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  1. TREC-IN: gene knock-in genetic tool for genomes cloned in yeast by Chandran, S., Noskov, V.N., Segall-Shapiro, T.H., Ma, L., Whiteis, C., Lartigue, C., Jores, Joerg, Vashee, S., Ray-Yuan Chuang

    Published 2014
    “…To overcome this issue, we incorporated a split marker system into the TREC method, enabling seamless gene knock-in with high efficiency. The modified method is called TREC-assisted gene knock-in (TREC-IN). …”
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    Journal Article
  2. Generating large-scale conditional knock-in mouse models using CRISPR/Cas9 in embryonic stem by Mcfarlane, Gus Rowan

    Published 2016
    “…The application of CRISPR/Cas9 in mouse embryonic stem cells represents a highly efficient and wellestablished method for generating large-scale conditional knock-in mouse models. This study employed a CRISPR/Cas9-mediated gene targeting approach in mouse embryonic stem cells to introduce a conditional knock-in construct within intron 11 of the Fbxw7 gene. …”
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    Second cycle, A2E

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