Academic Journal
A human vascularized microtumor model of patient-derived colorectal cancer recapitulates clinical disease.
| Title: | A human vascularized microtumor model of patient-derived colorectal cancer recapitulates clinical disease. |
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| Authors: | Hachey SJ; Irvine Department of Molecular Biology and Biochemistry, University of California, Irvine, California., Sobrino A; Irvine Department of Molecular Biology and Biochemistry, University of California, Irvine, California., Lee JG; Irvine School of Medicine, University of California, Irvine, California., Jafari MD; Department of Surgery, Weill Cornell Medicine, New York, New York., Klempner SJ; Massachusetts General Hospital Cancer Center, Boston, Massachusetts., Puttock EJ; Irvine Department of Mathematics, University of California, Irvine, California., Edwards RA; Irvine School of Medicine, University of California, Irvine, California., Lowengrub JS; Irvine Department of Mathematics, University of California, Irvine, California., Waterman ML; Irvine Department of Microbiology and Molecular Genetics, University of California, Irvine, California., Zell JA; Irvine School of Medicine, University of California, Irvine, California., Hughes CCW; Irvine Department of Molecular Biology and Biochemistry, University of California, Irvine, California; Irvine Department of Biomedical Engineering, University of California, Irvine, California. Electronic address: cchughes@uci.edu. |
| Source: | Translational research : the journal of laboratory and clinical medicine [Transl Res] 2022 Dec 05. Date of Electronic Publication: 2022 Dec 05. |
| Publication Model: | Ahead of Print |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Elsevier Country of Publication: United States NLM ID: 101280339 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1810 (Electronic) Linking ISSN: 18781810 NLM ISO Abbreviation: Transl Res Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: New York, N.Y. : Elsevier, [2006]- |
| Abstract: | Accurately modeling tumor biology and testing novel therapies on patient-derived cells is critically important to developing therapeutic regimens personalized to a patient's specific disease. The vascularized microtumor (VMT), or "tumor-on-a-chip," is a physiologic preclinical cancer model that incorporates key features of the native human tumor microenvironment within a transparent microfluidic platform, allowing rapid drug screening in vitro. Herein we optimize methods for generating patient-derived VMT (pVMT) using fresh colorectal cancer (CRC) biopsies and surgical resections to test drug sensitivities at the individual patient level. In response to standard chemotherapy and TGF-βR1 inhibition, we observe heterogeneous responses between pVMT derived from 6 patient biopsies, with the pVMT recapitulating tumor growth, histological features, metabolic heterogeneity, and drug responses of actual CRC tumors. Our results suggest that a translational infrastructure providing rapid information from patient-derived tumor cells in the pVMT, as established in this study, will support efforts to improve patient outcomes. (Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| Entry Date(s): | Date Created: 20221208 Latest Revision: 20221225 |
| Update Code: | 20221226 |
| DOI: | 10.1016/j.trsl.2022.11.011 |
| PMID: | 36481562 |
| Database: | MEDLINE |
| ISSN: | 1878-1810 |
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| DOI: | 10.1016/j.trsl.2022.11.011 |