Genomic characterization of antimicrobialresistance and virulence factors in Salmonella isolates obtained from pig farms in Antioquia, Colombia

Occurrence of antimicrobial-resistant Salmonella strains has been reported worldwide, because of inappropriate use of antimicrobial products in either humans or animals. The presence of multidrug resistant Salmonella in pig production systems had been reported in Antioquia, Colombia. Aim: To identif...

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Autores principales:	García Álvarez, María Isabel, Vidal, Juana L., Donado Godoy, Pilar, Smith, Jared, Shariat, Nikki, Valencia, María Fernanda, Gómez Osorio, Luis M., López Osorio, Sara, Chaparro Gutiérrez, Jenny J.
Formato:	article
Lenguaje:	Inglés
Publicado:	Public Library of Science 2025
Materias:	Enfermedades de los animales - L73 Salmonella Antimicrobiano Cerdo cebón Explotación agraria Ganadería y especies menores http://aims.fao.org/aos/agrovoc/c_6757 http://aims.fao.org/aos/agrovoc/c_509 http://aims.fao.org/aos/agrovoc/c_59e0f842 http://aims.fao.org/aos/agrovoc/c_2809
Acceso en línea:	https://pubmed.ncbi.nlm.nih.gov/39888971/ http://hdl.handle.net/20.500.12324/41148

id	RepoAGROSAVIA41148
record_format	dspace
institution	Corporación Colombiana de Investigación Agropecuaria
collection	Repositorio AGROSAVIA
language	Inglés
topic	Enfermedades de los animales - L73 Salmonella Antimicrobiano Cerdo cebón Explotación agraria Ganadería y especies menores http://aims.fao.org/aos/agrovoc/c_6757 http://aims.fao.org/aos/agrovoc/c_509 http://aims.fao.org/aos/agrovoc/c_59e0f842 http://aims.fao.org/aos/agrovoc/c_2809
spellingShingle	Enfermedades de los animales - L73 Salmonella Antimicrobiano Cerdo cebón Explotación agraria Ganadería y especies menores http://aims.fao.org/aos/agrovoc/c_6757 http://aims.fao.org/aos/agrovoc/c_509 http://aims.fao.org/aos/agrovoc/c_59e0f842 http://aims.fao.org/aos/agrovoc/c_2809 García Álvarez, María Isabel Vidal, Juana L. Donado Godoy, Pilar Smith, Jared Shariat, Nikki Valencia, María Fernanda Gómez Osorio, Luis M. López Osorio, Sara Chaparro Gutiérrez, Jenny J. Genomic characterization of antimicrobialresistance and virulence factors in Salmonella isolates obtained from pig farms in Antioquia, Colombia
description	Occurrence of antimicrobial-resistant Salmonella strains has been reported worldwide, because of inappropriate use of antimicrobial products in either humans or animals. The presence of multidrug resistant Salmonella in pig production systems had been reported in Antioquia, Colombia. Aim: To identify antimicrobial resistance genes (ARG) in different Salmonella spp. strains isolated from pig productions in Antioquia, Colombia. Methods: Samples were received at the Diagnostic Unit of the Faculty of Agrarian Sciences at the University of Antioquia, from January 1, 2019, to January 2021. A total of 28 isolates of Salmonella spp. were included, which presented phenotypic resistance to more than one antibiotic used in pig farms. Whole genome sequencing (WGS) was performed in the Unit of Genomic of Agrosavia using an automated pipeline from the GHRU- Sanger Institute, employing the Illumina MiSeq platform. Results: WGS revealed 34 ARGs among these isolates. In 25 isolates (89%) more than two ARGs were found. Genes encoding resistance were found for 10 different groups of antibiotics (beta-lactam, aminoglycosides, chloramphenicol, rifampicins, lincosamides, fluoroquinolones, tetracyclines, sulfonamides and trimethoprim). The most frequently observed MDR profile in Typhimurium isolates was AMP-CEX-CEP-CEF-EFT-CEQ-FLU-ENR-TE-FFC-SXT.
format	article
author	García Álvarez, María Isabel Vidal, Juana L. Donado Godoy, Pilar Smith, Jared Shariat, Nikki Valencia, María Fernanda Gómez Osorio, Luis M. López Osorio, Sara Chaparro Gutiérrez, Jenny J.
author_facet	García Álvarez, María Isabel Vidal, Juana L. Donado Godoy, Pilar Smith, Jared Shariat, Nikki Valencia, María Fernanda Gómez Osorio, Luis M. López Osorio, Sara Chaparro Gutiérrez, Jenny J.
author_sort	García Álvarez, María Isabel
title	Genomic characterization of antimicrobialresistance and virulence factors in Salmonella isolates obtained from pig farms in Antioquia, Colombia
title_short	Genomic characterization of antimicrobialresistance and virulence factors in Salmonella isolates obtained from pig farms in Antioquia, Colombia
title_full	Genomic characterization of antimicrobialresistance and virulence factors in Salmonella isolates obtained from pig farms in Antioquia, Colombia
title_fullStr	Genomic characterization of antimicrobialresistance and virulence factors in Salmonella isolates obtained from pig farms in Antioquia, Colombia
title_full_unstemmed	Genomic characterization of antimicrobialresistance and virulence factors in Salmonella isolates obtained from pig farms in Antioquia, Colombia
title_sort	genomic characterization of antimicrobialresistance and virulence factors in salmonella isolates obtained from pig farms in antioquia, colombia
publisher	Public Library of Science
publishDate	2025
url	https://pubmed.ncbi.nlm.nih.gov/39888971/ http://hdl.handle.net/20.500.12324/41148
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spelling	RepoAGROSAVIA411482025-08-29T03:01:14Z Genomic characterization of antimicrobialresistance and virulence factors in Salmonella isolates obtained from pig farms in Antioquia, Colombia Genomic characterization of antimicrobialresistance and virulence factors in Salmonella isolates obtained from pig farms in Antioquia, Colombia García Álvarez, María Isabel Vidal, Juana L. Donado Godoy, Pilar Smith, Jared Shariat, Nikki Valencia, María Fernanda Gómez Osorio, Luis M. López Osorio, Sara Chaparro Gutiérrez, Jenny J. Enfermedades de los animales - L73 Salmonella Antimicrobiano Cerdo cebón Explotación agraria Ganadería y especies menores http://aims.fao.org/aos/agrovoc/c_6757 http://aims.fao.org/aos/agrovoc/c_509 http://aims.fao.org/aos/agrovoc/c_59e0f842 http://aims.fao.org/aos/agrovoc/c_2809 Occurrence of antimicrobial-resistant Salmonella strains has been reported worldwide, because of inappropriate use of antimicrobial products in either humans or animals. The presence of multidrug resistant Salmonella in pig production systems had been reported in Antioquia, Colombia. Aim: To identify antimicrobial resistance genes (ARG) in different Salmonella spp. strains isolated from pig productions in Antioquia, Colombia. Methods: Samples were received at the Diagnostic Unit of the Faculty of Agrarian Sciences at the University of Antioquia, from January 1, 2019, to January 2021. A total of 28 isolates of Salmonella spp. were included, which presented phenotypic resistance to more than one antibiotic used in pig farms. Whole genome sequencing (WGS) was performed in the Unit of Genomic of Agrosavia using an automated pipeline from the GHRU- Sanger Institute, employing the Illumina MiSeq platform. Results: WGS revealed 34 ARGs among these isolates. In 25 isolates (89%) more than two ARGs were found. Genes encoding resistance were found for 10 different groups of antibiotics (beta-lactam, aminoglycosides, chloramphenicol, rifampicins, lincosamides, fluoroquinolones, tetracyclines, sulfonamides and trimethoprim). The most frequently observed MDR profile in Typhimurium isolates was AMP-CEX-CEP-CEF-EFT-CEQ-FLU-ENR-TE-FFC-SXT. Porcicultura 2025-08-28T19:44:35Z 2025-08-28T19:44:35Z 2025-01 2025 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 https://pubmed.ncbi.nlm.nih.gov/39888971/ 1935-2727 http://hdl.handle.net/20.500.12324/41148 10.1371/journal.pntd.0012830 reponame:Biblioteca Digital Agropecuaria de Colombia instname:Corporación colombiana de investigación agropecuaria AGROSAVIA eng PLoS Neglected Tropical Diseases 19 1 1 18 Wibisono FM, Wibisono FJ, Effendi MH, Plumeriastuti H, Hidayatullah AR, Hartadi EB, et al. 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Genomic characterization of antimicrobialresistance and virulence factors in Salmonella isolates obtained from pig farms in Antioquia, Colombia

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