Gnotobiotic system for selecting microorganisms with biocontrol potential against Fusarium oxysporum f. sp. physali
The cape gooseberry (Physalis peruviana) is a Solanaceae species with enormous economic importance in Colombia; it is the second most exported fruit, after bananas. Vascular wilt caused by Fusarium oxysporum f. sp. physali (Fox) is the most limiting factor of this crop, with losses of up to 80% of...
| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | Español |
| Publicado: |
Universidad Pedagógica y Tecnológica de Colombia - UPTC
2024
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| Acceso en línea: | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/11699 http://hdl.handle.net/20.500.12324/38973 |
| Sumario: | The cape gooseberry (Physalis peruviana) is a Solanaceae species with enormous economic importance in
Colombia; it is the second most exported fruit, after bananas. Vascular wilt caused by Fusarium oxysporum f.
sp. physali (Fox) is the most limiting factor of this crop, with losses of up to 80% of production. Biological
control is a promising alternative for controlling this pathogen. Bacteria and fungi, originally isolated from
potentially suppressive soils of cape gooseberry crops in Nariño, Colombia with different management (organic and conventional), were evaluated as biocontrol agents of Fox using a gnotobiotic model (seedlings
cultured under axenic conditions with defined microbial strains). Of the 64 isolated microorganisms, 37.5%
(15 bacteria and 9 fungi) were discarded because of toxicological risks and an unknow potential biological
control. The remaining 62.5% of the microorganisms, 14 bacteria and 26 fungi, were evaluated to assess their
potential as biological control agents against Fox. The gnotobiotic model system evaluated the protection and
plant growth promotion characteristics. Response variables were used to group the microorganism using a
principal component analysis (PCA), and five clusters were obtained. Cluster number four concentrated the
10 microorganisms (three bacteria and seven fungi) with the highest protection values against Fox, with a
positive effect on growth. The isolates were identified as two Bacillus subtilis strains, Rhodococcus sp., Podospora
setosa, Debaryomyces vindobonensis, Plectosphaerella plurivora, Acinetobacter rhizosphaerae, Umbelopsis sp. and two
strains of Trichoderma koningiopsis. The gnobiotic system offered clear advantages for evaluating and selecting
microorganisms with a biological control potential against Fusarium oxysporum f. sp. physalis. |
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