Electronic Resource
Peptide HER2(776-788) represents a naturally processed broad MHC class II-restricted T cell epitope.
| Title: | Peptide HER2(776-788) represents a naturally processed broad MHC class II-restricted T cell epitope. |
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| Source: | British Journal of Cancer, 85 (10 |
| Publisher Information: | 2001-11 |
| Added Details: | Sotiriadou, R Perez, S A Gritzapis, A D Sotiropoulou, Panagiota Echner, H Heinzel, S Mamalaki, A Pawelec, Graham Voelter, W Baxevanis, C N Papamichail, Michael |
| Document Type: | Electronic Resource |
| Abstract: | HER2/neu-derived peptides inducing MHC class II-restricted CD4+ T helper lymphocyte (Th) responses, although critical for tumour rejection, are not thoroughly characterized. Here, we report the generation and characterization of CD4+ T cell clones specifically recognizing a HER-2/neu-derived peptide (776-788) [designated HER2(776-788)]. Such clones yielded specific proliferative and cytokine [gamma-interferon(IFN)-gamma] responses when challenged with autologous dendritic cells (DCs) loaded with HER2(776-788). By performing blocking studies with monoclonal antibodies (MAbs) and by using DCs from allogeneic donors sharing certain HLA-DR alleles, we found that HER2(776-788) is a promiscuous peptide presented, at least, by DRB5*0101, DRB1*0701 and DRB1*0405 alleles. One TCRV beta 6.7+ clone recognized the HLA-DRB5*0101+ FM3 melanoma cell line transfected with a full length HER-2/neu cDNA. Moreover, this clone recognized the HER-2/neu+ SKBR3 breast cancer cell line induced to express HLA-DR, thus demonstrating that HER2(776-788) represents a naturally processed and presented epitope. Our data demonstrate that helper peptide HER2(776-788) represents a promiscuous epitope binding to at least three HLA-DR alleles, thus offering a broad population coverage. The use of antigenic peptides presented by major histocompatibility complex (MHC) class II in addition to those presented by class I may improve the therapeutic efficacy of active immunization. Journal Article Research Support, Non-U.S. Gov't info:eu-repo/semantics/published |
| Index Terms: | Sciences bio-médicales et agricoles, Antigen Presentation, Antigens, Neoplasm -- immunology, Cell Line, Cells, Cultured, Clone Cells, Epitopes, T-Lymphocyte -- immunology, HLA-DR Antigens -- physiology, Humans, Interferon-gamma -- biosynthesis, Lymphocyte Activation, Neoplasms -- immunology, Peptide Fragments -- immunology, Peptides -- immunology, Receptor, erbB-2 -- immunology, T-Lymphocytes, Cytotoxic -- immunology, Tumor Cells, Cultured, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| Availability: | Open access content. Open access content 1 full-text file(s): info:eu-repo/semantics/openAccess |
| Note: | 1 full-text file(s): application/pdf English |
| Other Numbers: | EQY oai:dipot.ulb.ac.be:2013/142120 uri/info:doi/10.1054/bjoc.2001.2089 uri/info:pii/S0007092001920890 uri/info:pmid/11720440 uri/info:pmcid/PMC2363935 1363739905 |
| Contributing Source: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| Accession Number: | edsoai.on1363739905 |
| Database: | OAIster |
| Description not available. |