mariano, Pina, José A., Ballester-Olmos, José F., Navarro, Luis

“…The growth of secondary callus during subculturing was similar in healthy and infected tissues. …”
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Embryogenesis in vitro of several Citrus species and cultivars by Perez, Rosa, Galiana, AM, Navarro, Luis, Durán-Vila, Núria

“…The small proliferation of callus and globular bodies recovered from the primary cultures were suitable for recovering embryogenie callus lines by periodical subculturing into fresh medium. After several monthly subcultures discarding embryos and “pseudobulbi”, cell lines virtually devoid of organized structures were obtained from three cultivars of sweet orange, sour orange and Cleopatra mandarin. …”
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In vitro cultivation of Trypanosoma congolense bloodstream forms in the absence of feeder cell layers by Hirumi, H., Hirumi, K.

“…Every 4–5 days, the attached bloodstream forms were resuspended in fresh medium by gentle pipetting and then were subcultured. The method was further simplified by initiating primary cultures directly with 10 μl of the tail blood of infected mice in 24-well culture plates and then by subcultivating either in wells or in T-25 flasks. …”
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Production and cryoconservation of embryogenic cultures of mandarin and mandarin hybrids by Perez, Rosa, Mas, O., Navarro, Luis, Durán-Vila, Núria

“…The small proliferations of nucellar callus from cultured ovules were suitable to recover embryogenic callus lines by periodical subculturing to fresh medium. The embryogenic callus cultures were subjected to cryoprotection with 10% (v/v) (DMSO), freezing by slow cooling, storage in liquid nitrogen and thawing by fast warming. …”
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