Electronic Resource

Peptide HER2(776-788) represents a naturally processed broad MHC class II-restricted T cell epitope.

Bibliographic Details
Title: Peptide HER2(776-788) represents a naturally processed broad MHC class II-restricted T cell epitope.
Source: British Journal of Cancer, 85 (10
Publisher Information: 2001-11
Added Details: Sotiriadou, R
Perez, S A
Gritzapis, A D
Sotiropoulou, Panagiota
Echner, H
Heinzel, S
Mamalaki, A
Pawelec, Graham
Voelter, W
Baxevanis, C N
Papamichail, Michael
Document Type: Electronic Resource
Abstract: HER2/neu-derived peptides inducing MHC class II-restricted CD4+ T helper lymphocyte (Th) responses, although critical for tumour rejection, are not thoroughly characterized. Here, we report the generation and characterization of CD4+ T cell clones specifically recognizing a HER-2/neu-derived peptide (776-788) [designated HER2(776-788)]. Such clones yielded specific proliferative and cytokine [gamma-interferon(IFN)-gamma] responses when challenged with autologous dendritic cells (DCs) loaded with HER2(776-788). By performing blocking studies with monoclonal antibodies (MAbs) and by using DCs from allogeneic donors sharing certain HLA-DR alleles, we found that HER2(776-788) is a promiscuous peptide presented, at least, by DRB5*0101, DRB1*0701 and DRB1*0405 alleles. One TCRV beta 6.7+ clone recognized the HLA-DRB5*0101+ FM3 melanoma cell line transfected with a full length HER-2/neu cDNA. Moreover, this clone recognized the HER-2/neu+ SKBR3 breast cancer cell line induced to express HLA-DR, thus demonstrating that HER2(776-788) represents a naturally processed and presented epitope. Our data demonstrate that helper peptide HER2(776-788) represents a promiscuous epitope binding to at least three HLA-DR alleles, thus offering a broad population coverage. The use of antigenic peptides presented by major histocompatibility complex (MHC) class II in addition to those presented by class I may improve the therapeutic efficacy of active immunization.
Journal Article
Research Support, Non-U.S. Gov't
Index Terms: Sciences bio-médicales et agricoles, Antigen Presentation, Antigens, Neoplasm -- immunology, Cell Line, Cells, Cultured, Clone Cells, Epitopes, T-Lymphocyte -- immunology, HLA-DR Antigens -- physiology, Humans, Interferon-gamma -- biosynthesis, Lymphocyte Activation, Neoplasms -- immunology, Peptide Fragments -- immunology, Peptides -- immunology, Receptor, erbB-2 -- immunology, T-Lymphocytes, Cytotoxic -- immunology, Tumor Cells, Cultured, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article
URL: https://dipot.ulb.ac.be/dspace/bitstream/2013/142120/4/doi_125579.pdf
Availability: Open access content. Open access content
1 full-text file(s): info:eu-repo/semantics/openAccess
Note: 1 full-text file(s): application/pdf
Other Numbers: EQY oai:dipot.ulb.ac.be:2013/142120
From OAIster®, provided by the OCLC Cooperative.
Accession Number: edsoai.on1363739905
Database: OAIster
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