In vivo neutralization of the dendrotoxin-mediated neurotoxicity of black mamba venom by oligoclonal human IgG monoclonal antibodies
The black mamba (Dendroaspis polylepis) is one of the most feared snake species of the African savanna. It has a potent, fast-acting neurotoxic venom comprised of dendrotoxins and α-neurotoxins associated with high fatality in untreated victims. Current antivenoms are both scarce on the African c...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2019
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| Subjects: | |
| Online Access: | https://www.nature.com/articles/s41467-018-06086-4 https://hdl.handle.net/10669/76386 |
| Summary: | The black mamba (Dendroaspis polylepis) is one of the most feared snake species of the
African savanna. It has a potent, fast-acting neurotoxic venom comprised of dendrotoxins and
α-neurotoxins associated with high fatality in untreated victims. Current antivenoms are both
scarce on the African continent and present a number of drawbacks as they are derived from
the plasma of hyper-immunized large mammals. Here, we describe the development of an
experimental recombinant antivenom by a combined toxicovenomics and phage display
approach. The recombinant antivenom is based on a cocktail of fully human immunoglobulin
G (IgG) monoclonal antibodies capable of neutralizing dendrotoxin-mediated neurotoxicity of
black mamba whole venom in a rodent model. Our results show the potential use of fully
human monoclonal IgGs against animal toxins and the first use of oligoclonal human IgG
mixtures against experimental snakebite envenoming. |
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