Academic Journal

An in vitro vascularized micro-tumor model of human colorectal cancer recapitulates in vivo responses to standard-of-care therapy.

Bibliographic Details
Title: An in vitro vascularized micro-tumor model of human colorectal cancer recapitulates in vivo responses to standard-of-care therapy.
Authors: Hachey SJ; Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA 92697, USA., Movsesyan S, Nguyen QH, Burton-Sojo G, Tankazyan A, Wu J, Hoang T, Zhao D, Wang S, Hatch MM, Celaya E, Gomez S, Chen GT, Davis RT, Nee K, Pervolarakis N, Lawson DA, Kessenbrock K, Lee AP, Lowengrub J, Waterman ML, Hughes CCW
Source: Lab on a chip [Lab Chip] 2021 Apr 07; Vol. 21 (7), pp. 1333-1351. Date of Electronic Publication: 2021 Feb 19.
Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
Language: English
Journal Info: Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101128948 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1473-0189 (Electronic) Linking ISSN: 14730189 NLM ISO Abbreviation: Lab Chip Subsets: MEDLINE
Imprint Name(s): Original Publication: Cambridge, UK : Royal Society of Chemistry, c2001-
MeSH Terms: Antineoplastic Agents*/pharmacology , Antineoplastic Agents*/therapeutic use , Colorectal Neoplasms*/drug therapy, Drug Evaluation, Preclinical ; Humans ; Lab-On-A-Chip Devices ; Tumor Microenvironment
Abstract: Around 95% of anti-cancer drugs that show promise during preclinical study fail to gain FDA-approval for clinical use. This failure of the preclinical pipeline highlights the need for improved, physiologically-relevant in vitro models that can better serve as reliable drug-screening and disease modeling tools. The vascularized micro-tumor (VMT) is a novel three-dimensional model system (tumor-on-a-chip) that recapitulates the complex human tumor microenvironment, including perfused vasculature, within a transparent microfluidic device, allowing real-time study of drug responses and tumor-stromal interactions. Here we have validated this microphysiological system (MPS) platform for the study of colorectal cancer (CRC), the second leading cause of cancer-related deaths, by showing that gene expression, tumor heterogeneity, and treatment responses in the VMT more closely model CRC tumor clinicopathology than current standard drug screening modalities, including 2-dimensional monolayer culture and 3-dimensional spheroids.
References: Integr Biol (Camb). 2014 May;6(5):555-63. (PMID: 24676392)
Cell. 2010 Apr 2;141(1):52-67. (PMID: 20371345)
Nat Biotechnol. 2006 Dec;24(12):1569-71. (PMID: 17115054)
Nat Biotechnol. 2018 Jun;36(5):411-420. (PMID: 29608179)
Nat Rev Cancer. 2009 Sep;9(9):665-74. (PMID: 19693095)
Nat Biotechnol. 2014 Apr;32(4):381-386. (PMID: 24658644)
Cell. 2019 Jun 13;177(7):1888-1902.e21. (PMID: 31178118)
Drug Resist Updat. 2012 Feb-Apr;15(1-2):39-49. (PMID: 22335920)
Tissue Eng Part A. 2016 Aug;22(15-16):1016-25. (PMID: 27392582)
J Cell Biol. 2012 Feb 20;196(4):395-406. (PMID: 22351925)
Nature. 2019 May;569(7757):503-508. (PMID: 31068700)
Oncotarget. 2017 Mar 14;8(11):17819-17832. (PMID: 28147318)
Adv Drug Deliv Rev. 2014 Dec 15;79-80:222-37. (PMID: 25305336)
Cancer Cell. 2012 Apr 17;21(4):488-503. (PMID: 22516258)
Nat Rev Mol Cell Biol. 2006 Mar;7(3):211-24. (PMID: 16496023)
Tissue Eng Part C Methods. 2013 Sep;19(9):730-7. (PMID: 23320912)
SLAS Discov. 2017 Jun;22(5):456-472. (PMID: 28520521)
Blood. 2007 Jun 1;109(11):4761-8. (PMID: 17327403)
Lab Chip. 2013 Jan 7;13(1):81-9. (PMID: 23090158)
J Clin Oncol. 2008 Apr 20;26(12):2006-12. (PMID: 18421053)
Nucleic Acids Res. 2001 Jan 1;29(1):308-11. (PMID: 11125122)
Sci Rep. 2016 Aug 23;6:31589. (PMID: 27549930)
CA Cancer J Clin. 2019 Jan;69(1):7-34. (PMID: 30620402)
Am J Pathol. 2001 Apr;158(4):1325-34. (PMID: 11290550)
Nat Cell Biol. 2010 May;12(5):468-76. (PMID: 20418870)
Nature. 2011 May 19;473(7347):298-307. (PMID: 21593862)
EMBO J. 2014 Jul 1;33(13):1454-73. (PMID: 24825347)
Nat Rev Cancer. 2015 May;15(5):311-6. (PMID: 25907221)
Biomaterials. 2017 Feb;116:118-129. (PMID: 27914984)
Cell Stem Cell. 2017 Sep 7;21(3):297-300. (PMID: 28886364)
Mol Ther. 2008 Apr;16(4):698-706. (PMID: 18362927)
Nucleic Acids Res. 2019 Jan 8;47(D1):D721-D728. (PMID: 30289549)
Nature. 2015 Oct 1;526(7571):131-5. (PMID: 26416748)
Cell. 2008 Jul 25;134(2):215-30. (PMID: 18662538)
Cancer Cell. 2012 Nov 13;22(5):571-84. (PMID: 23153532)
Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9719-23. (PMID: 11481457)
Sci Rep. 2016 Jul 28;6:30599. (PMID: 27465284)
Lab Chip. 2017 Jan 31;17(3):511-520. (PMID: 28092382)
Nat Rev Clin Oncol. 2011 Mar 30;8(4):189-90. (PMID: 21448176)
Lab Chip. 2018 Sep 26;18(19):2893-2912. (PMID: 30156248)
Eur J Gastroenterol Hepatol. 2001 Apr;13(4):405-11. (PMID: 11338071)
Nature. 2005 Apr 14;434(7035):843-50. (PMID: 15829953)
Oncol Lett. 2017 Apr;13(4):2230-2236. (PMID: 28454385)
Nucleic Acids Res. 2019 Jun 20;47(11):e66. (PMID: 30923815)
Lab Chip. 2013 Aug 7;13(15):2990-8. (PMID: 23723013)
Nat Med. 2003 Jun;9(6):685-93. (PMID: 12778167)
EMBO Rep. 2014 Dec;15(12):1243-53. (PMID: 25381661)
Cell Death Dis. 2017 Jul 27;8(7):e2961. (PMID: 28749462)
Exp Biol Med (Maywood). 2014 Sep;239(9):1240-54. (PMID: 24740872)
Grant Information: R01 CA180122 United States CA NCI NIH HHS; U54 CA217378 United States CA NCI NIH HHS; UH2 TR000481 United States TR NCATS NIH HHS; UH3 TR000481 United States TR NCATS NIH HHS
Substance Nomenclature: 0 (Antineoplastic Agents)
Entry Date(s): Date Created: 20210219 Date Completed: 20210621 Latest Revision: 20211114
Update Code: 20221216
PubMed Central ID: PMC8525497
DOI: 10.1039/d0lc01216e
PMID: 33605955
Database: MEDLINE